PubMed:16741917
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/16741917","sourcedb":"PubMed","sourceid":"16741917","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/16741917","text":"The DU145 human prostate carcinoma cell line harbors a temperature-sensitive allele of p53.\nBACKGROUND: Some nuances of mutant and wild-type p53 activity have been uncovered utilizing temperature sensitive (TS) alleles. However, few human tumor derived cell lines possess a TS p53 mutant.\nMETHODS: The cell lines DU145 (heterozygous p53, P223L, and V274F) and PC3 (p53-null, where exogenous P223L and V274F were introduced individually or in combination) were examined for TS p53 activity as revealed by reporter construct and target gene activation.\nRESULTS: TS p53 function was observed in DU145 and expression of the P223L allele in PC3 conferred a TS p53 profile. Activation of p21Waf1 demonstrated that P223L TS activity may have been influenced by cellular context.\nCONCLUSIONS: The DU145 cell line harbors a TS mutant of p53 and, in addition to being a widely used model of human prostate carcinoma, may also reveal new insights into p53 function due to the unique transcriptional properties of its TS 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