| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-46 |
Sentence |
denotes |
Characterization of gastric mucosal membranes. |
| T2 |
47-52 |
Sentence |
denotes |
VIII. |
| T3 |
53-116 |
Sentence |
denotes |
The localization of peptides by iodination and phosphorylation. |
| T4 |
117-288 |
Sentence |
denotes |
Two fractions of gastric mucosal membranes obtained by Ficoll-sucrose density gradient centrifugation were studied by a variety of techniques to localize the polypeptides. |
| T5 |
289-379 |
Sentence |
denotes |
Gel electrophoresis showed the presence of five major polypeptides and several minor ones. |
| T6 |
380-465 |
Sentence |
denotes |
Only one of these, 82,000 daltons, was available for iodination in the intact tissue. |
| T7 |
466-539 |
Sentence |
denotes |
The two membrane fractions differed in their accessibility to peroxidase. |
| T8 |
540-632 |
Sentence |
denotes |
The denser fraction showed two major defined iodination peaks at 82,000 and 102,000 daltons. |
| T9 |
633-821 |
Sentence |
denotes |
Freeze-thawing and iodinating with 131-I produced additional labeling of peaks as well as relabeling the 82,000-dalton component, showing it was accessible from both sides of the membrane. |
| T10 |
822-954 |
Sentence |
denotes |
The two major components were also sensitive to cross-linking, the 102,000 polypeptide being especially sensitive to --SH oxidation. |
| T11 |
955-1126 |
Sentence |
denotes |
Proteolysis with trypsin removed both components in the denser membrane fraction, in addition to inhibiting the K+-ATPase and K+-p-nitrophenylphosphatase of that fraction. |
| T12 |
1127-1278 |
Sentence |
denotes |
Phosphorylation with [gamma-32-P]ATP labeled the 102,000-dalton component and K+, HCO3- minus and p-nitrophenylphosphate reduced the level of labeling. |
| T13 |
1279-1445 |
Sentence |
denotes |
Hence the 102,000 region contains a subunit of the ATPase, is readily iodinated in inside-out vesicles, and is the most available for interpeptide S--S cross-linking. |
| T1 |
0-46 |
Sentence |
denotes |
Characterization of gastric mucosal membranes. |
| T2 |
47-52 |
Sentence |
denotes |
VIII. |
| T3 |
53-116 |
Sentence |
denotes |
The localization of peptides by iodination and phosphorylation. |
| T4 |
117-288 |
Sentence |
denotes |
Two fractions of gastric mucosal membranes obtained by Ficoll-sucrose density gradient centrifugation were studied by a variety of techniques to localize the polypeptides. |
| T5 |
289-379 |
Sentence |
denotes |
Gel electrophoresis showed the presence of five major polypeptides and several minor ones. |
| T6 |
380-465 |
Sentence |
denotes |
Only one of these, 82,000 daltons, was available for iodination in the intact tissue. |
| T7 |
466-539 |
Sentence |
denotes |
The two membrane fractions differed in their accessibility to peroxidase. |
| T8 |
540-632 |
Sentence |
denotes |
The denser fraction showed two major defined iodination peaks at 82,000 and 102,000 daltons. |
| T9 |
633-821 |
Sentence |
denotes |
Freeze-thawing and iodinating with 131-I produced additional labeling of peaks as well as relabeling the 82,000-dalton component, showing it was accessible from both sides of the membrane. |
| T10 |
822-954 |
Sentence |
denotes |
The two major components were also sensitive to cross-linking, the 102,000 polypeptide being especially sensitive to --SH oxidation. |
| T11 |
955-1126 |
Sentence |
denotes |
Proteolysis with trypsin removed both components in the denser membrane fraction, in addition to inhibiting the K+-ATPase and K+-p-nitrophenylphosphatase of that fraction. |
| T12 |
1127-1278 |
Sentence |
denotes |
Phosphorylation with [gamma-32-P]ATP labeled the 102,000-dalton component and K+, HCO3- minus and p-nitrophenylphosphate reduced the level of labeling. |
| T13 |
1279-1445 |
Sentence |
denotes |
Hence the 102,000 region contains a subunit of the ATPase, is readily iodinated in inside-out vesicles, and is the most available for interpeptide S--S cross-linking. |
