PubMed:1656972 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/1656972","sourcedb":"PubMed","sourceid":"1656972","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1656972","text":"Glycosylation of high-affinity thrombin receptors appears necessary for thrombin binding.\nMonosaccharide binding competition, lectin affinity chromatography, and glycosylation inhibitors have been used to determine if glycosylation plays a role in thrombin-receptor interactions. Mannose appeared to specifically inhibit thrombin binding to mouse embryo (ME) and hamster fibroblasts. Concanavalin A bound to antibody-purified receptor fractions, and was used as an affinity ligand to purify receptor fractions that retained thrombin binding activity. Cells treated with tunicamycin (6.25 ng/ml) for 24 h lost approximately 35% of their high-affinity thrombin binding sites, yet binding of receptor monoclonal antibody TR-9 was not affected, indicating that the receptor was present in the membrane, but unable to bind thrombin. Thus thrombin receptor glycosylation may be directly involved in thrombin binding.","tracks":[{"project":"bionlp-st-epi-2011-training","denotations":[{"id":"T1","span":{"begin":31,"end":39},"obj":"Protein"},{"id":"T2","span":{"begin":72,"end":80},"obj":"Protein"},{"id":"T3","span":{"begin":248,"end":256},"obj":"Protein"},{"id":"T4","span":{"begin":321,"end":329},"obj":"Protein"},{"id":"T5","span":{"begin":384,"end":398},"obj":"Protein"},{"id":"T6","span":{"begin":524,"end":532},"obj":"Protein"},{"id":"T7","span":{"begin":650,"end":658},"obj":"Protein"},{"id":"T8","span":{"begin":818,"end":826},"obj":"Protein"},{"id":"T9","span":{"begin":833,"end":841},"obj":"Protein"},{"id":"T10","span":{"begin":893,"end":901},"obj":"Protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T2","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T3","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T4","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T5","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T6","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T7","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T8","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T9","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T10","pred":"source","obj":"bionlp-st-epi-2011-training"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"bionlp-st-epi-2011-training","color":"#ecdf93","default":true}]}]}}