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PubMed:16455656 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-90 Sentence denotes CpSufE activates the cysteine desulfurase CpNifS for chloroplastic Fe-S cluster formation.
T2 91-310 Sentence denotes CpNifS, a cysteine desulfurase required to supply sulfur for ironsulfur cluster biogenesis in Arabidopsis thaliana chloroplasts, belongs to a class of NifS-like enzymes with low endogenous cysteine desulfurase activity.
T3 311-362 Sentence denotes Its bacterial homologue SufS is stimulated by SufE.
T4 363-527 Sentence denotes Here we characterize the Arabidopsis chloroplast protein CpSufE, which has an N-terminal SufE-like domain and a C-terminal BolA-like domain unique to higher plants.
T5 528-653 Sentence denotes CpSufE is targeted to the chloroplast stroma, indicated by green fluorescent protein localization and immunoblot experiments.
T6 654-747 Sentence denotes Like CpNifS, CpSufE is expressed in all major tissues, with higher expression in green parts.
T7 748-814 Sentence denotes Its expression is light-dependent and regulated at the mRNA level.
T8 815-997 Sentence denotes The addition of purified recombinant CpSufE increased the Vmax for the cysteine desulfurase activity of CpNifS over 40-fold and decreased the KM toward cysteine from 0.1 to 0.043 mm.
T9 998-1203 Sentence denotes In contrast, CpSufE addition decreased the affinity of CpNifS for selenocysteine, as indicated by an increase in the KM from 2.9 to 4.17 mm, and decreased the Vmax for selenocysteine lyase activity by 30%.
T10 1204-1330 Sentence denotes CpSufE forms dynamic complexes with CpNifS, indicated by gel filtration, native PAGE, and affinity chromatography experiments.
T11 1331-1478 Sentence denotes A mutant of CpSufE in which the single cysteine was changed to serine was not active in stimulating CpNifS, although it did compete with WT CpSufE.
T12 1479-1622 Sentence denotes The iron-sulfur cluster reconstitution activity of the CpNifS-CpSufE complex toward apoferredoxin was 20-fold higher than that of CpNifS alone.
T13 1623-1754 Sentence denotes We conclude that CpNifS and CpSufE together form a cysteine desulfurase required for iron-sulfur cluster formation in chloroplasts.

LocText

Id Subject Object Predicate Lexical cue
T1 0-6 uniprot:Q84W65 denotes CpSufE
T2 42-48 uniprot:O49543 denotes CpNifS
T3 53-66 go:GO:0009507 denotes chloroplastic
T4 91-97 uniprot:O49543 denotes CpNifS
T5 185-205 taxonomy:3702 denotes Arabidopsis thaliana
T6 206-218 go:GO:0009507 denotes chloroplasts
T7 335-339 uniprot:uniprot: denotes SufS
T8 357-361 uniprot:uniprot: denotes SufE
T9 388-399 taxonomy:3702 denotes Arabidopsis
T10 400-411 go:GO:0009507 denotes chloroplast
T11 420-426 uniprot:Q84W65 denotes CpSufE
T12 452-456 uniprot:uniprot: denotes SufE
T13 486-490 uniprot:uniprot: denotes BolA
T14 528-534 uniprot:Q84W65 denotes CpSufE
T15 554-572 go:GO:0009570 denotes chloroplast stroma
T16 587-612 uniprot:uniprot: denotes green fluorescent protein
T17 659-665 uniprot:O49543 denotes CpNifS
T18 667-673 uniprot:Q84W65 denotes CpSufE
T19 852-858 uniprot:Q84W65 denotes CpSufE
T20 919-925 uniprot:O49543 denotes CpNifS
T21 1011-1017 uniprot:Q84W65 denotes CpSufE
T22 1053-1059 uniprot:O49543 denotes CpNifS
T23 1204-1210 uniprot:Q84W65 denotes CpSufE
T24 1240-1246 uniprot:O49543 denotes CpNifS
T25 1343-1349 uniprot:Q84W65 denotes CpSufE
T26 1431-1437 uniprot:O49543 denotes CpNifS
T27 1471-1477 uniprot:Q84W65 denotes CpSufE
T28 1534-1540 uniprot:O49543 denotes CpNifS
T29 1541-1547 uniprot:Q84W65 denotes CpSufE
T30 1609-1615 uniprot:O49543 denotes CpNifS
T31 1640-1646 uniprot:O49543 denotes CpNifS
T32 1651-1657 uniprot:Q84W65 denotes CpSufE
T33 1741-1753 go:GO:0009507 denotes chloroplasts
R1 T2 T3 localizeTo CpNifS,chloroplastic
R2 T4 T6 localizeTo CpNifS,chloroplasts
R3 T11 T10 localizeTo CpSufE,chloroplast
R4 T14 T15 localizeTo CpSufE,chloroplast stroma
R5 T31 T33 localizeTo CpNifS,chloroplasts
R6 T32 T33 localizeTo CpSufE,chloroplasts

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 357-361 Disease denotes SufE http://purl.obolibrary.org/obo/MONDO_0018382
T2 452-456 Disease denotes SufE http://purl.obolibrary.org/obo/MONDO_0018382

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 185-205 OrganismTaxon denotes Arabidopsis thaliana 3702
T2 388-399 OrganismTaxon denotes Arabidopsis 3701
T3 513-526 OrganismTaxon denotes higher plants 3193

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 566-572 Body_part denotes stroma http://purl.obolibrary.org/obo/UBERON_0003891