PubMed:16440413 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/16440413","sourcedb":"PubMed","sourceid":"16440413","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/16440413","text":"Inhibitory effect of Polo-like kinase 1 depletion on mitosis and apoptosis of gastric cancer cells.\nAIM: Polo-like kinase 1 (PLK1) serine/threonine kinase plays a vital role in multiple phases of mitosis in gastric cancer cells. To investigate the effect of PLK1 depletion on mitosis and apoptosis of gastric cancer cells.\nMETHODS: PLK1 expression was blocked by small RNA interference(siRNA). The expression levels of PLK1, cdc2, cyclin B and caspase 3 were detected by Western blotting. Then, PLK1 depletion, cdc2 activity, cell proliferation, cell cycle phase distribution, mitotic spindle structure, and the rate of apoptosis of the PLK1 knockdown cells were observed.\nRESULTS: PLK1 gene knockdown was associated with increased cyclin B expression, increased cdc2 activity (but not with the expression levels), accumulation of gastric cancer cells at G2/M, improper mitotic spindle formation, delayed chromosome separation and delayed or arrested cytokinesis. Moreover, PLK1 depletion in gastric cancer cells was associated with decreased proliferation, attenuated pro-caspase 3 levels and increased apoptosis.\nCONCLUSION: Blockage of PLK1 expression may lead to decreased mitosis or even apoptosis in gastric cancer cells, indicating that PLK1 may be a valuable therapeutic target for gastric cancer.","tracks":[{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":21,"end":39},"obj":"gene:5347"},{"id":"T1","span":{"begin":78,"end":92},"obj":"disease:C0699791"},{"id":"T2","span":{"begin":21,"end":39},"obj":"gene:5347"},{"id":"T3","span":{"begin":78,"end":92},"obj":"disease:C0024623"},{"id":"T4","span":{"begin":258,"end":262},"obj":"gene:5347"},{"id":"T5","span":{"begin":301,"end":315},"obj":"disease:C0699791"},{"id":"T6","span":{"begin":258,"end":262},"obj":"gene:5347"},{"id":"T7","span":{"begin":301,"end":315},"obj":"disease:C0024623"},{"id":"T8","span":{"begin":332,"end":336},"obj":"gene:5347"},{"id":"T9","span":{"begin":831,"end":845},"obj":"disease:C0024623"},{"id":"T10","span":{"begin":763,"end":767},"obj":"gene:5424"},{"id":"T11","span":{"begin":831,"end":845},"obj":"disease:C0699791"},{"id":"T12","span":{"begin":763,"end":767},"obj":"gene:5424"},{"id":"T13","span":{"begin":831,"end":845},"obj":"disease:C0024623"},{"id":"T14","span":{"begin":763,"end":767},"obj":"gene:983"},{"id":"T15","span":{"begin":831,"end":845},"obj":"disease:C0024623"},{"id":"T16","span":{"begin":763,"end":767},"obj":"gene:983"},{"id":"T17","span":{"begin":831,"end":845},"obj":"disease:C0699791"},{"id":"T18","span":{"begin":332,"end":336},"obj":"gene:5347"},{"id":"T19","span":{"begin":831,"end":845},"obj":"disease:C0699791"},{"id":"T20","span":{"begin":974,"end":978},"obj":"gene:5347"},{"id":"T21","span":{"begin":992,"end":1006},"obj":"disease:C0699791"},{"id":"T22","span":{"begin":974,"end":978},"obj":"gene:5347"},{"id":"T23","span":{"begin":992,"end":1006},"obj":"disease:C0024623"},{"id":"T24","span":{"begin":1139,"end":1143},"obj":"gene:5347"},{"id":"T25","span":{"begin":1290,"end":1304},"obj":"disease:C0024623"},{"id":"T26","span":{"begin":1139,"end":1143},"obj":"gene:5347"},{"id":"T27","span":{"begin":1206,"end":1220},"obj":"disease:C0699791"},{"id":"T28","span":{"begin":1139,"end":1143},"obj":"gene:5347"},{"id":"T29","span":{"begin":1206,"end":1220},"obj":"disease:C0024623"},{"id":"T30","span":{"begin":1139,"end":1143},"obj":"gene:5347"},{"id":"T31","span":{"begin":1290,"end":1304},"obj":"disease:C0699791"},{"id":"T32","span":{"begin":1244,"end":1248},"obj":"gene:5347"},{"id":"T33","span":{"begin":1290,"end":1304},"obj":"disease:C0024623"},{"id":"T34","span":{"begin":1244,"end":1248},"obj":"gene:5347"},{"id":"T35","span":{"begin":1290,"end":1304},"obj":"disease:C0699791"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"},{"id":"R3","pred":"associated_with","subj":"T4","obj":"T5"},{"id":"R4","pred":"associated_with","subj":"T6","obj":"T7"},{"id":"R5","pred":"associated_with","subj":"T8","obj":"T9"},{"id":"R6","pred":"associated_with","subj":"T10","obj":"T11"},{"id":"R7","pred":"associated_with","subj":"T12","obj":"T13"},{"id":"R8","pred":"associated_with","subj":"T14","obj":"T15"},{"id":"R9","pred":"associated_with","subj":"T16","obj":"T17"},{"id":"R10","pred":"associated_with","subj":"T18","obj":"T19"},{"id":"R11","pred":"associated_with","subj":"T20","obj":"T21"},{"id":"R12","pred":"associated_with","subj":"T22","obj":"T23"},{"id":"R13","pred":"associated_with","subj":"T24","obj":"T25"},{"id":"R14","pred":"associated_with","subj":"T26","obj":"T27"},{"id":"R15","pred":"associated_with","subj":"T28","obj":"T29"},{"id":"R16","pred":"associated_with","subj":"T30","obj":"T31"},{"id":"R17","pred":"associated_with","subj":"T32","obj":"T33"},{"id":"R18","pred":"associated_with","subj":"T34","obj":"T35"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"attributes":[{"subj":"T0","pred":"source","obj":"DisGeNET"},{"subj":"T1","pred":"source","obj":"DisGeNET"},{"subj":"T2","pred":"source","obj":"DisGeNET"},{"subj":"T3","pred":"source","obj":"DisGeNET"},{"subj":"T4","pred":"source","obj":"DisGeNET"},{"subj":"T5","pred":"source","obj":"DisGeNET"},{"subj":"T6","pred":"source","obj":"DisGeNET"},{"subj":"T7","pred":"source","obj":"DisGeNET"},{"subj":"T8","pred":"source","obj":"DisGeNET"},{"subj":"T9","pred":"source","obj":"DisGeNET"},{"subj":"T10","pred":"source","obj":"DisGeNET"},{"subj":"T11","pred":"source","obj":"DisGeNET"},{"subj":"T12","pred":"source","obj":"DisGeNET"},{"subj":"T13","pred":"source","obj":"DisGeNET"},{"subj":"T14","pred":"source","obj":"DisGeNET"},{"subj":"T15","pred":"source","obj":"DisGeNET"},{"subj":"T16","pred":"source","obj":"DisGeNET"},{"subj":"T17","pred":"source","obj":"DisGeNET"},{"subj":"T18","pred":"source","obj":"DisGeNET"},{"subj":"T19","pred":"source","obj":"DisGeNET"},{"subj":"T20","pred":"source","obj":"DisGeNET"},{"subj":"T21","pred":"source","obj":"DisGeNET"},{"subj":"T22","pred":"source","obj":"DisGeNET"},{"subj":"T23","pred":"source","obj":"DisGeNET"},{"subj":"T24","pred":"source","obj":"DisGeNET"},{"subj":"T25","pred":"source","obj":"DisGeNET"},{"subj":"T26","pred":"source","obj":"DisGeNET"},{"subj":"T27","pred":"source","obj":"DisGeNET"},{"subj":"T28","pred":"source","obj":"DisGeNET"},{"subj":"T29","pred":"source","obj":"DisGeNET"},{"subj":"T30","pred":"source","obj":"DisGeNET"},{"subj":"T31","pred":"source","obj":"DisGeNET"},{"subj":"T32","pred":"source","obj":"DisGeNET"},{"subj":"T33","pred":"source","obj":"DisGeNET"},{"subj":"T34","pred":"source","obj":"DisGeNET"},{"subj":"T35","pred":"source","obj":"DisGeNET"}]},{"project":"DisGeNET5_gene_disease","denotations":[{"id":"16440413-0#21#39#gene5347","span":{"begin":21,"end":39},"obj":"gene5347"},{"id":"16440413-0#78#92#diseaseC0024623","span":{"begin":78,"end":92},"obj":"diseaseC0024623"},{"id":"16440413-0#78#92#diseaseC0699791","span":{"begin":78,"end":92},"obj":"diseaseC0699791"},{"id":"16440413-1#26#49#gene2011","span":{"begin":131,"end":154},"obj":"gene2011"},{"id":"16440413-1#102#116#diseaseC0024623","span":{"begin":207,"end":221},"obj":"diseaseC0024623"},{"id":"16440413-1#102#116#diseaseC0699791","span":{"begin":207,"end":221},"obj":"diseaseC0699791"},{"id":"16440413-6#50#56#gene5111","span":{"begin":732,"end":738},"obj":"gene5111"},{"id":"16440413-6#81#85#gene983","span":{"begin":763,"end":767},"obj":"gene983"},{"id":"16440413-6#81#85#gene5424","span":{"begin":763,"end":767},"obj":"gene5424"},{"id":"16440413-6#149#163#diseaseC0024623","span":{"begin":831,"end":845},"obj":"diseaseC0024623"},{"id":"16440413-6#149#163#diseaseC0699791","span":{"begin":831,"end":845},"obj":"diseaseC0699791"},{"id":"16440413-6#149#163#diseaseC0024623","span":{"begin":831,"end":845},"obj":"diseaseC0024623"},{"id":"16440413-6#149#163#diseaseC0699791","span":{"begin":831,"end":845},"obj":"diseaseC0699791"},{"id":"16440413-6#149#163#diseaseC0024623","span":{"begin":831,"end":845},"obj":"diseaseC0024623"},{"id":"16440413-6#149#163#diseaseC0699791","span":{"begin":831,"end":845},"obj":"diseaseC0699791"},{"id":"16440413-7#109#118#gene836","span":{"begin":1073,"end":1082},"obj":"gene836"},{"id":"16440413-7#28#42#diseaseC0024623","span":{"begin":992,"end":1006},"obj":"diseaseC0024623"},{"id":"16440413-7#28#42#diseaseC0699791","span":{"begin":992,"end":1006},"obj":"diseaseC0699791"}],"relations":[{"id":"21#39#gene534778#92#diseaseC0024623","pred":"associated_with","subj":"16440413-0#21#39#gene5347","obj":"16440413-0#78#92#diseaseC0024623"},{"id":"21#39#gene534778#92#diseaseC0699791","pred":"associated_with","subj":"16440413-0#21#39#gene5347","obj":"16440413-0#78#92#diseaseC0699791"},{"id":"26#49#gene2011102#116#diseaseC0024623","pred":"associated_with","subj":"16440413-1#26#49#gene2011","obj":"16440413-1#102#116#diseaseC0024623"},{"id":"26#49#gene2011102#116#diseaseC0699791","pred":"associated_with","subj":"16440413-1#26#49#gene2011","obj":"16440413-1#102#116#diseaseC0699791"},{"id":"50#56#gene5111149#163#diseaseC0024623","pred":"associated_with","subj":"16440413-6#50#56#gene5111","obj":"16440413-6#149#163#diseaseC0024623"},{"id":"50#56#gene5111149#163#diseaseC0699791","pred":"associated_with","subj":"16440413-6#50#56#gene5111","obj":"16440413-6#149#163#diseaseC0699791"},{"id":"50#56#gene5111149#163#diseaseC0024623","pred":"associated_with","subj":"16440413-6#50#56#gene5111","obj":"16440413-6#149#163#diseaseC0024623"},{"id":"50#56#gene5111149#163#diseaseC0699791","pred":"associated_with","subj":"16440413-6#50#56#gene5111","obj":"16440413-6#149#163#diseaseC0699791"},{"id":"50#56#gene5111149#163#diseaseC0024623","pred":"associated_with","subj":"16440413-6#50#56#gene5111","obj":"16440413-6#149#163#diseaseC0024623"},{"id":"50#56#gene5111149#163#diseaseC0699791","pred":"associated_with","subj":"16440413-6#50#56#gene5111","obj":"16440413-6#149#163#diseaseC0699791"},{"id":"81#85#gene983149#163#diseaseC0024623","pred":"associated_with","subj":"16440413-6#81#85#gene983","obj":"16440413-6#149#163#diseaseC0024623"},{"id":"81#85#gene983149#163#diseaseC0699791","pred":"associated_with","subj":"16440413-6#81#85#gene983","obj":"16440413-6#149#163#diseaseC0699791"},{"id":"81#85#gene983149#163#diseaseC0024623","pred":"associated_with","subj":"16440413-6#81#85#gene983","obj":"16440413-6#149#163#diseaseC0024623"},{"id":"81#85#gene983149#163#diseaseC0699791","pred":"associated_with","subj":"16440413-6#81#85#gene983","obj":"16440413-6#149#163#diseaseC0699791"},{"id":"81#85#gene983149#163#diseaseC0024623","pred":"associated_with","subj":"16440413-6#81#85#gene983","obj":"16440413-6#149#163#diseaseC0024623"},{"id":"81#85#gene983149#163#diseaseC0699791","pred":"associated_with","subj":"16440413-6#81#85#gene983","obj":"16440413-6#149#163#diseaseC0699791"},{"id":"81#85#gene5424149#163#diseaseC0024623","pred":"associated_with","subj":"16440413-6#81#85#gene5424","obj":"16440413-6#149#163#diseaseC0024623"},{"id":"81#85#gene5424149#163#diseaseC0699791","pred":"associated_with","subj":"16440413-6#81#85#gene5424","obj":"16440413-6#149#163#diseaseC0699791"},{"id":"81#85#gene5424149#163#diseaseC0024623","pred":"associated_with","subj":"16440413-6#81#85#gene5424","obj":"16440413-6#149#163#diseaseC0024623"},{"id":"81#85#gene5424149#163#diseaseC0699791","pred":"associated_with","subj":"16440413-6#81#85#gene5424","obj":"16440413-6#149#163#diseaseC0699791"},{"id":"81#85#gene5424149#163#diseaseC0024623","pred":"associated_with","subj":"16440413-6#81#85#gene5424","obj":"16440413-6#149#163#diseaseC0024623"},{"id":"81#85#gene5424149#163#diseaseC0699791","pred":"associated_with","subj":"16440413-6#81#85#gene5424","obj":"16440413-6#149#163#diseaseC0699791"},{"id":"109#118#gene83628#42#diseaseC0024623","pred":"associated_with","subj":"16440413-7#109#118#gene836","obj":"16440413-7#28#42#diseaseC0024623"},{"id":"109#118#gene83628#42#diseaseC0699791","pred":"associated_with","subj":"16440413-7#109#118#gene836","obj":"16440413-7#28#42#diseaseC0699791"}],"attributes":[{"subj":"16440413-0#21#39#gene5347","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-0#78#92#diseaseC0024623","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-0#78#92#diseaseC0699791","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-1#26#49#gene2011","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-1#102#116#diseaseC0024623","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-1#102#116#diseaseC0699791","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-6#50#56#gene5111","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-6#81#85#gene983","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-6#81#85#gene5424","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-6#149#163#diseaseC0024623","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-6#149#163#diseaseC0699791","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-6#149#163#diseaseC0024623","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-6#149#163#diseaseC0699791","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-6#149#163#diseaseC0024623","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-6#149#163#diseaseC0699791","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-7#109#118#gene836","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-7#28#42#diseaseC0024623","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"16440413-7#28#42#diseaseC0699791","pred":"source","obj":"DisGeNET5_gene_disease"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"DisGeNET","color":"#ecb693","default":true},{"id":"DisGeNET5_gene_disease","color":"#9c93ec"}]}]}}