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PubMed:16407284 JSONTXT

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PubMed_ArguminSci

Id Subject Object Predicate Lexical cue
T1 68-178 DRI_Approach denotes Activation of NFAT transcription factors requires their dephosphorylation by the phosphatase calcineurin (CN).
T2 179-261 DRI_Approach denotes NFATs contain two CN binding motifs: PxIxIT and CnBP-B/CNBR2 (which we call LxVP).
T3 262-399 DRI_Approach denotes Here we carry out a detailed comparative analysis of the CN binding activity displayed by the PxIxIT and LxVP sites from different NFATs.
T4 400-554 DRI_Outcome denotes Dose-response CN binding experiments with GST fusion proteins of NFATc1 and NFATc2 showed that NFATc1 binds CN in vitro more efficiently than does NFATc2.
T5 555-809 DRI_Challenge denotes This difference in binding appears to be caused by the different CN binding potencies of the corresponding LxVP sites; thus while the LxVPc2 peptide fused to GST did not bind CN, GST-LxVPc1 bound it more efficiently than did GST-PxIxITc1 or GST-PxIxITc2.
T6 810-951 DRI_Outcome denotes Furthermore, an NFATc2 chimera protein containing the LxVP motif from NFATc1 interacted with CN much more potently than did wild-type NFATc2.
T7 952-1098 DRI_Outcome denotes Free peptides spanning the LxVP motifs from NFATc1, c3 or c4 displaced CN from GST-NFATc1 and GST-NFATc2 more efficiently than any PxIxIT peptide.
T8 1099-1145 DRI_Background denotes PxIxITc2 and LxVPc1 peptides were each able to
T9 1174-1202 DRI_Background denotes and GST-PxIxITc2-CN binding.
T10 1203-1332 DRI_Outcome denotes In contrast with PxIxITc2, the LxVP peptide not only blocked CN-NFAT binding but also inhibited CN phosphatase activity in vitro.
T11 1333-1428 DRI_Background denotes Furthermore, exogenous LxVPc1 blocked NFATc2 phosphorylation and nuclear translocation in vivo.
T12 1429-1641 DRI_Background denotes These results suggest a model in which the different CN binding characteristics of the PxIxIT and LxVP sites enable different NFAT members to influence each others activities in cells where they are co-expressed.

sentences

Id Subject Object Predicate Lexical cue
T1 0-67 Sentence denotes Blockade of NFAT activation by the second calcineurin binding site.
T2 68-178 Sentence denotes Activation of NFAT transcription factors requires their dephosphorylation by the phosphatase calcineurin (CN).
T3 179-215 Sentence denotes NFATs contain two CN binding motifs:
T4 216-261 Sentence denotes PxIxIT and CnBP-B/CNBR2 (which we call LxVP).
T5 262-399 Sentence denotes Here we carry out a detailed comparative analysis of the CN binding activity displayed by the PxIxIT and LxVP sites from different NFATs.
T6 400-554 Sentence denotes Dose-response CN binding experiments with GST fusion proteins of NFATc1 and NFATc2 showed that NFATc1 binds CN in vitro more efficiently than does NFATc2.
T7 555-809 Sentence denotes This difference in binding appears to be caused by the different CN binding potencies of the corresponding LxVP sites; thus while the LxVPc2 peptide fused to GST did not bind CN, GST-LxVPc1 bound it more efficiently than did GST-PxIxITc1 or GST-PxIxITc2.
T8 810-951 Sentence denotes Furthermore, an NFATc2 chimera protein containing the LxVP motif from NFATc1 interacted with CN much more potently than did wild-type NFATc2.
T9 952-1098 Sentence denotes Free peptides spanning the LxVP motifs from NFATc1, c3 or c4 displaced CN from GST-NFATc1 and GST-NFATc2 more efficiently than any PxIxIT peptide.
T10 1099-1202 Sentence denotes PxIxITc2 and LxVPc1 peptides were each able to cross-compete GST-LxVPc1-CN and GST-PxIxITc2-CN binding.
T11 1203-1332 Sentence denotes In contrast with PxIxITc2, the LxVP peptide not only blocked CN-NFAT binding but also inhibited CN phosphatase activity in vitro.
T12 1333-1428 Sentence denotes Furthermore, exogenous LxVPc1 blocked NFATc2 phosphorylation and nuclear translocation in vivo.
T13 1429-1641 Sentence denotes These results suggest a model in which the different CN binding characteristics of the PxIxIT and LxVP sites enable different NFAT members to influence each others activities in cells where they are co-expressed.

bionlp-st-pc-2013-training

Id Subject Object Predicate Lexical cue
T1 12-16 Gene_or_gene_product denotes NFAT
T2 42-53 Complex denotes calcineurin
T3 82-86 Gene_or_gene_product denotes NFAT
T4 161-172 Complex denotes calcineurin
T5 174-176 Complex denotes CN
T6 179-184 Gene_or_gene_product denotes NFATs
T7 197-199 Complex denotes CN
T8 319-321 Complex denotes CN
T9 393-398 Gene_or_gene_product denotes NFATs
T10 414-416 Complex denotes CN
T11 442-445 Gene_or_gene_product denotes GST
T12 465-471 Gene_or_gene_product denotes NFATc1
T13 476-482 Gene_or_gene_product denotes NFATc2
T14 495-501 Gene_or_gene_product denotes NFATc1
T15 508-510 Complex denotes CN
T16 547-553 Gene_or_gene_product denotes NFATc2
T17 620-622 Complex denotes CN
T18 689-695 Gene_or_gene_product denotes LxVPc2
T19 713-716 Gene_or_gene_product denotes GST
T20 730-732 Complex denotes CN
T21 734-744 Gene_or_gene_product denotes GST-LxVPc1
T22 780-792 Gene_or_gene_product denotes GST-PxIxITc1
T23 796-808 Gene_or_gene_product denotes GST-PxIxITc2
T24 826-832 Gene_or_gene_product denotes NFATc2
T25 880-886 Gene_or_gene_product denotes NFATc1
T26 903-905 Complex denotes CN
T27 944-950 Gene_or_gene_product denotes NFATc2
T28 996-1002 Gene_or_gene_product denotes NFATc1
T29 1004-1006 Gene_or_gene_product denotes c3
T30 1010-1012 Gene_or_gene_product denotes c4
T31 1023-1025 Complex denotes CN
T32 1031-1041 Complex denotes GST-NFATc1
T33 1046-1056 Complex denotes GST-NFATc2
T34 1083-1089 Gene_or_gene_product denotes PxIxIT
T35 1099-1107 Gene_or_gene_product denotes PxIxITc2
T36 1112-1118 Gene_or_gene_product denotes LxVPc1
T37 1160-1173 Complex denotes GST-LxVPc1-CN
T38 1178-1193 Complex denotes GST-PxIxITc2-CN
T39 1220-1228 Gene_or_gene_product denotes PxIxITc2
T40 1234-1238 Gene_or_gene_product denotes LxVP
T41 1264-1266 Complex denotes CN
T42 1267-1271 Gene_or_gene_product denotes NFAT
T43 1299-1301 Complex denotes CN
T44 1356-1362 Gene_or_gene_product denotes LxVPc1
T45 1371-1377 Gene_or_gene_product denotes NFATc2
T46 1398-1405 Cellular_component denotes nuclear
T47 1482-1484 Complex denotes CN
T48 1555-1559 Gene_or_gene_product denotes NFAT