PubMed:16306051
Annnotations
Glycan-Motif
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 157-168 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
| T2 | 172-188 | https://glytoucan.org/Structures/Glycans/G20420WT | denotes | lactosylceramide |
| T3 | 172-188 | https://glytoucan.org/Structures/Glycans/G74621DY | denotes | lactosylceramide |
| T4 | 172-188 | https://glytoucan.org/Structures/Glycans/G84224TW | denotes | lactosylceramide |
GlyCosmos6-Glycan-Motif-Image
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 157-168 | Glycan_Motif | denotes | sialic acid | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY |
| T2 | 172-188 | Glycan_Motif | denotes | lactosylceramide | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G84224TW|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G74621DY|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G20420WT |
GlyCosmos6-Glycan-Motif-Structure
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 157-168 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
| T2 | 172-188 | https://glytoucan.org/Structures/Glycans/G20420WT | denotes | lactosylceramide |
| T3 | 172-188 | https://glytoucan.org/Structures/Glycans/G74621DY | denotes | lactosylceramide |
| T4 | 172-188 | https://glytoucan.org/Structures/Glycans/G84224TW | denotes | lactosylceramide |
Glycosmos6-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 172-188 | http://www.glycoepitope.jp/epitopes/EP0078 | denotes | lactosylceramide |
GlycoBiology-PACDB
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| _T1 | 1764-1780 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC002,LEC056,LEC062,LEC069,LEC081,LEC111,LEC133,LEC171,LEC177,LEC187,LEC211,LEC242,LEC252,LEC258,LEC259,LEC260,LEC262,LEC369,LEC377,LEC422,LEC442,LEC448,LEC450,LEC451,LEC454,LEC472,LEC492,LEC620 | denotes | Escherichia coli |
| _T2 | 1764-1780 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC157,LEC407 | denotes | Escherichia coli |
| _T3 | 1764-1780 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC754 | denotes | Escherichia coli |
| _T4 | 1764-1780 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC243,LEC640 | denotes | Escherichia coli |
| _T5 | 1764-1780 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC295,LEC417 | denotes | Escherichia coli |
| _T6 | 1764-1780 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC487 | denotes | Escherichia coli |
| _T7 | 1764-1780 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC244,LEC256,LEC354 | denotes | Escherichia coli |
| _T8 | 1764-1780 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC054,LEC058,LEC073,LEC082,LEC091,LEC103,LEC109,LEC110,LEC123,LEC158,LEC179,LEC198,LEC205,LEC222,LEC223,LEC224,LEC225,LEC232,LEC298,LEC357,LEC378,LEC383,LEC388,LEC389,LEC397,LEC401,LEC410,LEC452 | denotes | Escherichia coli |
| _T9 | 1764-1780 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC636 | denotes | Escherichia coli |
uniprot-human
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 87-95 | http://www.uniprot.org/uniprot/Q9UNP4 | denotes | ST3Gal-V |
| T2 | 136-144 | http://www.uniprot.org/uniprot/Q9UNP4 | denotes | ST3Gal-V |
| T3 | 256-264 | http://www.uniprot.org/uniprot/Q9UNP4 | denotes | ST3Gal-V |
| T4 | 373-381 | http://www.uniprot.org/uniprot/Q9UNP4 | denotes | ST3Gal-V |
| T5 | 675-683 | http://www.uniprot.org/uniprot/Q9UNP4 | denotes | ST3Gal-V |
| T6 | 901-909 | http://www.uniprot.org/uniprot/Q9UNP4 | denotes | ST3Gal-V |
| T7 | 1249-1257 | http://www.uniprot.org/uniprot/Q9UNP4 | denotes | ST3Gal-V |
| T8 | 1671-1679 | http://www.uniprot.org/uniprot/Q9UNP4 | denotes | ST3Gal-V |
| T9 | 963-971 | http://www.uniprot.org/uniprot/Q11201 | denotes | ST3Gal-I |
uniprot-mouse
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 87-95 | http://www.uniprot.org/uniprot/Q9QWF9 | denotes | ST3Gal-V |
| T2 | 136-144 | http://www.uniprot.org/uniprot/Q9QWF9 | denotes | ST3Gal-V |
| T3 | 256-264 | http://www.uniprot.org/uniprot/Q9QWF9 | denotes | ST3Gal-V |
| T4 | 373-381 | http://www.uniprot.org/uniprot/Q9QWF9 | denotes | ST3Gal-V |
| T5 | 675-683 | http://www.uniprot.org/uniprot/Q9QWF9 | denotes | ST3Gal-V |
| T6 | 901-909 | http://www.uniprot.org/uniprot/Q9QWF9 | denotes | ST3Gal-V |
| T7 | 1249-1257 | http://www.uniprot.org/uniprot/Q9QWF9 | denotes | ST3Gal-V |
| T8 | 1671-1679 | http://www.uniprot.org/uniprot/Q9QWF9 | denotes | ST3Gal-V |
| T9 | 963-971 | http://www.uniprot.org/uniprot/P54751 | denotes | ST3Gal-I |
GlycoBiology-NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 350-355 | http://purl.bioontology.org/ontology/STY/T025 | denotes | cells |
| T2 | 935-942 | http://purl.bioontology.org/ontology/NCBITAXON/353209 | denotes | related |
| T3 | 1160-1169 | http://purl.bioontology.org/ontology/NCBITAXON/67440 | denotes | tetraodon |
| T4 | 1160-1169 | http://purl.bioontology.org/ontology/NCBITAXON/47144 | denotes | tetraodon |
| T5 | 1764-1775 | http://purl.bioontology.org/ontology/NCBITAXON/561 | denotes | Escherichia |
GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 303-318 | http://purl.obolibrary.org/obo/GO_0003824 | denotes | enzyme activity |
| T2 | 607-622 | http://purl.obolibrary.org/obo/GO_0003824 | denotes | enzyme activity |
| T3 | 345-348 | http://purl.obolibrary.org/obo/GO_0043848 | denotes | CHO |
| T4 | 555-567 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | glycosylated |
| T5 | 652-665 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | glycosylation |
| T6 | 1016-1029 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | glycosylation |
| T7 | 1503-1516 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | glycosylation |
GO-CC
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 350-355 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| TextSentencer_T1 | 0-114 | Sentence | denotes | Substitution of the N-glycan function in glycosyltransferases by specific amino acids: ST3Gal-V as a model enzyme. |
| TextSentencer_T2 | 115-189 | Sentence | denotes | The sialyltranferase ST3Gal-V transfers a sialic acid to lactosylceramide. |
| TextSentencer_T3 | 190-402 | Sentence | denotes | We investigated the role of each of the N-glycans modifying mouse ST3Gal-V (mST3Gal-V) by measuring the in vitro enzyme activity of Chinese hamster ovary (CHO) cells transfected with ST3Gal-V cDNA or its mutants. |
| TextSentencer_T4 | 403-623 | Sentence | denotes | By examining mutants of mST3Gal-V, in which each asparagine was replaced with glutamine (N180Q, N224Q, N334Q), we determined that all three sites are N-glycosylated and that each N-glycan is required for enzyme activity. |
| TextSentencer_T5 | 624-716 | Sentence | denotes | Despite their importance, N-glycosylation sites in ST3Gal-V are not conserved among species. |
| TextSentencer_T6 | 717-1036 | Sentence | denotes | Therefore, we considered whether the function in the activity that is performed in mST3Gal-V by the N-glycan could be substituted for by specific amino acid residues selected from the ST3Gal-V of other species or from related sialyltransferases (ST3Gal-I, -II, -III, and -IV), placed at or near the glycosylation sites. |
| TextSentencer_T7 | 1037-1244 | Sentence | denotes | To this end, we constructed a series of interspecies mutants for mST3Gal-V, specifically, mST3Gal-V-H177D-N180S (medaka or tetraodon type), mST3Gal-V-N224K (human type), and mST3Gal-V-T336Q (zebrafish type). |
| TextSentencer_T8 | 1245-1334 | Sentence | denotes | The ST3Gal-V activity of these mutants was quite similar to that of the wild-type enzyme. |
| TextSentencer_T9 | 1335-1523 | Sentence | denotes | Thus, we have demonstrated here that the N-glycans on mST3Gal-V are required for activity but can be substituted for specific amino acid residues placed at or near the glycosylation sites. |
| TextSentencer_T10 | 1524-1636 | Sentence | denotes | We named this method SUNGA (substitution of N-glycan functions in glycosyltransferases by specific amino acids). |
| TextSentencer_T11 | 1637-1884 | Sentence | denotes | Furthermore, we verified that the ST3Gal-V mutant created using the SUNGA method maintains its high activity when expressed in Escherichia coli thereby establishing the usefulness of the SUNGA method in exploring the function of N-glycans in vivo. |
| T1 | 0-114 | Sentence | denotes | Substitution of the N-glycan function in glycosyltransferases by specific amino acids: ST3Gal-V as a model enzyme. |
| T2 | 115-189 | Sentence | denotes | The sialyltranferase ST3Gal-V transfers a sialic acid to lactosylceramide. |
| T3 | 190-402 | Sentence | denotes | We investigated the role of each of the N-glycans modifying mouse ST3Gal-V (mST3Gal-V) by measuring the in vitro enzyme activity of Chinese hamster ovary (CHO) cells transfected with ST3Gal-V cDNA or its mutants. |
| T4 | 403-623 | Sentence | denotes | By examining mutants of mST3Gal-V, in which each asparagine was replaced with glutamine (N180Q, N224Q, N334Q), we determined that all three sites are N-glycosylated and that each N-glycan is required for enzyme activity. |
| T5 | 624-716 | Sentence | denotes | Despite their importance, N-glycosylation sites in ST3Gal-V are not conserved among species. |
| T6 | 717-1036 | Sentence | denotes | Therefore, we considered whether the function in the activity that is performed in mST3Gal-V by the N-glycan could be substituted for by specific amino acid residues selected from the ST3Gal-V of other species or from related sialyltransferases (ST3Gal-I, -II, -III, and -IV), placed at or near the glycosylation sites. |
| T7 | 1037-1244 | Sentence | denotes | To this end, we constructed a series of interspecies mutants for mST3Gal-V, specifically, mST3Gal-V-H177D-N180S (medaka or tetraodon type), mST3Gal-V-N224K (human type), and mST3Gal-V-T336Q (zebrafish type). |
| T8 | 1245-1334 | Sentence | denotes | The ST3Gal-V activity of these mutants was quite similar to that of the wild-type enzyme. |
| T9 | 1335-1523 | Sentence | denotes | Thus, we have demonstrated here that the N-glycans on mST3Gal-V are required for activity but can be substituted for specific amino acid residues placed at or near the glycosylation sites. |
| T10 | 1524-1636 | Sentence | denotes | We named this method SUNGA (substitution of N-glycan functions in glycosyltransferases by specific amino acids). |
| T11 | 1637-1884 | Sentence | denotes | Furthermore, we verified that the ST3Gal-V mutant created using the SUNGA method maintains its high activity when expressed in Escherichia coli thereby establishing the usefulness of the SUNGA method in exploring the function of N-glycans in vivo. |
| T1 | 0-114 | Sentence | denotes | Substitution of the N-glycan function in glycosyltransferases by specific amino acids: ST3Gal-V as a model enzyme. |
| T2 | 115-189 | Sentence | denotes | The sialyltranferase ST3Gal-V transfers a sialic acid to lactosylceramide. |
| T3 | 190-402 | Sentence | denotes | We investigated the role of each of the N-glycans modifying mouse ST3Gal-V (mST3Gal-V) by measuring the in vitro enzyme activity of Chinese hamster ovary (CHO) cells transfected with ST3Gal-V cDNA or its mutants. |
| T4 | 403-623 | Sentence | denotes | By examining mutants of mST3Gal-V, in which each asparagine was replaced with glutamine (N180Q, N224Q, N334Q), we determined that all three sites are N-glycosylated and that each N-glycan is required for enzyme activity. |
| T5 | 624-716 | Sentence | denotes | Despite their importance, N-glycosylation sites in ST3Gal-V are not conserved among species. |
| T6 | 717-1036 | Sentence | denotes | Therefore, we considered whether the function in the activity that is performed in mST3Gal-V by the N-glycan could be substituted for by specific amino acid residues selected from the ST3Gal-V of other species or from related sialyltransferases (ST3Gal-I, -II, -III, and -IV), placed at or near the glycosylation sites. |
| T7 | 1037-1244 | Sentence | denotes | To this end, we constructed a series of interspecies mutants for mST3Gal-V, specifically, mST3Gal-V-H177D-N180S (medaka or tetraodon type), mST3Gal-V-N224K (human type), and mST3Gal-V-T336Q (zebrafish type). |
| T8 | 1245-1334 | Sentence | denotes | The ST3Gal-V activity of these mutants was quite similar to that of the wild-type enzyme. |
| T9 | 1335-1523 | Sentence | denotes | Thus, we have demonstrated here that the N-glycans on mST3Gal-V are required for activity but can be substituted for specific amino acid residues placed at or near the glycosylation sites. |
| T10 | 1524-1636 | Sentence | denotes | We named this method SUNGA (substitution of N-glycan functions in glycosyltransferases by specific amino acids). |
| T11 | 1637-1884 | Sentence | denotes | Furthermore, we verified that the ST3Gal-V mutant created using the SUNGA method maintains its high activity when expressed in Escherichia coli thereby establishing the usefulness of the SUNGA method in exploring the function of N-glycans in vivo. |
GlycoBiology-Motifs
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 20-28 | http://rdf.glycoinfo.org/glycan/G00027MO | denotes | N-glycan |
| T2 | 582-590 | http://rdf.glycoinfo.org/glycan/G00027MO | denotes | N-glycan |
| T3 | 817-825 | http://rdf.glycoinfo.org/glycan/G00027MO | denotes | N-glycan |
| T4 | 1568-1576 | http://rdf.glycoinfo.org/glycan/G00027MO | denotes | N-glycan |
| T5 | 1866-1875 | http://rdf.glycoinfo.org/glycan/G00027MO | denotes | N-glycans |
GlycoBiology-Epitope
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| PD-GlycoEpitope-B_T1 | 172-188 | http://www.glycoepitope.jp/epitopes/EP0078 | denotes | lactosylceramide |
GlyTouCan-IUPAC
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| GlycanIUPAC_T1 | 533-536 | "http://rdf.glycoinfo.org/glycan/G41652MJ" | denotes | all |
| GlycanIUPAC_T2 | 533-536 | "http://rdf.glycoinfo.org/glycan/G20761YC" | denotes | all |
| GlycanIUPAC_T3 | 533-536 | "http://rdf.glycoinfo.org/glycan/G19807HM" | denotes | all |
| GlycanIUPAC_T4 | 533-536 | "http://rdf.glycoinfo.org/glycan/G20351TE" | denotes | all |
| GlycanIUPAC_T5 | 533-536 | "http://rdf.glycoinfo.org/glycan/G71957MR" | denotes | all |
| GlycanIUPAC_T6 | 533-536 | "http://rdf.glycoinfo.org/glycan/G59040AE" | denotes | all |
| GlycanIUPAC_T7 | 533-536 | "http://rdf.glycoinfo.org/glycan/G14987PW" | denotes | all |
| GlycanIUPAC_T8 | 533-536 | "http://rdf.glycoinfo.org/glycan/G95064PC" | denotes | all |
| GlycanIUPAC_T9 | 533-536 | "http://rdf.glycoinfo.org/glycan/G39143AQ" | denotes | all |
| GlycanIUPAC_T10 | 533-536 | "http://rdf.glycoinfo.org/glycan/G65149OO" | denotes | all |
| GlycanIUPAC_T11 | 533-536 | "http://rdf.glycoinfo.org/glycan/G02766SY" | denotes | all |
| GlycanIUPAC_T12 | 533-536 | "http://rdf.glycoinfo.org/glycan/G26019KJ" | denotes | all |
| GlycanIUPAC_T13 | 533-536 | "http://rdf.glycoinfo.org/glycan/G36429CZ" | denotes | all |
| GlycanIUPAC_T14 | 533-536 | "http://rdf.glycoinfo.org/glycan/G89633TP" | denotes | all |
| GlycanIUPAC_T15 | 533-536 | "http://rdf.glycoinfo.org/glycan/G28494FO" | denotes | all |
| GlycanIUPAC_T16 | 533-536 | "http://rdf.glycoinfo.org/glycan/G06219CP" | denotes | all |
| GlycanIUPAC_T17 | 533-536 | "http://rdf.glycoinfo.org/glycan/G44237SM" | denotes | all |
| GlycanIUPAC_T18 | 533-536 | "http://rdf.glycoinfo.org/glycan/G57948RL" | denotes | all |
| GlycanIUPAC_T19 | 533-536 | "http://rdf.glycoinfo.org/glycan/G64016DN" | denotes | all |
| GlycanIUPAC_T20 | 533-536 | "http://rdf.glycoinfo.org/glycan/G14536PC" | denotes | all |
| GlycanIUPAC_T21 | 533-536 | "http://rdf.glycoinfo.org/glycan/G14356FW" | denotes | all |
| GlycanIUPAC_T22 | 533-536 | "http://rdf.glycoinfo.org/glycan/G34565UO" | denotes | all |
| GlycanIUPAC_T23 | 533-536 | "http://rdf.glycoinfo.org/glycan/G67124MW" | denotes | all |
| GlycanIUPAC_T24 | 533-536 | "http://rdf.glycoinfo.org/glycan/G71457ZU" | denotes | all |
| GlycanIUPAC_T25 | 533-536 | "http://rdf.glycoinfo.org/glycan/G55228VZ" | denotes | all |
| GlycanIUPAC_T26 | 533-536 | "http://rdf.glycoinfo.org/glycan/G31034MJ" | denotes | all |
| GlycanIUPAC_T27 | 533-536 | "http://rdf.glycoinfo.org/glycan/G25776IP" | denotes | all |
| GlycanIUPAC_T28 | 533-536 | "http://rdf.glycoinfo.org/glycan/G64442BV" | denotes | all |
| GlycanIUPAC_T29 | 533-536 | "http://rdf.glycoinfo.org/glycan/G57018LE" | denotes | all |
| GlycanIUPAC_T30 | 533-536 | "http://rdf.glycoinfo.org/glycan/G61761GX" | denotes | all |
| GlycanIUPAC_T31 | 533-536 | "http://rdf.glycoinfo.org/glycan/G76318UX" | denotes | all |
| GlycanIUPAC_T32 | 533-536 | "http://rdf.glycoinfo.org/glycan/G61906ER" | denotes | all |
| GlycanIUPAC_T33 | 533-536 | "http://rdf.glycoinfo.org/glycan/G68723GR" | denotes | all |
| GlycanIUPAC_T34 | 533-536 | "http://rdf.glycoinfo.org/glycan/G19540LE" | denotes | all |
| GlycanIUPAC_T35 | 533-536 | "http://rdf.glycoinfo.org/glycan/G74944PO" | denotes | all |
| GlycanIUPAC_T36 | 533-536 | "http://rdf.glycoinfo.org/glycan/G89489ZJ" | denotes | all |
| GlycanIUPAC_T37 | 533-536 | "http://rdf.glycoinfo.org/glycan/G04434YU" | denotes | all |
| GlycanIUPAC_T38 | 533-536 | "http://rdf.glycoinfo.org/glycan/G21450PB" | denotes | all |
| GlycanIUPAC_T39 | 533-536 | "http://rdf.glycoinfo.org/glycan/G93629QY" | denotes | all |
| GlycanIUPAC_T40 | 533-536 | "http://rdf.glycoinfo.org/glycan/G02603TR" | denotes | all |
| GlycanIUPAC_T41 | 533-536 | "http://rdf.glycoinfo.org/glycan/G40280JP" | denotes | all |
| GlycanIUPAC_T42 | 533-536 | "http://rdf.glycoinfo.org/glycan/G95259IC" | denotes | all |
| GlycanIUPAC_T43 | 533-536 | "http://rdf.glycoinfo.org/glycan/G26900FE" | denotes | all |
| GlycanIUPAC_T44 | 533-536 | "http://rdf.glycoinfo.org/glycan/G21346KK" | denotes | all |
| GlycanIUPAC_T45 | 533-536 | "http://rdf.glycoinfo.org/glycan/G62509FF" | denotes | all |
| GlycanIUPAC_T46 | 533-536 | "http://rdf.glycoinfo.org/glycan/G83932AK" | denotes | all |
| GlycanIUPAC_T47 | 533-536 | "http://rdf.glycoinfo.org/glycan/G96978IB" | denotes | all |
| GlycanIUPAC_T48 | 533-536 | "http://rdf.glycoinfo.org/glycan/G34275DN" | denotes | all |
| GlycanIUPAC_T49 | 533-536 | "http://rdf.glycoinfo.org/glycan/G07071JF" | denotes | all |
| GlycanIUPAC_T50 | 533-536 | "http://rdf.glycoinfo.org/glycan/G80639QD" | denotes | all |
| GlycanIUPAC_T51 | 533-536 | "http://rdf.glycoinfo.org/glycan/G99460PJ" | denotes | all |
| GlycanIUPAC_T52 | 533-536 | "http://rdf.glycoinfo.org/glycan/G22024BZ" | denotes | all |
| GlycanIUPAC_T53 | 533-536 | "http://rdf.glycoinfo.org/glycan/G74097ZY" | denotes | all |
| GlycanIUPAC_T54 | 533-536 | "http://rdf.glycoinfo.org/glycan/G84439YP" | denotes | all |
| GlycanIUPAC_T55 | 533-536 | "http://rdf.glycoinfo.org/glycan/G52207WQ" | denotes | all |
| GlycanIUPAC_T56 | 533-536 | "http://rdf.glycoinfo.org/glycan/G90695MS" | denotes | all |
| GlycanIUPAC_T57 | 533-536 | "http://rdf.glycoinfo.org/glycan/G50398QX" | denotes | all |
| GlycanIUPAC_T58 | 533-536 | "http://rdf.glycoinfo.org/glycan/G12166ZT" | denotes | all |
| GlycanIUPAC_T59 | 533-536 | "http://rdf.glycoinfo.org/glycan/G48368BR" | denotes | all |
| GlycanIUPAC_T60 | 533-536 | "http://rdf.glycoinfo.org/glycan/G57407RW" | denotes | all |
| GlycanIUPAC_T61 | 533-536 | "http://rdf.glycoinfo.org/glycan/G00386TY" | denotes | all |
| GlycanIUPAC_T62 | 533-536 | "http://rdf.glycoinfo.org/glycan/G18723JK" | denotes | all |
| GlycanIUPAC_T63 | 533-536 | "http://rdf.glycoinfo.org/glycan/G93757OR" | denotes | all |
| GlycanIUPAC_T64 | 533-536 | "http://rdf.glycoinfo.org/glycan/G29006SI" | denotes | all |
| GlycanIUPAC_T65 | 533-536 | "http://rdf.glycoinfo.org/glycan/G03099OQ" | denotes | all |
| GlycanIUPAC_T66 | 533-536 | "http://rdf.glycoinfo.org/glycan/G53739OW" | denotes | all |
| GlycanIUPAC_T67 | 533-536 | "http://rdf.glycoinfo.org/glycan/G70440ZO" | denotes | all |
| GlycanIUPAC_T68 | 533-536 | "http://rdf.glycoinfo.org/glycan/G29951RR" | denotes | all |
| GlycanIUPAC_T69 | 533-536 | "http://rdf.glycoinfo.org/glycan/G58402TI" | denotes | all |
| GlycanIUPAC_T70 | 533-536 | "http://rdf.glycoinfo.org/glycan/G39875TP" | denotes | all |
| GlycanIUPAC_T71 | 533-536 | "http://rdf.glycoinfo.org/glycan/G83439QV" | denotes | all |
| GlycanIUPAC_T72 | 533-536 | "http://rdf.glycoinfo.org/glycan/G41762RC" | denotes | all |
| GlycanIUPAC_T73 | 533-536 | "http://rdf.glycoinfo.org/glycan/G91604UI" | denotes | all |
| GlycanIUPAC_T74 | 533-536 | "http://rdf.glycoinfo.org/glycan/G88447WE" | denotes | all |
| GlycanIUPAC_T75 | 533-536 | "http://rdf.glycoinfo.org/glycan/G93634BS" | denotes | all |
| GlycanIUPAC_T76 | 533-536 | "http://rdf.glycoinfo.org/glycan/G02587BH" | denotes | all |
| GlycanIUPAC_T77 | 533-536 | "http://rdf.glycoinfo.org/glycan/G43511MX" | denotes | all |
| GlycanIUPAC_T78 | 533-536 | "http://rdf.glycoinfo.org/glycan/G64958DH" | denotes | all |
| GlycanIUPAC_T79 | 533-536 | "http://rdf.glycoinfo.org/glycan/G30384TR" | denotes | all |
| GlycanIUPAC_T80 | 533-536 | "http://rdf.glycoinfo.org/glycan/G15624EX" | denotes | all |
| GlycanIUPAC_T81 | 533-536 | "http://rdf.glycoinfo.org/glycan/G22706ST" | denotes | all |
| GlycanIUPAC_T82 | 533-536 | "http://rdf.glycoinfo.org/glycan/G57408PI" | denotes | all |
| GlycanIUPAC_T83 | 533-536 | "http://rdf.glycoinfo.org/glycan/G86403XX" | denotes | all |
| GlycanIUPAC_T84 | 533-536 | "http://rdf.glycoinfo.org/glycan/G78043YB" | denotes | all |
| GlycanIUPAC_T85 | 533-536 | "http://rdf.glycoinfo.org/glycan/G18952JK" | denotes | all |
| GlycanIUPAC_T86 | 533-536 | "http://rdf.glycoinfo.org/glycan/G49020ND" | denotes | all |
| GlycanIUPAC_T87 | 533-536 | "http://rdf.glycoinfo.org/glycan/G63590YW" | denotes | all |
| GlycanIUPAC_T88 | 533-536 | "http://rdf.glycoinfo.org/glycan/G22793KS" | denotes | all |
| GlycanIUPAC_T89 | 533-536 | "http://rdf.glycoinfo.org/glycan/G64134SS" | denotes | all |
| GlycanIUPAC_T90 | 533-536 | "http://rdf.glycoinfo.org/glycan/G17338HY" | denotes | all |
| GlycanIUPAC_T91 | 533-536 | "http://rdf.glycoinfo.org/glycan/G99745XF" | denotes | all |
| GlycanIUPAC_T92 | 533-536 | "http://rdf.glycoinfo.org/glycan/G27782HN" | denotes | all |
| GlycanIUPAC_T93 | 533-536 | "http://rdf.glycoinfo.org/glycan/G57496DC" | denotes | all |
| GlycanIUPAC_T94 | 533-536 | "http://rdf.glycoinfo.org/glycan/G93169WB" | denotes | all |
| GlycanIUPAC_T95 | 533-536 | "http://rdf.glycoinfo.org/glycan/G05518TD" | denotes | all |
| GlycanIUPAC_T96 | 533-536 | "http://rdf.glycoinfo.org/glycan/G62603DN" | denotes | all |
| GlycanIUPAC_T97 | 533-536 | "http://rdf.glycoinfo.org/glycan/G59574FS" | denotes | all |
| GlycanIUPAC_T98 | 533-536 | "http://rdf.glycoinfo.org/glycan/G47567WC" | denotes | all |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 172-188 | Glycan | denotes | lactosylceramide | https://api.glycosmos.org/wurcs2image/latest/png/binary/G84224TW |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 172-188 | Glycan | denotes | lactosylceramide | https://api.glycosmos.org/wurcs2image/latest/png/binary/G84224TW |
GlyCosmos15-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 338-343 | Body_part | denotes | ovary | http://purl.obolibrary.org/obo/UBERON_0000992 |
GlyCosmos15-Taxon
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 250-255 | Organism | denotes | mouse | 10088|10090 |
| T3 | 322-337 | Organism | denotes | Chinese hamster | 10029 |
| T4 | 1150-1156 | Organism | denotes | medaka | 8090 |
| T5 | 1160-1169 | Organism | denotes | tetraodon | 47144 |
| T6 | 1194-1199 | Organism | denotes | human | 9606 |
| T7 | 1228-1237 | Organism | denotes | zebrafish | 7955 |
| T8 | 1764-1780 | Organism | denotes | Escherichia coli | 562 |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-114 | Sentence | denotes | Substitution of the N-glycan function in glycosyltransferases by specific amino acids: ST3Gal-V as a model enzyme. |
| T2 | 115-189 | Sentence | denotes | The sialyltranferase ST3Gal-V transfers a sialic acid to lactosylceramide. |
| T3 | 190-402 | Sentence | denotes | We investigated the role of each of the N-glycans modifying mouse ST3Gal-V (mST3Gal-V) by measuring the in vitro enzyme activity of Chinese hamster ovary (CHO) cells transfected with ST3Gal-V cDNA or its mutants. |
| T4 | 403-623 | Sentence | denotes | By examining mutants of mST3Gal-V, in which each asparagine was replaced with glutamine (N180Q, N224Q, N334Q), we determined that all three sites are N-glycosylated and that each N-glycan is required for enzyme activity. |
| T5 | 624-716 | Sentence | denotes | Despite their importance, N-glycosylation sites in ST3Gal-V are not conserved among species. |
| T6 | 717-1036 | Sentence | denotes | Therefore, we considered whether the function in the activity that is performed in mST3Gal-V by the N-glycan could be substituted for by specific amino acid residues selected from the ST3Gal-V of other species or from related sialyltransferases (ST3Gal-I, -II, -III, and -IV), placed at or near the glycosylation sites. |
| T7 | 1037-1244 | Sentence | denotes | To this end, we constructed a series of interspecies mutants for mST3Gal-V, specifically, mST3Gal-V-H177D-N180S (medaka or tetraodon type), mST3Gal-V-N224K (human type), and mST3Gal-V-T336Q (zebrafish type). |
| T8 | 1245-1334 | Sentence | denotes | The ST3Gal-V activity of these mutants was quite similar to that of the wild-type enzyme. |
| T9 | 1335-1523 | Sentence | denotes | Thus, we have demonstrated here that the N-glycans on mST3Gal-V are required for activity but can be substituted for specific amino acid residues placed at or near the glycosylation sites. |
| T10 | 1524-1636 | Sentence | denotes | We named this method SUNGA (substitution of N-glycan functions in glycosyltransferases by specific amino acids). |
| T11 | 1637-1884 | Sentence | denotes | Furthermore, we verified that the ST3Gal-V mutant created using the SUNGA method maintains its high activity when expressed in Escherichia coli thereby establishing the usefulness of the SUNGA method in exploring the function of N-glycans in vivo. |
GlyCosmos15-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 172-188 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | lactosylceramide | http://www.glycoepitope.jp/epitopes/EP0078 |
GlyCosmos15-FMA
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 338-343 | Body_part | denotes | ovary | FMA:7209 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 250-255 | OrganismTaxon | denotes | mouse | 10088|10090 |
| T3 | 322-337 | OrganismTaxon | denotes | Chinese hamster | 10029 |
| T4 | 1150-1156 | OrganismTaxon | denotes | medaka | 8090 |
| T5 | 1160-1169 | OrganismTaxon | denotes | tetraodon | 47144 |
| T6 | 1194-1199 | OrganismTaxon | denotes | human | 9606 |
| T7 | 1228-1237 | OrganismTaxon | denotes | zebrafish | 7955 |
| T8 | 1764-1780 | OrganismTaxon | denotes | Escherichia coli | 562 |
GlyCosmos-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 172-188 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | lactosylceramide | http://www.glycoepitope.jp/epitopes/EP0078 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 338-343 | Body_part | denotes | ovary | http://purl.obolibrary.org/obo/UBERON_0000992 |