PubMed:16291231 JSONTXT

{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":607,"end":620},"obj":"HP_0001427"}],"text":"Protein nitration in biological aging: proteomic and tandem mass spectrometric characterization of nitrated sites.\nProteomic techniques for the identification of 3-nitrotyrosine-containing proteins in various biological systems are described with emphasis on the direct mass spectrometric detection and sequencing of 3-nitrotyrosine-containing peptides. Strengths and weaknesses of various separation and mass spectrometric techniques are discussed. Some examples for the MS/MS analysis of nitrated peptides obtained from aging rat heart and skeletal muscle are provided, such as nitration of Tyr105 of the mitochondrial electron-transfer flavoprotein and Tyr14 of creatine kinase."}