| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-145 |
Sentence |
denotes |
Molecular characterisation of soybean polysaccharides: an approach by size exclusion chromatography, dynamic and static light scattering methods. |
| TextSentencer_T2 |
146-276 |
Sentence |
denotes |
Water soluble polysaccharides from soybean (SSPS) have a pectin-like structure and are used as stabilisers in acidified beverages. |
| TextSentencer_T3 |
277-426 |
Sentence |
denotes |
Physicochemical properties such as structure, molecular weight and shape or conformation are primary factors controlling their functional properties. |
| TextSentencer_T4 |
427-648 |
Sentence |
denotes |
Two soybean polysaccharides, a native SSPS and a modified SSPS treated with beta-(1-->4)-D-galactosidase (GPase/SSPS) were studied by dynamic and static light scattering (DLS, SLS) and size exclusion chromatography (SEC). |
| TextSentencer_T5 |
649-904 |
Sentence |
denotes |
Consecutive filtrations using a range of membrane pore size removed a small fraction of macromolecular aggregates from dilute polysaccharide solutions with relatively little effect on the major component molecules as monitored by DLS and SEC measurements. |
| TextSentencer_T6 |
905-1030 |
Sentence |
denotes |
Access to aggregate-free dilute solutions of SSPS and GPase/SSPS allowed the direct measurement of molecular characteristics. |
| TextSentencer_T7 |
1031-1172 |
Sentence |
denotes |
SLS results showed that SSPS had a weight average molecular weight of (645+/-11)x 10(3)g/mol and a radius of gyration, Rg, of (23.5+/-2.8)nm. |
| TextSentencer_T8 |
1173-1403 |
Sentence |
denotes |
By comparing R(g) with the hydrodynamic radius, Rh (21.1+/-0.5 nm) obtained from DLS, the structural parameter rho (Rg/Rh) was found to be 1.1, suggesting that SSPS has an overall globular shape due to a highly branched structure. |
| TextSentencer_T9 |
1404-1537 |
Sentence |
denotes |
The modified SSPS had a significantly lower molecular weight (287+/-18)x 10(3)g/mol but a similar radius of gyration (23.2+/-1.7 nm). |
| TextSentencer_T10 |
1538-1658 |
Sentence |
denotes |
The structure parameter rho of GPase/SSPS was higher (rho=1.3) because of a smaller hydrodynamic radius (17.7+/-1.8 nm). |
| TextSentencer_T11 |
1659-1809 |
Sentence |
denotes |
This suggests that GPase/SSPS has a much less branched structure yet still differs significantly from a linear random coil conformation (rho=1.7-2.0). |
| TextSentencer_T12 |
1810-2024 |
Sentence |
denotes |
The results derived from SLS and DLS are in agreement with the conclusions obtained from a chemical analysis where the reduction of molecular weight of GPase/SSPS was caused by the cleavage of galactan side chains. |
| T1 |
0-145 |
Sentence |
denotes |
Molecular characterisation of soybean polysaccharides: an approach by size exclusion chromatography, dynamic and static light scattering methods. |
| T2 |
146-276 |
Sentence |
denotes |
Water soluble polysaccharides from soybean (SSPS) have a pectin-like structure and are used as stabilisers in acidified beverages. |
| T3 |
277-426 |
Sentence |
denotes |
Physicochemical properties such as structure, molecular weight and shape or conformation are primary factors controlling their functional properties. |
| T4 |
427-648 |
Sentence |
denotes |
Two soybean polysaccharides, a native SSPS and a modified SSPS treated with beta-(1-->4)-D-galactosidase (GPase/SSPS) were studied by dynamic and static light scattering (DLS, SLS) and size exclusion chromatography (SEC). |
| T5 |
649-904 |
Sentence |
denotes |
Consecutive filtrations using a range of membrane pore size removed a small fraction of macromolecular aggregates from dilute polysaccharide solutions with relatively little effect on the major component molecules as monitored by DLS and SEC measurements. |
| T6 |
905-1030 |
Sentence |
denotes |
Access to aggregate-free dilute solutions of SSPS and GPase/SSPS allowed the direct measurement of molecular characteristics. |
| T7 |
1031-1172 |
Sentence |
denotes |
SLS results showed that SSPS had a weight average molecular weight of (645+/-11)x 10(3)g/mol and a radius of gyration, Rg, of (23.5+/-2.8)nm. |
| T8 |
1173-1403 |
Sentence |
denotes |
By comparing R(g) with the hydrodynamic radius, Rh (21.1+/-0.5 nm) obtained from DLS, the structural parameter rho (Rg/Rh) was found to be 1.1, suggesting that SSPS has an overall globular shape due to a highly branched structure. |
| T9 |
1404-1537 |
Sentence |
denotes |
The modified SSPS had a significantly lower molecular weight (287+/-18)x 10(3)g/mol but a similar radius of gyration (23.2+/-1.7 nm). |
| T10 |
1538-1658 |
Sentence |
denotes |
The structure parameter rho of GPase/SSPS was higher (rho=1.3) because of a smaller hydrodynamic radius (17.7+/-1.8 nm). |
| T11 |
1659-1809 |
Sentence |
denotes |
This suggests that GPase/SSPS has a much less branched structure yet still differs significantly from a linear random coil conformation (rho=1.7-2.0). |
| T12 |
1810-2024 |
Sentence |
denotes |
The results derived from SLS and DLS are in agreement with the conclusions obtained from a chemical analysis where the reduction of molecular weight of GPase/SSPS was caused by the cleavage of galactan side chains. |
