PubMed:16192264
Annnotations
GlyCosmos6-Glycan-Motif-Image
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 36-47 | Glycan_Motif | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT |
| T2 | 237-248 | Glycan_Motif | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT |
| T3 | 384-405 | Glycan_Motif | denotes | N-acetylgalactosamine | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G27025MB |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-89 | Sentence | denotes | Recognition of sulfation pattern of chondroitin sulfate by uronosyl 2-O-sulfotransferase. |
| T2 | 90-437 | Sentence | denotes | We have shown previously that a highly sulfated sequence, GalNAc(4,6-SO(4))-GlcA(2SO(4))-GalNAc(6SO(4)), is present at the nonreducing terminal of chondroitin sulfate (CS), and this structure was synthesized from a unique sequence, GalNAc(4SO(4))-GlcA(2SO(4))-GalNAc(6SO(4)), by sulfation with N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase. |
| T3 | 438-777 | Sentence | denotes | Uronosyl 2-O-sulfotrasferase (2OST), which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 2 of the GlcA residue of CS, is expected to be involved in synthesis of these structures; however, the specificity of 2OST concerning recognition of the sulfation pattern of the acceptor has largely remained unclear. |
| T4 | 778-919 | Sentence | denotes | In the present study, we examined the specificity of 2OST in terms of recognition of the sulfation pattern around the targeting GlcA residue. |
| T5 | 920-999 | Sentence | denotes | The recombinant 2OST could sulfate CS-A, CS-C, and desulfated dermatan sulfate. |
| T6 | 1000-1255 | Sentence | denotes | When [(35)S]glycosaminoglycans formed from CS-A after the reaction with the recombinant 2OST and [(35)S]PAPS were subjected to limited digestion with chondroitinase ACII, a radioactive tetrasaccharide (Tetra A) was obtained as a sole intermediate product. |
| T7 | 1256-1385 | Sentence | denotes | The sequence of Tetra A was found to be DeltaHexA-GalNAc(4SO(4))-GlcA(2SO(4))-GalNAc(6SO(4)) by enzymatic and chemical reactions. |
| T8 | 1386-1544 | Sentence | denotes | These observations indicate that 2OST transfers sulfate preferentially to the GlcA residue located in a unique sequence, -GalNAc(4SO(4))-GlcA-GalNAc(6SO(4))-. |
| T9 | 1545-1796 | Sentence | denotes | When oligosaccharides with different sulfation patterns were used as the acceptor, GalNAc(4SO(4))-GlcA-GalNAc(6SO(4)) and GlcA-GalNAc(4SO(4))-GlcA-GalNAc(6SO(4)) were the best acceptors for 2OST among trisaccharides and tetrasaccharides, respectively. |
| T10 | 1797-1909 | Sentence | denotes | These results suggest that 2OST may be involved in the synthesis of the highly sulfated structure found in CS-A. |
GlyCosmos6-Glycan-Motif-Structure
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 36-47 | https://glytoucan.org/Structures/Glycans/G43702JT | denotes | chondroitin |
| T2 | 237-248 | https://glytoucan.org/Structures/Glycans/G43702JT | denotes | chondroitin |
| T3 | 384-405 | https://glytoucan.org/Structures/Glycans/G27025MB | denotes | N-acetylgalactosamine |
Glycosmos6-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glyco_epitope_db_id |
|---|---|---|---|---|---|
| T1 | 36-47 | GlycoEpitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T2 | 237-248 | GlycoEpitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
mondo_disease
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T1 | 955-959 | Disease | denotes | CS-A | http://purl.obolibrary.org/obo/MONDO_0019569 |
| T2 | 961-965 | Disease | denotes | CS-C | http://purl.obolibrary.org/obo/MONDO_0018616 |
| T3 | 1043-1047 | Disease | denotes | CS-A | http://purl.obolibrary.org/obo/MONDO_0019569 |
| T4 | 1904-1908 | Disease | denotes | CS-A | http://purl.obolibrary.org/obo/MONDO_0019569 |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 36-47 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T2 | 148-154 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T3 | 179-185 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T4 | 237-248 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T5 | 322-328 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T6 | 350-356 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T7 | 1306-1312 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T8 | 1334-1340 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T9 | 1508-1514 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T10 | 1528-1534 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T11 | 1628-1634 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T12 | 1648-1654 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T13 | 1672-1678 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T14 | 1692-1698 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
GlyCosmos-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 36-47 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T2 | 237-248 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
GlyCosmos15-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T1 | 955-959 | Disease | denotes | CS-A | http://purl.obolibrary.org/obo/MONDO_0019569 |
| T2 | 961-965 | Disease | denotes | CS-C | http://purl.obolibrary.org/obo/MONDO_0018616 |
| T3 | 1043-1047 | Disease | denotes | CS-A | http://purl.obolibrary.org/obo/MONDO_0019569 |
| T4 | 1904-1908 | Disease | denotes | CS-A | http://purl.obolibrary.org/obo/MONDO_0019569 |
GlyCosmos15-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 1229-1233 | Body_part | denotes | sole | http://purl.obolibrary.org/obo/UBERON_0008338 |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-89 | Sentence | denotes | Recognition of sulfation pattern of chondroitin sulfate by uronosyl 2-O-sulfotransferase. |
| T2 | 90-437 | Sentence | denotes | We have shown previously that a highly sulfated sequence, GalNAc(4,6-SO(4))-GlcA(2SO(4))-GalNAc(6SO(4)), is present at the nonreducing terminal of chondroitin sulfate (CS), and this structure was synthesized from a unique sequence, GalNAc(4SO(4))-GlcA(2SO(4))-GalNAc(6SO(4)), by sulfation with N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase. |
| T3 | 438-777 | Sentence | denotes | Uronosyl 2-O-sulfotrasferase (2OST), which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 2 of the GlcA residue of CS, is expected to be involved in synthesis of these structures; however, the specificity of 2OST concerning recognition of the sulfation pattern of the acceptor has largely remained unclear. |
| T4 | 778-919 | Sentence | denotes | In the present study, we examined the specificity of 2OST in terms of recognition of the sulfation pattern around the targeting GlcA residue. |
| T5 | 920-999 | Sentence | denotes | The recombinant 2OST could sulfate CS-A, CS-C, and desulfated dermatan sulfate. |
| T6 | 1000-1255 | Sentence | denotes | When [(35)S]glycosaminoglycans formed from CS-A after the reaction with the recombinant 2OST and [(35)S]PAPS were subjected to limited digestion with chondroitinase ACII, a radioactive tetrasaccharide (Tetra A) was obtained as a sole intermediate product. |
| T7 | 1256-1385 | Sentence | denotes | The sequence of Tetra A was found to be DeltaHexA-GalNAc(4SO(4))-GlcA(2SO(4))-GalNAc(6SO(4)) by enzymatic and chemical reactions. |
| T8 | 1386-1544 | Sentence | denotes | These observations indicate that 2OST transfers sulfate preferentially to the GlcA residue located in a unique sequence, -GalNAc(4SO(4))-GlcA-GalNAc(6SO(4))-. |
| T9 | 1545-1796 | Sentence | denotes | When oligosaccharides with different sulfation patterns were used as the acceptor, GalNAc(4SO(4))-GlcA-GalNAc(6SO(4)) and GlcA-GalNAc(4SO(4))-GlcA-GalNAc(6SO(4)) were the best acceptors for 2OST among trisaccharides and tetrasaccharides, respectively. |
| T10 | 1797-1909 | Sentence | denotes | These results suggest that 2OST may be involved in the synthesis of the highly sulfated structure found in CS-A. |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-89 | Sentence | denotes | Recognition of sulfation pattern of chondroitin sulfate by uronosyl 2-O-sulfotransferase. |
| T2 | 90-437 | Sentence | denotes | We have shown previously that a highly sulfated sequence, GalNAc(4,6-SO(4))-GlcA(2SO(4))-GalNAc(6SO(4)), is present at the nonreducing terminal of chondroitin sulfate (CS), and this structure was synthesized from a unique sequence, GalNAc(4SO(4))-GlcA(2SO(4))-GalNAc(6SO(4)), by sulfation with N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase. |
| T3 | 438-777 | Sentence | denotes | Uronosyl 2-O-sulfotrasferase (2OST), which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 2 of the GlcA residue of CS, is expected to be involved in synthesis of these structures; however, the specificity of 2OST concerning recognition of the sulfation pattern of the acceptor has largely remained unclear. |
| T4 | 778-919 | Sentence | denotes | In the present study, we examined the specificity of 2OST in terms of recognition of the sulfation pattern around the targeting GlcA residue. |
| T5 | 920-999 | Sentence | denotes | The recombinant 2OST could sulfate CS-A, CS-C, and desulfated dermatan sulfate. |
| T6 | 1000-1255 | Sentence | denotes | When [(35)S]glycosaminoglycans formed from CS-A after the reaction with the recombinant 2OST and [(35)S]PAPS were subjected to limited digestion with chondroitinase ACII, a radioactive tetrasaccharide (Tetra A) was obtained as a sole intermediate product. |
| T7 | 1256-1385 | Sentence | denotes | The sequence of Tetra A was found to be DeltaHexA-GalNAc(4SO(4))-GlcA(2SO(4))-GalNAc(6SO(4)) by enzymatic and chemical reactions. |
| T8 | 1386-1544 | Sentence | denotes | These observations indicate that 2OST transfers sulfate preferentially to the GlcA residue located in a unique sequence, -GalNAc(4SO(4))-GlcA-GalNAc(6SO(4))-. |
| T9 | 1545-1796 | Sentence | denotes | When oligosaccharides with different sulfation patterns were used as the acceptor, GalNAc(4SO(4))-GlcA-GalNAc(6SO(4)) and GlcA-GalNAc(4SO(4))-GlcA-GalNAc(6SO(4)) were the best acceptors for 2OST among trisaccharides and tetrasaccharides, respectively. |
| T10 | 1797-1909 | Sentence | denotes | These results suggest that 2OST may be involved in the synthesis of the highly sulfated structure found in CS-A. |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 36-47 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T2 | 148-154 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T3 | 179-185 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T4 | 237-248 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T5 | 322-328 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T6 | 350-356 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T7 | 1306-1312 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T8 | 1334-1340 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T9 | 1508-1514 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T10 | 1528-1534 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T11 | 1628-1634 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T12 | 1648-1654 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T13 | 1672-1678 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T14 | 1692-1698 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
GlyCosmos15-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 36-47 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T2 | 237-248 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 1229-1233 | Body_part | denotes | sole | http://purl.obolibrary.org/obo/UBERON_0008338 |