PubMed:1616933
Annnotations
CL-cell
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 0-16 | Cell | denotes | Endothelial cell | http://purl.obolibrary.org/obo/CL:0000115 |
| T2 | 94-122 | Cell | denotes | vascular smooth muscle cells | http://purl.obolibrary.org/obo/CL:0000359 |
| T3 | 103-122 | Cell | denotes | smooth muscle cells | http://purl.obolibrary.org/obo/CL:0000192 |
| T4 | 156-172 | Cell | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL:0000115 |
| T5 | 234-253 | Cell | denotes | smooth muscle cells | http://purl.obolibrary.org/obo/CL:0000192 |
| T6 | 414-431 | Cell | denotes | endothelial cells | http://purl.obolibrary.org/obo/CL:0000115 |
| T7 | 581-597 | Cell | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL:0000115 |
| T8 | 854-870 | Cell | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL:0000115 |
| T9 | 1115-1131 | Cell | denotes | Endothelial cell | http://purl.obolibrary.org/obo/CL:0000115 |
| T10 | 1555-1574 | Cell | denotes | smooth muscle cells | http://purl.obolibrary.org/obo/CL:0000192 |
| T11 | 2093-2104 | Cell | denotes | fractions A | http://purl.obolibrary.org/obo/CL:0002045 |
| T12 | 2191-2207 | Cell | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL:0000115 |
| T13 | 2286-2296 | Cell | denotes | fraction A | http://purl.obolibrary.org/obo/CL:0002045 |
| T14 | 2337-2347 | Cell | denotes | fraction B | http://purl.obolibrary.org/obo/CL:0002047 |
| T15 | 2498-2514 | Cell | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL:0000115 |
Anatomy-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 103-116 | Body_part | denotes | smooth muscle | http://purl.obolibrary.org/obo/MAT_0000303 |
| T2 | 228-233 | Body_part | denotes | aorta | http://purl.obolibrary.org/obo/MAT_0000035 |
| T3 | 234-247 | Body_part | denotes | smooth muscle | http://purl.obolibrary.org/obo/MAT_0000303 |
| T4 | 1555-1568 | Body_part | denotes | smooth muscle | http://purl.obolibrary.org/obo/MAT_0000303 |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 1774-1785 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T2 | 1812-1823 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T3 | 1923-1934 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
GlyCosmos-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 1774-1785 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T2 | 1812-1823 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T3 | 1923-1934 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
GlyCosmos15-NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 149-155 | OrganismTaxon | denotes | bovine | 9913 |
| T2 | 221-227 | OrganismTaxon | denotes | bovine | 9913 |
GlyCosmos15-CL
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 0-16 | Cell | denotes | Endothelial cell | http://purl.obolibrary.org/obo/CL:0000115 |
| T2 | 94-122 | Cell | denotes | vascular smooth muscle cells | http://purl.obolibrary.org/obo/CL:0000359 |
| T3 | 103-122 | Cell | denotes | smooth muscle cells | http://purl.obolibrary.org/obo/CL:0000192 |
| T4 | 156-172 | Cell | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL:0000115 |
| T5 | 234-253 | Cell | denotes | smooth muscle cells | http://purl.obolibrary.org/obo/CL:0000192 |
| T6 | 414-431 | Cell | denotes | endothelial cells | http://purl.obolibrary.org/obo/CL:0000115 |
| T7 | 581-597 | Cell | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL:0000115 |
| T8 | 854-870 | Cell | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL:0000115 |
| T9 | 1115-1131 | Cell | denotes | Endothelial cell | http://purl.obolibrary.org/obo/CL:0000115 |
| T10 | 1555-1574 | Cell | denotes | smooth muscle cells | http://purl.obolibrary.org/obo/CL:0000192 |
| T11 | 2093-2104 | Cell | denotes | fractions A | http://purl.obolibrary.org/obo/CL:0002045 |
| T12 | 2191-2207 | Cell | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL:0000115 |
| T13 | 2286-2296 | Cell | denotes | fraction A | http://purl.obolibrary.org/obo/CL:0002045 |
| T14 | 2337-2347 | Cell | denotes | fraction B | http://purl.obolibrary.org/obo/CL:0002047 |
| T15 | 2498-2514 | Cell | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL:0000115 |
GlyCosmos15-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 0-16 | Body_part | denotes | Endothelial cell | http://purl.obolibrary.org/obo/CL_0000115 |
| T2 | 94-122 | Body_part | denotes | vascular smooth muscle cells | http://purl.obolibrary.org/obo/CL_0000359 |
| T3 | 156-172 | Body_part | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL_0000115 |
| T4 | 228-247 | Body_part | denotes | aorta smooth muscle | http://purl.obolibrary.org/obo/UBERON_0004178 |
| T5 | 414-431 | Body_part | denotes | endothelial cells | http://purl.obolibrary.org/obo/CL_0000115 |
| T6 | 527-537 | Body_part | denotes | cell layer | http://purl.obolibrary.org/obo/UBERON_0000119 |
| T7 | 581-597 | Body_part | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL_0000115 |
| T8 | 695-700 | Body_part | denotes | layer | http://purl.obolibrary.org/obo/UBERON_0000119|http://purl.obolibrary.org/obo/UBERON_0022303 |
| T10 | 854-870 | Body_part | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL_0000115 |
| T11 | 1115-1131 | Body_part | denotes | Endothelial cell | http://purl.obolibrary.org/obo/CL_0000115 |
| T12 | 1555-1574 | Body_part | denotes | smooth muscle cells | http://purl.obolibrary.org/obo/CL_0000192 |
| T13 | 2093-2104 | Body_part | denotes | fractions A | http://purl.obolibrary.org/obo/CL_0002045 |
| T14 | 2191-2207 | Body_part | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL_0000115 |
| T15 | 2286-2296 | Body_part | denotes | fraction A | http://purl.obolibrary.org/obo/CL_0002045 |
| T16 | 2337-2347 | Body_part | denotes | fraction B | http://purl.obolibrary.org/obo/CL_0002047 |
| T17 | 2498-2514 | Body_part | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL_0000115 |
GlyCosmos15-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 103-116 | Body_part | denotes | smooth muscle | http://purl.obolibrary.org/obo/MAT_0000303 |
| T2 | 228-233 | Body_part | denotes | aorta | http://purl.obolibrary.org/obo/MAT_0000035 |
| T3 | 234-247 | Body_part | denotes | smooth muscle | http://purl.obolibrary.org/obo/MAT_0000303 |
| T4 | 1555-1568 | Body_part | denotes | smooth muscle | http://purl.obolibrary.org/obo/MAT_0000303 |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-123 | Sentence | denotes | Endothelial cell-conditioned medium modulates the synthesis and structure of proteoglycans in vascular smooth muscle cells. |
| T2 | 124-254 | Sentence | denotes | We studied the effect of bovine endothelial cell-conditioned medium on proteoglycan synthesis by bovine aorta smooth muscle cells. |
| T3 | 255-432 | Sentence | denotes | Confluent cultures were incubated with [35S]sulfate, [3H]glucosamine or [3H]serine in medium alone (control), or medium that had been conditioned on confluent endothelial cells. |
| T4 | 433-554 | Sentence | denotes | Metabolically labelled proteoglycans secreted into the culture medium and associated with the cell layer were quantified. |
| T5 | 555-839 | Sentence | denotes | During a 24 h incubation, endothelial cell-conditioned medium increased [35S]sulfate and [3H]glucosamine incorporation into medium and cell-layer proteoglycans by 59% and 95%, respectively, above controls. [3H]Serine incorporation into proteoglycan core protein was increased by 150%. |
| T6 | 840-948 | Sentence | denotes | The effect of endothelial cell-conditioned medium on [35S]sulfate incorporation was concentration dependent. |
| T7 | 949-1114 | Sentence | denotes | The stimulatory effects of the conditioned medium were abolished by cycloheximide and actinomycin D, inhibitors of protein synthesis and transcription, respectively. |
| T8 | 1115-1315 | Sentence | denotes | Endothelial cell-conditioned medium caused no significant change in the degradation or secretion of proteoglycans, indicating that the increase in proteoglycans was due to increased de novo synthesis. |
| T9 | 1316-1479 | Sentence | denotes | TGF-beta neutralizing antibody inhibited 22% of the stimulatory effect of the conditioned medium, suggesting that part of the stimulation was mediated by TGF-beta. |
| T10 | 1480-1665 | Sentence | denotes | Ion-exchange chromatography of [35S]proteoglycans in the culture medium of smooth muscle cells yielded two major peaks at 0.52 and 0.57 M NaCl in both control and experimental cultures. |
| T11 | 1666-1722 | Sentence | denotes | In both cases the second peak, which represented approx. |
| T12 | 1723-1909 | Sentence | denotes | 80% of the total radioactivity, contained isomeric chondroitin sulfate proteoglycan with chondroitin sulfate and dermatan sulfate accounting for 90% and 10% of the isomers, respectively. |
| T13 | 1910-2056 | Sentence | denotes | The isomeric chondroitin sulfate proteoglycan was fractionated by hydrodynamic size on Sepharose CL-4B, resulting in three fractions (A, B and C). |
| T14 | 2057-2381 | Sentence | denotes | Analytical column chromatography of fractions A and B on Sepharose CL-2B demonstrated that proteoglycans from cultures incubated with endothelial cell-conditioned medium were larger in size than those from control cultures (M(r) fraction A, 1700,000, compared with 1200,000 M(r); fraction B, 540,000, compared with 390,000). |
| T15 | 2382-2440 | Sentence | denotes | The molecular weights of the core proteins were unchanged. |
| T16 | 2441-2673 | Sentence | denotes | The larger size of proteoglycan A in cultures exposed to endothelial cell-conditioned medium was due to an increase in both the glycosaminoglycan chain number (29 compared to 25) and molecular mass (M(r) 52,000, compared to 40,000). |
| T17 | 2674-2805 | Sentence | denotes | The hydrodynamic size of the glycosaminoglycans in proteoglycan B of control and experimental cultures was identical (M(r) 40,000). |
| T18 | 2806-2991 | Sentence | denotes | Therefore, the increase in the molecular mass of this proteoglycan was attributable to an increase in glycosaminoglycan chain number (12 compared to 9).(ABSTRACT TRUNCATED AT 400 WORDS) |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 1774-1785 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T2 | 1812-1823 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T3 | 1923-1934 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
GlyCosmos15-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 1774-1785 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T2 | 1812-1823 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T3 | 1923-1934 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 149-155 | OrganismTaxon | denotes | bovine | 9913 |
| T2 | 221-227 | OrganismTaxon | denotes | bovine | 9913 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 0-16 | Body_part | denotes | Endothelial cell | http://purl.obolibrary.org/obo/CL_0000115 |
| T2 | 94-122 | Body_part | denotes | vascular smooth muscle cells | http://purl.obolibrary.org/obo/CL_0000359 |
| T3 | 156-172 | Body_part | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL_0000115 |
| T4 | 228-247 | Body_part | denotes | aorta smooth muscle | http://purl.obolibrary.org/obo/UBERON_0004178 |
| T5 | 414-431 | Body_part | denotes | endothelial cells | http://purl.obolibrary.org/obo/CL_0000115 |
| T6 | 527-537 | Body_part | denotes | cell layer | http://purl.obolibrary.org/obo/UBERON_0000119 |
| T7 | 581-597 | Body_part | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL_0000115 |
| T8 | 695-700 | Body_part | denotes | layer | http://purl.obolibrary.org/obo/UBERON_0000119|http://purl.obolibrary.org/obo/UBERON_0022303 |
| T10 | 854-870 | Body_part | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL_0000115 |
| T11 | 1115-1131 | Body_part | denotes | Endothelial cell | http://purl.obolibrary.org/obo/CL_0000115 |
| T12 | 1555-1574 | Body_part | denotes | smooth muscle cells | http://purl.obolibrary.org/obo/CL_0000192 |
| T13 | 2093-2104 | Body_part | denotes | fractions A | http://purl.obolibrary.org/obo/CL_0002045 |
| T14 | 2191-2207 | Body_part | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL_0000115 |
| T15 | 2286-2296 | Body_part | denotes | fraction A | http://purl.obolibrary.org/obo/CL_0002045 |
| T16 | 2337-2347 | Body_part | denotes | fraction B | http://purl.obolibrary.org/obo/CL_0002047 |
| T17 | 2498-2514 | Body_part | denotes | endothelial cell | http://purl.obolibrary.org/obo/CL_0000115 |