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PubMed:16131178 JSONTXT

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GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 179-186 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T2 564-571 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T3 604-611 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T4 731-738 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T5 1093-1100 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T6 1140-1147 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T7 1259-1266 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T8 1300-1307 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T9 1370-1377 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T10 1499-1506 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T11 1780-1787 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 179-186 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T2 564-571 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T3 604-611 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T4 731-738 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T5 1093-1100 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T6 1140-1147 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T7 1259-1266 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T8 1300-1307 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T9 1370-1377 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T10 1499-1506 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT
T11 1780-1787 Glycan denotes maltose https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-78 Sentence denotes A modular nanoparticle-based system for reagentless small molecule biosensing.
T2 79-198 Sentence denotes Metalloprotein tethered CdSe nanoparticles have been generated to provide selective and reagentless maltose biosensing.
T3 199-398 Sentence denotes As opposed to cell or protein detection by semiconducting nanoparticle bioconjugates, a modular method for small-molecule detection using semiconducting nanoparticle bioconjugates has been difficult.
T4 399-492 Sentence denotes Here we report a method for reagentless protein-based semiconducting nanoparticle biosensors.
T5 493-691 Sentence denotes This method uses Ru(II) complex-CdSe nanoparticle interactions and the maltose-induced conformation changes of maltose binding protein to alter the CdSe nanoparticle fluorescence emission intensity.
T6 692-876 Sentence denotes In this proof-of-principle system, the maltose-induced protein conformation changes alter the Ru(II) complex-CdSe nanoparticle interaction, which increases the CdSe emission intensity.
T7 877-1045 Sentence denotes Altered CdSe emission intensity effects are best described as electron transfer from the Ru(II) complex to the CdSe excited state forming the nonfluorescent CdSe anion.
T8 1046-1198 Sentence denotes Four surface-cysteine, Ru(II) complex-attached maltose-binding proteins have been studied for maltose dependent alteration of CdSe emission intensities.
T9 1199-1416 Sentence denotes With 3.0-3.5 nm diameter CdSe nanoparticles, all ruthenated maltose-binding proteins display similar maltose-dependent increases (1.4-fold) in CdSe emission intensity and maltose binding affinities (KA = 3 x 106 M-1).
T10 1417-1527 Sentence denotes For these four systems, the only difference was the sample-to-sample variation in maltose-dependent responses.
T11 1528-1681 Sentence denotes Thus, very few surface cysteine mutations need to be examined to find a successful biosensor, as opposed to analogous systems using organic fluorophores.
T12 1682-1873 Sentence denotes This strategy generates a unimolecular, or reagentless, semiconducting nanoparticle biosensor for maltose, which could be applied to other proteins with ligand-dependent conformation changes.