PubMed:16131178
Annnotations
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 179-186 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T2 | 564-571 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T3 | 604-611 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T4 | 731-738 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T5 | 1093-1100 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T6 | 1140-1147 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T7 | 1259-1266 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T8 | 1300-1307 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T9 | 1370-1377 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T10 | 1499-1506 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T11 | 1780-1787 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-78 | Sentence | denotes | A modular nanoparticle-based system for reagentless small molecule biosensing. |
| T2 | 79-198 | Sentence | denotes | Metalloprotein tethered CdSe nanoparticles have been generated to provide selective and reagentless maltose biosensing. |
| T3 | 199-398 | Sentence | denotes | As opposed to cell or protein detection by semiconducting nanoparticle bioconjugates, a modular method for small-molecule detection using semiconducting nanoparticle bioconjugates has been difficult. |
| T4 | 399-492 | Sentence | denotes | Here we report a method for reagentless protein-based semiconducting nanoparticle biosensors. |
| T5 | 493-691 | Sentence | denotes | This method uses Ru(II) complex-CdSe nanoparticle interactions and the maltose-induced conformation changes of maltose binding protein to alter the CdSe nanoparticle fluorescence emission intensity. |
| T6 | 692-876 | Sentence | denotes | In this proof-of-principle system, the maltose-induced protein conformation changes alter the Ru(II) complex-CdSe nanoparticle interaction, which increases the CdSe emission intensity. |
| T7 | 877-1045 | Sentence | denotes | Altered CdSe emission intensity effects are best described as electron transfer from the Ru(II) complex to the CdSe excited state forming the nonfluorescent CdSe anion. |
| T8 | 1046-1198 | Sentence | denotes | Four surface-cysteine, Ru(II) complex-attached maltose-binding proteins have been studied for maltose dependent alteration of CdSe emission intensities. |
| T9 | 1199-1416 | Sentence | denotes | With 3.0-3.5 nm diameter CdSe nanoparticles, all ruthenated maltose-binding proteins display similar maltose-dependent increases (1.4-fold) in CdSe emission intensity and maltose binding affinities (KA = 3 x 106 M-1). |
| T10 | 1417-1527 | Sentence | denotes | For these four systems, the only difference was the sample-to-sample variation in maltose-dependent responses. |
| T11 | 1528-1681 | Sentence | denotes | Thus, very few surface cysteine mutations need to be examined to find a successful biosensor, as opposed to analogous systems using organic fluorophores. |
| T12 | 1682-1873 | Sentence | denotes | This strategy generates a unimolecular, or reagentless, semiconducting nanoparticle biosensor for maltose, which could be applied to other proteins with ligand-dependent conformation changes. |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 179-186 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T2 | 564-571 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T3 | 604-611 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T4 | 731-738 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T5 | 1093-1100 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T6 | 1140-1147 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T7 | 1259-1266 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T8 | 1300-1307 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T9 | 1370-1377 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T10 | 1499-1506 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |
| T11 | 1780-1787 | Glycan | denotes | maltose | https://api.glycosmos.org/wurcs2image/latest/png/binary/G44653LT |