PubMed:16101297 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/16101297","sourcedb":"PubMed","sourceid":"16101297","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/16101297","text":"A novel conotoxin from Conus delessertii with posttranslationally modified lysine residues.\nA major peptide, de13a from the crude venom of Conus delessertii collected in the Yucatan Channel, Mexico, was purified. The peptide had a high content of posttranslationally modified amino acids, including 6-bromotryptophan and a nonstandard amino acid that proved to be 5-hydroxylysine. This is the first report of 5-hydroxylysine residues in conotoxins. The sequence analysis, together with cDNA cloning and a mass determination (monoisotopic mass of 3486.76 Da), established that the mature toxin has the sequence DCOTSCOTTCANGWECCKGYOCVNKACSGCTH, where O is 4-hydroxyproline, W 6-bromotryptophan, and K 5-hydroxylysine, the asterisk represents the amidated C-terminus, and the calculated monoisotopic mass is 3487.09 Da. The eight Cys residues are arranged in a pattern (C-C-C-CC-C-C-C) not described previously in conotoxins. This arrangement, for which we propose the designation of framework #13 or XIII, differs from the ones (C-C-CC-CC-C-C and C-C-C-C-CC-C-C) present in other conotoxins which also contain eight Cys residues. This peptide thus defines a novel class of conotoxins, with a new posttranslational modification not previously found in other Conus peptide families.","tracks":[{"project":"AnEM_abstracts","denotations":[{"id":"T1","span":{"begin":130,"end":135},"obj":"Organism_substance"}],"attributes":[{"subj":"T1","pred":"source","obj":"AnEM_abstracts"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"AnEM_abstracts","color":"#9f93ec","default":true}]}]}}