PubMed:16005859 JSONTXT

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":66,"end":69},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":71,"end":74},"obj":"Glycan_Motif"},{"id":"T5","span":{"begin":76,"end":79},"obj":"Glycan_Motif"},{"id":"T7","span":{"begin":81,"end":84},"obj":"Glycan_Motif"},{"id":"T9","span":{"begin":86,"end":89},"obj":"Glycan_Motif"},{"id":"T11","span":{"begin":91,"end":94},"obj":"Glycan_Motif"},{"id":"T13","span":{"begin":100,"end":104},"obj":"Glycan_Motif"},{"id":"T15","span":{"begin":576,"end":579},"obj":"Glycan_Motif"},{"id":"T17","span":{"begin":664,"end":667},"obj":"Glycan_Motif"},{"id":"T19","span":{"begin":776,"end":779},"obj":"Glycan_Motif"},{"id":"T21","span":{"begin":865,"end":868},"obj":"Glycan_Motif"},{"id":"T23","span":{"begin":978,"end":981},"obj":"Glycan_Motif"},{"id":"T25","span":{"begin":1119,"end":1122},"obj":"Glycan_Motif"},{"id":"T27","span":{"begin":1363,"end":1366},"obj":"Glycan_Motif"},{"id":"T29","span":{"begin":1380,"end":1384},"obj":"Glycan_Motif"},{"id":"T31","span":{"begin":1569,"end":1588},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH"},{"id":"A2","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G93899SO"},{"id":"A4","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G33570YF"},{"id":"A5","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G79389NT"},{"id":"A6","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G61168WC"},{"id":"A7","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G41759HA"},{"id":"A8","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G02657AK"},{"id":"A9","pred":"image","subj":"T9","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G36476BA"},{"id":"A10","pred":"image","subj":"T9","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G35228ZS"},{"id":"A11","pred":"image","subj":"T11","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G48558GR"},{"id":"A12","pred":"image","subj":"T11","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46613JI"},{"id":"A13","pred":"image","subj":"T13","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G69277LC"},{"id":"A14","pred":"image","subj":"T13","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46677TE"},{"id":"A15","pred":"image","subj":"T15","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G98544DH"},{"id":"A16","pred":"image","subj":"T15","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81928IQ"},{"id":"A17","pred":"image","subj":"T17","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G93899SO"},{"id":"A18","pred":"image","subj":"T17","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G33570YF"},{"id":"A19","pred":"image","subj":"T19","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G79389NT"},{"id":"A20","pred":"image","subj":"T19","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G61168WC"},{"id":"A21","pred":"image","subj":"T21","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G41759HA"},{"id":"A22","pred":"image","subj":"T21","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G02657AK"},{"id":"A23","pred":"image","subj":"T23","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G36476BA"},{"id":"A24","pred":"image","subj":"T23","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G35228ZS"},{"id":"A25","pred":"image","subj":"T25","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G48558GR"},{"id":"A26","pred":"image","subj":"T25","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46613JI"},{"id":"A27","pred":"image","subj":"T27","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G48558GR"},{"id":"A28","pred":"image","subj":"T27","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46613JI"},{"id":"A29","pred":"image","subj":"T29","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G69277LC"},{"id":"A30","pred":"image","subj":"T29","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46677TE"},{"id":"A31","pred":"image","subj":"T31","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G64581RP"}],"text":"Chemoenzymatic synthesis of 2-azidoethyl-ganglio-oligosaccharides GD3, GT3, GM2, GD2, GT2, GM1, and GD1a.\nWe have synthesized several ganglio-oligosaccharide structures using glycosyltransferases from Campylobacter jejuni. The enzymes, alpha-(2--\u003e3/8)-sialyltransferase (Cst-II), beta-(1--\u003e4)-N-acetylgalactosaminyltransferase (CgtA), and beta-(1--\u003e3)-galactosyltransferase (CgtB), were produced in large-scale fermentation from Escherichia coli and further characterized based on their acceptor specificities. 2-Azidoethyl-glycosides corresponding to the oligosaccharides of GD3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GM2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GD2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), and GM1 (beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) were synthesized in high yields (gram-scale). In addition, a mammalian alpha-(2--\u003e3)-sialyltransferase (ST3Gal I) was used to sialylate GM1 and generate GD1a (alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) oligosaccharide. We also cloned and expressed a rat UDP-N-acetylglucosamine-4'epimerase (GalNAcE) in E. coli AD202 cells for cost saving in situ conversion of less expensive UDP-GlcNAc to UDP-GalNAc."}

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":66,"end":69},"obj":"https://glytoucan.org/Structures/Glycans/G81928IQ"},{"id":"T2","span":{"begin":66,"end":69},"obj":"https://glytoucan.org/Structures/Glycans/G98544DH"},{"id":"T3","span":{"begin":71,"end":74},"obj":"https://glytoucan.org/Structures/Glycans/G33570YF"},{"id":"T4","span":{"begin":71,"end":74},"obj":"https://glytoucan.org/Structures/Glycans/G93899SO"},{"id":"T5","span":{"begin":76,"end":79},"obj":"https://glytoucan.org/Structures/Glycans/G61168WC"},{"id":"T6","span":{"begin":76,"end":79},"obj":"https://glytoucan.org/Structures/Glycans/G79389NT"},{"id":"T7","span":{"begin":81,"end":84},"obj":"https://glytoucan.org/Structures/Glycans/G02657AK"},{"id":"T8","span":{"begin":81,"end":84},"obj":"https://glytoucan.org/Structures/Glycans/G41759HA"},{"id":"T9","span":{"begin":86,"end":89},"obj":"https://glytoucan.org/Structures/Glycans/G35228ZS"},{"id":"T10","span":{"begin":86,"end":89},"obj":"https://glytoucan.org/Structures/Glycans/G36476BA"},{"id":"T11","span":{"begin":91,"end":94},"obj":"https://glytoucan.org/Structures/Glycans/G46613JI"},{"id":"T12","span":{"begin":91,"end":94},"obj":"https://glytoucan.org/Structures/Glycans/G48558GR"},{"id":"T13","span":{"begin":100,"end":104},"obj":"https://glytoucan.org/Structures/Glycans/G46677TE"},{"id":"T14","span":{"begin":100,"end":104},"obj":"https://glytoucan.org/Structures/Glycans/G69277LC"},{"id":"T15","span":{"begin":576,"end":579},"obj":"https://glytoucan.org/Structures/Glycans/G81928IQ"},{"id":"T16","span":{"begin":576,"end":579},"obj":"https://glytoucan.org/Structures/Glycans/G98544DH"},{"id":"T17","span":{"begin":664,"end":667},"obj":"https://glytoucan.org/Structures/Glycans/G33570YF"},{"id":"T18","span":{"begin":664,"end":667},"obj":"https://glytoucan.org/Structures/Glycans/G93899SO"},{"id":"T19","span":{"begin":776,"end":779},"obj":"https://glytoucan.org/Structures/Glycans/G61168WC"},{"id":"T20","span":{"begin":776,"end":779},"obj":"https://glytoucan.org/Structures/Glycans/G79389NT"},{"id":"T21","span":{"begin":865,"end":868},"obj":"https://glytoucan.org/Structures/Glycans/G02657AK"},{"id":"T22","span":{"begin":865,"end":868},"obj":"https://glytoucan.org/Structures/Glycans/G41759HA"},{"id":"T23","span":{"begin":978,"end":981},"obj":"https://glytoucan.org/Structures/Glycans/G35228ZS"},{"id":"T24","span":{"begin":978,"end":981},"obj":"https://glytoucan.org/Structures/Glycans/G36476BA"},{"id":"T25","span":{"begin":1119,"end":1122},"obj":"https://glytoucan.org/Structures/Glycans/G46613JI"},{"id":"T26","span":{"begin":1119,"end":1122},"obj":"https://glytoucan.org/Structures/Glycans/G48558GR"},{"id":"T27","span":{"begin":1363,"end":1366},"obj":"https://glytoucan.org/Structures/Glycans/G46613JI"},{"id":"T28","span":{"begin":1363,"end":1366},"obj":"https://glytoucan.org/Structures/Glycans/G48558GR"},{"id":"T29","span":{"begin":1380,"end":1384},"obj":"https://glytoucan.org/Structures/Glycans/G46677TE"},{"id":"T30","span":{"begin":1380,"end":1384},"obj":"https://glytoucan.org/Structures/Glycans/G69277LC"},{"id":"T31","span":{"begin":1569,"end":1588},"obj":"https://glytoucan.org/Structures/Glycans/G64581RP"}],"text":"Chemoenzymatic synthesis of 2-azidoethyl-ganglio-oligosaccharides GD3, GT3, GM2, GD2, GT2, GM1, and GD1a.\nWe have synthesized several ganglio-oligosaccharide structures using glycosyltransferases from Campylobacter jejuni. The enzymes, alpha-(2--\u003e3/8)-sialyltransferase (Cst-II), beta-(1--\u003e4)-N-acetylgalactosaminyltransferase (CgtA), and beta-(1--\u003e3)-galactosyltransferase (CgtB), were produced in large-scale fermentation from Escherichia coli and further characterized based on their acceptor specificities. 2-Azidoethyl-glycosides corresponding to the oligosaccharides of GD3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GM2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GD2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), and GM1 (beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) were synthesized in high yields (gram-scale). In addition, a mammalian alpha-(2--\u003e3)-sialyltransferase (ST3Gal I) was used to sialylate GM1 and generate GD1a (alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) oligosaccharide. We also cloned and expressed a rat UDP-N-acetylglucosamine-4'epimerase (GalNAcE) in E. coli AD202 cells for cost saving in situ conversion of less expensive UDP-GlcNAc to UDP-GalNAc."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":105},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":106,"end":222},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":223,"end":510},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":511,"end":1272},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":1273,"end":1529},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":1530,"end":1712},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":105},"obj":"Sentence"},{"id":"T2","span":{"begin":106,"end":222},"obj":"Sentence"},{"id":"T3","span":{"begin":223,"end":510},"obj":"Sentence"},{"id":"T4","span":{"begin":511,"end":1272},"obj":"Sentence"},{"id":"T5","span":{"begin":1273,"end":1529},"obj":"Sentence"},{"id":"T6","span":{"begin":1530,"end":1712},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Chemoenzymatic synthesis of 2-azidoethyl-ganglio-oligosaccharides GD3, GT3, GM2, GD2, GT2, GM1, and GD1a.\nWe have synthesized several ganglio-oligosaccharide structures using glycosyltransferases from Campylobacter jejuni. The enzymes, alpha-(2--\u003e3/8)-sialyltransferase (Cst-II), beta-(1--\u003e4)-N-acetylgalactosaminyltransferase (CgtA), and beta-(1--\u003e3)-galactosyltransferase (CgtB), were produced in large-scale fermentation from Escherichia coli and further characterized based on their acceptor specificities. 2-Azidoethyl-glycosides corresponding to the oligosaccharides of GD3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GM2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GD2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), and GM1 (beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) were synthesized in high yields (gram-scale). In addition, a mammalian alpha-(2--\u003e3)-sialyltransferase (ST3Gal I) was used to sialylate GM1 and generate GD1a (alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) oligosaccharide. We also cloned and expressed a rat UDP-N-acetylglucosamine-4'epimerase (GalNAcE) in E. coli AD202 cells for cost saving in situ conversion of less expensive UDP-GlcNAc to UDP-GalNAc."}

{"project":"Glycosmos6-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":71,"end":74},"obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"T2","span":{"begin":91,"end":94},"obj":"http://www.glycoepitope.jp/epitopes/EP0050"},{"id":"T3","span":{"begin":100,"end":104},"obj":"http://www.glycoepitope.jp/epitopes/EP0056"},{"id":"T4","span":{"begin":664,"end":667},"obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"T5","span":{"begin":1119,"end":1122},"obj":"http://www.glycoepitope.jp/epitopes/EP0050"},{"id":"T6","span":{"begin":1363,"end":1366},"obj":"http://www.glycoepitope.jp/epitopes/EP0050"},{"id":"T7","span":{"begin":1380,"end":1384},"obj":"http://www.glycoepitope.jp/epitopes/EP0056"}],"text":"Chemoenzymatic synthesis of 2-azidoethyl-ganglio-oligosaccharides GD3, GT3, GM2, GD2, GT2, GM1, and GD1a.\nWe have synthesized several ganglio-oligosaccharide structures using glycosyltransferases from Campylobacter jejuni. The enzymes, alpha-(2--\u003e3/8)-sialyltransferase (Cst-II), beta-(1--\u003e4)-N-acetylgalactosaminyltransferase (CgtA), and beta-(1--\u003e3)-galactosyltransferase (CgtB), were produced in large-scale fermentation from Escherichia coli and further characterized based on their acceptor specificities. 2-Azidoethyl-glycosides corresponding to the oligosaccharides of GD3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GM2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GD2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), and GM1 (beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) were synthesized in high yields (gram-scale). In addition, a mammalian alpha-(2--\u003e3)-sialyltransferase (ST3Gal I) was used to sialylate GM1 and generate GD1a (alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) oligosaccharide. We also cloned and expressed a rat UDP-N-acetylglucosamine-4'epimerase (GalNAcE) in E. coli AD202 cells for cost saving in situ conversion of less expensive UDP-GlcNAc to UDP-GalNAc."}

{"project":"NGLY1-deficiency","denotations":[{"id":"PD-NGLY1-deficiency-B_T1","span":{"begin":1569,"end":1588},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T2","span":{"begin":1691,"end":1697},"obj":"chem:24139"}],"namespaces":[{"prefix":"hgnc","uri":"https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:"},{"prefix":"omim","uri":"https://www.omim.org/entry/"},{"prefix":"chem","uri":"https://pubchem.ncbi.nlm.nih.gov/compound/"}],"text":"Chemoenzymatic synthesis of 2-azidoethyl-ganglio-oligosaccharides GD3, GT3, GM2, GD2, GT2, GM1, and GD1a.\nWe have synthesized several ganglio-oligosaccharide structures using glycosyltransferases from Campylobacter jejuni. The enzymes, alpha-(2--\u003e3/8)-sialyltransferase (Cst-II), beta-(1--\u003e4)-N-acetylgalactosaminyltransferase (CgtA), and beta-(1--\u003e3)-galactosyltransferase (CgtB), were produced in large-scale fermentation from Escherichia coli and further characterized based on their acceptor specificities. 2-Azidoethyl-glycosides corresponding to the oligosaccharides of GD3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GM2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GD2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), and GM1 (beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) were synthesized in high yields (gram-scale). In addition, a mammalian alpha-(2--\u003e3)-sialyltransferase (ST3Gal I) was used to sialylate GM1 and generate GD1a (alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) oligosaccharide. We also cloned and expressed a rat UDP-N-acetylglucosamine-4'epimerase (GalNAcE) in E. coli AD202 cells for cost saving in situ conversion of less expensive UDP-GlcNAc to UDP-GalNAc."}

{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":66,"end":69},"obj":"Glycan"},{"id":"T2","span":{"begin":71,"end":74},"obj":"Glycan"},{"id":"T3","span":{"begin":76,"end":79},"obj":"Glycan"},{"id":"T4","span":{"begin":81,"end":84},"obj":"Glycan"},{"id":"T5","span":{"begin":86,"end":89},"obj":"Glycan"},{"id":"T6","span":{"begin":91,"end":94},"obj":"Glycan"},{"id":"T7","span":{"begin":100,"end":104},"obj":"Glycan"},{"id":"T8","span":{"begin":576,"end":579},"obj":"Glycan"},{"id":"T9","span":{"begin":664,"end":667},"obj":"Glycan"},{"id":"T10","span":{"begin":776,"end":779},"obj":"Glycan"},{"id":"T11","span":{"begin":865,"end":868},"obj":"Glycan"},{"id":"T12","span":{"begin":978,"end":981},"obj":"Glycan"},{"id":"T13","span":{"begin":1119,"end":1122},"obj":"Glycan"},{"id":"T14","span":{"begin":1363,"end":1366},"obj":"Glycan"},{"id":"T15","span":{"begin":1380,"end":1384},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G98544DH"},{"id":"A16","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G93899SO"},{"id":"A17","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G93899SO"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G79389NT"},{"id":"A18","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT"},{"id":"A4","pred":"glycosmos_id","subj":"T4","obj":"https://glycosmos.org/glycans/show/G02657AK"},{"id":"A19","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK"},{"id":"A5","pred":"glycosmos_id","subj":"T5","obj":"https://glycosmos.org/glycans/show/G36476BA"},{"id":"A20","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G36476BA"},{"id":"A6","pred":"glycosmos_id","subj":"T6","obj":"https://glycosmos.org/glycans/show/G48558GR"},{"id":"A21","pred":"image","subj":"T6","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR"},{"id":"A7","pred":"glycosmos_id","subj":"T7","obj":"https://glycosmos.org/glycans/show/G46677TE"},{"id":"A22","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE"},{"id":"A8","pred":"glycosmos_id","subj":"T8","obj":"https://glycosmos.org/glycans/show/G98544DH"},{"id":"A23","pred":"image","subj":"T8","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G98544DH"},{"id":"A9","pred":"glycosmos_id","subj":"T9","obj":"https://glycosmos.org/glycans/show/G93899SO"},{"id":"A24","pred":"image","subj":"T9","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G93899SO"},{"id":"A10","pred":"glycosmos_id","subj":"T10","obj":"https://glycosmos.org/glycans/show/G79389NT"},{"id":"A25","pred":"image","subj":"T10","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT"},{"id":"A11","pred":"glycosmos_id","subj":"T11","obj":"https://glycosmos.org/glycans/show/G02657AK"},{"id":"A26","pred":"image","subj":"T11","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G02657AK"},{"id":"A12","pred":"glycosmos_id","subj":"T12","obj":"https://glycosmos.org/glycans/show/G36476BA"},{"id":"A27","pred":"image","subj":"T12","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G36476BA"},{"id":"A13","pred":"glycosmos_id","subj":"T13","obj":"https://glycosmos.org/glycans/show/G48558GR"},{"id":"A28","pred":"image","subj":"T13","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR"},{"id":"A14","pred":"glycosmos_id","subj":"T14","obj":"https://glycosmos.org/glycans/show/G48558GR"},{"id":"A29","pred":"image","subj":"T14","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR"},{"id":"A15","pred":"glycosmos_id","subj":"T15","obj":"https://glycosmos.org/glycans/show/G46677TE"},{"id":"A30","pred":"image","subj":"T15","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE"}],"text":"Chemoenzymatic synthesis of 2-azidoethyl-ganglio-oligosaccharides GD3, GT3, GM2, GD2, GT2, GM1, and GD1a.\nWe have synthesized several ganglio-oligosaccharide structures using glycosyltransferases from Campylobacter jejuni. The enzymes, alpha-(2--\u003e3/8)-sialyltransferase (Cst-II), beta-(1--\u003e4)-N-acetylgalactosaminyltransferase (CgtA), and beta-(1--\u003e3)-galactosyltransferase (CgtB), were produced in large-scale fermentation from Escherichia coli and further characterized based on their acceptor specificities. 2-Azidoethyl-glycosides corresponding to the oligosaccharides of GD3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GM2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GD2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), and GM1 (beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) were synthesized in high yields (gram-scale). In addition, a mammalian alpha-(2--\u003e3)-sialyltransferase (ST3Gal I) was used to sialylate GM1 and generate GD1a (alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) oligosaccharide. We also cloned and expressed a rat UDP-N-acetylglucosamine-4'epimerase (GalNAcE) in E. coli AD202 cells for cost saving in situ conversion of less expensive UDP-GlcNAc to UDP-GalNAc."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":201,"end":221},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":429,"end":445},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":1561,"end":1564},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":1614,"end":1621},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"197"},{"id":"A2","pred":"db_id","subj":"T2","obj":"562"},{"id":"A3","pred":"db_id","subj":"T3","obj":"10114"},{"id":"A4","pred":"db_id","subj":"T3","obj":"10116"},{"id":"A5","pred":"db_id","subj":"T5","obj":"562"}],"text":"Chemoenzymatic synthesis of 2-azidoethyl-ganglio-oligosaccharides GD3, GT3, GM2, GD2, GT2, GM1, and GD1a.\nWe have synthesized several ganglio-oligosaccharide structures using glycosyltransferases from Campylobacter jejuni. The enzymes, alpha-(2--\u003e3/8)-sialyltransferase (Cst-II), beta-(1--\u003e4)-N-acetylgalactosaminyltransferase (CgtA), and beta-(1--\u003e3)-galactosyltransferase (CgtB), were produced in large-scale fermentation from Escherichia coli and further characterized based on their acceptor specificities. 2-Azidoethyl-glycosides corresponding to the oligosaccharides of GD3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GM2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GD2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), and GM1 (beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) were synthesized in high yields (gram-scale). In addition, a mammalian alpha-(2--\u003e3)-sialyltransferase (ST3Gal I) was used to sialylate GM1 and generate GD1a (alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) oligosaccharide. We also cloned and expressed a rat UDP-N-acetylglucosamine-4'epimerase (GalNAcE) in E. coli AD202 cells for cost saving in situ conversion of less expensive UDP-GlcNAc to UDP-GalNAc."}

{"project":"Glycosmos15-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":71,"end":74},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":91,"end":94},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T3","span":{"begin":100,"end":104},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T4","span":{"begin":664,"end":667},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T5","span":{"begin":1119,"end":1122},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T6","span":{"begin":1363,"end":1366},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T7","span":{"begin":1380,"end":1384},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0050"},{"id":"A3","pred":"glycoepitope_id","subj":"T3","obj":"http://www.glycoepitope.jp/epitopes/EP0056"},{"id":"A4","pred":"glycoepitope_id","subj":"T4","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A5","pred":"glycoepitope_id","subj":"T5","obj":"http://www.glycoepitope.jp/epitopes/EP0050"},{"id":"A6","pred":"glycoepitope_id","subj":"T6","obj":"http://www.glycoepitope.jp/epitopes/EP0050"},{"id":"A7","pred":"glycoepitope_id","subj":"T7","obj":"http://www.glycoepitope.jp/epitopes/EP0056"}],"text":"Chemoenzymatic synthesis of 2-azidoethyl-ganglio-oligosaccharides GD3, GT3, GM2, GD2, GT2, GM1, and GD1a.\nWe have synthesized several ganglio-oligosaccharide structures using glycosyltransferases from Campylobacter jejuni. The enzymes, alpha-(2--\u003e3/8)-sialyltransferase (Cst-II), beta-(1--\u003e4)-N-acetylgalactosaminyltransferase (CgtA), and beta-(1--\u003e3)-galactosyltransferase (CgtB), were produced in large-scale fermentation from Escherichia coli and further characterized based on their acceptor specificities. 2-Azidoethyl-glycosides corresponding to the oligosaccharides of GD3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GM2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GD2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), and GM1 (beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) were synthesized in high yields (gram-scale). In addition, a mammalian alpha-(2--\u003e3)-sialyltransferase (ST3Gal I) was used to sialylate GM1 and generate GD1a (alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) oligosaccharide. We also cloned and expressed a rat UDP-N-acetylglucosamine-4'epimerase (GalNAcE) in E. coli AD202 cells for cost saving in situ conversion of less expensive UDP-GlcNAc to UDP-GalNAc."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":405,"end":410},"obj":"Body_part"},{"id":"T2","span":{"begin":1265,"end":1270},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0002542"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0002542"}],"text":"Chemoenzymatic synthesis of 2-azidoethyl-ganglio-oligosaccharides GD3, GT3, GM2, GD2, GT2, GM1, and GD1a.\nWe have synthesized several ganglio-oligosaccharide structures using glycosyltransferases from Campylobacter jejuni. The enzymes, alpha-(2--\u003e3/8)-sialyltransferase (Cst-II), beta-(1--\u003e4)-N-acetylgalactosaminyltransferase (CgtA), and beta-(1--\u003e3)-galactosyltransferase (CgtB), were produced in large-scale fermentation from Escherichia coli and further characterized based on their acceptor specificities. 2-Azidoethyl-glycosides corresponding to the oligosaccharides of GD3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT3 (alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GM2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GD2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), GT2 (beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e8)-alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-), and GM1 (beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) were synthesized in high yields (gram-scale). In addition, a mammalian alpha-(2--\u003e3)-sialyltransferase (ST3Gal I) was used to sialylate GM1 and generate GD1a (alpha-D-Neup5Ac-(2--\u003e3)-beta-D-Galp-(1--\u003e3)-beta-D-GalpNAc-(1--\u003e4)-[alpha-D-Neup5Ac-(2--\u003e3)]-beta-D-Galp-(1--\u003e4)-beta-D-Glcp-) oligosaccharide. We also cloned and expressed a rat UDP-N-acetylglucosamine-4'epimerase (GalNAcE) in E. coli AD202 cells for cost saving in situ conversion of less expensive UDP-GlcNAc to UDP-GalNAc."}