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PubMed:16005455 JSONTXT

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PubMed_ArguminSci

Id Subject Object Predicate Lexical cue
T1 116-208 DRI_Approach denotes Protein kinase C (PKC) activation and increases in cytosolic Ca(2+) cause intestinal injury.
T2 209-412 DRI_Outcome denotes Since PKC activation can alter Ca(2+) homeostasis and increase Ca(2+) levels, we examined the effects of PKC activation on intestinal cellular integrity and the role of Ca(2+) signaling in this response.
T3 413-514 DRI_Background denotes The epithelial cell line, IEC-18 was incubated with the PKC activator phorbol myristate acetate (PMA;
T4 529-531 DRI_Background denotes ).
T5 532-596 DRI_Background denotes In some experiments, cells were incubated in Ca(2+)-free medium.
T6 597-687 DRI_Background denotes PMA treatment produced a concentration-dependent increase in cell injury and PKC activity.
T7 688-775 DRI_Approach denotes This response was attenuated by addition of the pan-specific PKC inhibitor, GF 109203X.
T8 776-915 DRI_Background denotes Furthermore, cell viability was maintained in cells preincubated with PKC isoform-specific inhibitors to PKCalpha, PKCdelta and PKCepsilon.
T9 916-1074 DRI_Approach denotes Cell injury was also reduced if cells were incubated in Ca(2+)-free medium or in the presence of the Ca(2+) channel antagonist, verapamil or the intracellular
T10 1092-1093 DRI_Approach denotes .
T11 1094-1318 DRI_Approach denotes PMA, but not the inactive phorbol ester, 4alphaPMA, induced a dose-dependent increase in cellular Ca(2+) that was characterized by a rapid, transient spike followed by a tonic plateau phase which approximated control levels.
T12 1319-1379 DRI_Approach denotes These responses were eliminated by the addition of BAPTA-AM.
T13 1380-1508 DRI_Outcome denotes Furthermore the increase in the Ca(2+) spike was reduced or eliminated by co-incubation with the PKCdelta antagonist, rottlerin.
T14 1509-1595 DRI_Background denotes Inhibition of PKCalpha or PKCepsilon was less effective or ineffective in this regard.
T15 1596-1710 DRI_Outcome denotes These data suggest that PKC activation via PMA challenge affects the integrity of rat intestinal epithelial cells.
T16 1711-1829 DRI_Background denotes PKCdelta, but not PKCepsilon or PKCalpha activation appears to mediate this effect via an increase in cellular Ca(2+).