PubMed:1591763 JSONTXT

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":562,"end":571},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G68158BT"},{"id":"A2","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G65889KE"}],"text":"New investigations of the structure of grape arabinogalactan-protein.\nThe structure of an arabinogalactan-protein (AGP) isolated from grape juice was studied by methylation analysis, n.m.r. spectroscopy, and interactions with peanut lectin, after specific degradation with purified enzymes and/or Smith degradation. AGP appeared to be homogeneous with a weight-average molecular weight of 110,000. Treatment of AGP with arabinofuranosidase released 88% of the arabinose and left GP1. Hydrolysis of GP1 with an endo-(1----6)-beta-D-galactanase removed 50% of the galactose and left GP2. Smith degradation of GP1 gave a 3-linked galactan that still contained 3,6-linked residues. Endogalactanase- and Smith-degraded GP1, but not AGP and GP1, reacted strongly with peanut lectin. Thus, AGP is a 3-linked galactan cross-linked at positions 6. The core also carries, at positions 6, 6-linked galactan chains heavily 3-substituted with arabinofuranose residues."}

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":562,"end":571},"obj":"https://glytoucan.org/Structures/Glycans/G65889KE"},{"id":"T2","span":{"begin":562,"end":571},"obj":"https://glytoucan.org/Structures/Glycans/G68158BT"}],"text":"New investigations of the structure of grape arabinogalactan-protein.\nThe structure of an arabinogalactan-protein (AGP) isolated from grape juice was studied by methylation analysis, n.m.r. spectroscopy, and interactions with peanut lectin, after specific degradation with purified enzymes and/or Smith degradation. AGP appeared to be homogeneous with a weight-average molecular weight of 110,000. Treatment of AGP with arabinofuranosidase released 88% of the arabinose and left GP1. Hydrolysis of GP1 with an endo-(1----6)-beta-D-galactanase removed 50% of the galactose and left GP2. Smith degradation of GP1 gave a 3-linked galactan that still contained 3,6-linked residues. Endogalactanase- and Smith-degraded GP1, but not AGP and GP1, reacted strongly with peanut lectin. Thus, AGP is a 3-linked galactan cross-linked at positions 6. The core also carries, at positions 6, 6-linked galactan chains heavily 3-substituted with arabinofuranose residues."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":69},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":70,"end":315},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":316,"end":397},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":398,"end":483},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":484,"end":585},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":586,"end":677},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":678,"end":776},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":777,"end":838},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":839,"end":955},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":69},"obj":"Sentence"},{"id":"T2","span":{"begin":70,"end":315},"obj":"Sentence"},{"id":"T3","span":{"begin":316,"end":397},"obj":"Sentence"},{"id":"T4","span":{"begin":398,"end":483},"obj":"Sentence"},{"id":"T5","span":{"begin":484,"end":585},"obj":"Sentence"},{"id":"T6","span":{"begin":586,"end":677},"obj":"Sentence"},{"id":"T7","span":{"begin":678,"end":776},"obj":"Sentence"},{"id":"T8","span":{"begin":777,"end":838},"obj":"Sentence"},{"id":"T9","span":{"begin":839,"end":955},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"New investigations of the structure of grape arabinogalactan-protein.\nThe structure of an arabinogalactan-protein (AGP) isolated from grape juice was studied by methylation analysis, n.m.r. spectroscopy, and interactions with peanut lectin, after specific degradation with purified enzymes and/or Smith degradation. AGP appeared to be homogeneous with a weight-average molecular weight of 110,000. Treatment of AGP with arabinofuranosidase released 88% of the arabinose and left GP1. Hydrolysis of GP1 with an endo-(1----6)-beta-D-galactanase removed 50% of the galactose and left GP2. Smith degradation of GP1 gave a 3-linked galactan that still contained 3,6-linked residues. Endogalactanase- and Smith-degraded GP1, but not AGP and GP1, reacted strongly with peanut lectin. Thus, AGP is a 3-linked galactan cross-linked at positions 6. The core also carries, at positions 6, 6-linked galactan chains heavily 3-substituted with arabinofuranose residues."}