PubMed:15914610 / 245-772 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/15914610","sourcedb":"PubMed","sourceid":"15914610","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/15914610","text":"A gene-targeting construct containing an internal ribosomal entry site-reverse tetracycline transcription activator (IRES-rtTA) cassette was inserted into the Krt12 allele (keratin 12 gene) to produce a knock-in Krt12(rtTA/+) mouse line through gene-targeting techniques. The Krt12(rtTA/+) knock-in mice were bred with tet-O-LacZ reporter mice to obtain Krt12(rtTA/+)/tet-O-LacZ bitransgenic mice. The expression of the LacZ gene was induced in bitransgenic mice by administration of doxycycline in the drinking water and chow.","tracks":[{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T2","span":{"begin":0,"end":527},"obj":"METHODS"}],"attributes":[{"subj":"T2","pred":"source","obj":"PubMed_Structured_Abstracts"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubMed_Structured_Abstracts","color":"#ecd093","default":true}]}]}}