PubMed:15866877
Annnotations
GlyCosmos6-Glycan-Motif-Image
Id | Subject | Object | Predicate | Lexical cue | image |
---|---|---|---|---|---|
T1 | 898-906 | Glycan_Motif | denotes | arabinan | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G22535ZZ |
T2 | 931-939 | Glycan_Motif | denotes | arabinan | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G22535ZZ |
T3 | 1361-1370 | Glycan_Motif | denotes | galactose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G68158BT|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G65889KE |
T5 | 1595-1604 | Glycan_Motif | denotes | galactose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G68158BT|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G65889KE |
T7 | 1968-1977 | Glycan_Motif | denotes | galactose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G68158BT|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G65889KE |
T9 | 2180-2189 | Glycan_Motif | denotes | galactose | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G68158BT|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G65889KE |
GlyCosmos6-Glycan-Motif-Structure
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 898-906 | https://glytoucan.org/Structures/Glycans/G22535ZZ | denotes | arabinan |
T2 | 931-939 | https://glytoucan.org/Structures/Glycans/G22535ZZ | denotes | arabinan |
T3 | 1361-1370 | https://glytoucan.org/Structures/Glycans/G65889KE | denotes | galactose |
T4 | 1361-1370 | https://glytoucan.org/Structures/Glycans/G68158BT | denotes | galactose |
T5 | 1595-1604 | https://glytoucan.org/Structures/Glycans/G65889KE | denotes | galactose |
T6 | 1595-1604 | https://glytoucan.org/Structures/Glycans/G68158BT | denotes | galactose |
T7 | 1968-1977 | https://glytoucan.org/Structures/Glycans/G65889KE | denotes | galactose |
T8 | 1968-1977 | https://glytoucan.org/Structures/Glycans/G68158BT | denotes | galactose |
T9 | 2180-2189 | https://glytoucan.org/Structures/Glycans/G65889KE | denotes | galactose |
T10 | 2180-2189 | https://glytoucan.org/Structures/Glycans/G68158BT | denotes | galactose |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 0-109 | Sentence | denotes | An exo-beta-1,3-galactanase having a novel beta-1,3-galactan-binding module from Phanerochaete chrysosporium. |
T2 | 110-237 | Sentence | denotes | An exo-beta-1,3-galactanase gene from Phanerochaete chrysosporium has been cloned, sequenced, and expressed in Pichia pastoris. |
T3 | 238-545 | Sentence | denotes | The complete amino acid sequence of the exo-beta-1,3-galactanase indicated that the enzyme consists of an N-terminal catalytic module with similarity to glycoside hydrolase family 43 and an additional unknown functional domain similar to carbohydrate-binding module family 6 (CBM6) in the C-terminal region. |
T4 | 546-633 | Sentence | denotes | The molecular mass of the recombinant enzyme was estimated as 55 kDa based on SDS-PAGE. |
T5 | 634-1006 | Sentence | denotes | The enzyme showed reactivity only toward beta-1,3-linked galactosyl oligosaccharides and polysaccharide as substrates but did not hydrolyze beta-1,4-linked galacto-oligosaccharides, beta-1,6-linked galacto-oligosaccharides, pectic galactan, larch arabinogalactan, arabinan, gum arabic, debranched arabinan, laminarin, soluble birchwood xylan, or soluble oat spelled xylan. |
T6 | 1007-1252 | Sentence | denotes | The enzyme also did not hydrolyze beta-1,3-galactosyl galactosaminide, beta-1,3-galactosyl glucosaminide, or beta-1,3-galactosyl arabinofuranoside, suggesting that it specifically cleaves the internal beta-1,3-linkage of two galactosyl residues. |
T7 | 1253-1418 | Sentence | denotes | High performance liquid chromatographic analysis of the hydrolysis products showed that the enzyme produced galactose from beta-1,3-galactan in an exo-acting manner. |
T8 | 1419-1497 | Sentence | denotes | However, no activity toward p-nitrophenyl beta-galactopyranoside was detected. |
T9 | 1498-1682 | Sentence | denotes | When incubated with arabinogalactan proteins, the enzyme produced oligosaccharides together with galactose, suggesting that it is able to bypass beta-1,6-linked galactosyl side chains. |
T10 | 1683-1883 | Sentence | denotes | The C-terminal CBM6 did not show any affinity for known substrates of CBM6 such as xylan, cellulose, and beta-1,3-glucan, although it bound beta-1,3-galactan when analyzed by affinity electrophoresis. |
T11 | 1884-2142 | Sentence | denotes | Frontal affinity chromatography for the CBM6 moiety using several kinds of terminal galactose-containing oligosaccharides as the analytes clearly indicated that the CBM6 specifically interacted with oligosaccharides containing a beta-1,3-galactobiose moiety. |
T12 | 2143-2272 | Sentence | denotes | When the degree of polymerization of galactose oligomers was increased, the binding affinity of the CBM6 showed no marked change. |
Glycosmos6-GlycoEpitope
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 898-906 | http://www.glycoepitope.jp/epitopes/EP0508 | denotes | arabinan |
T2 | 931-939 | http://www.glycoepitope.jp/epitopes/EP0508 | denotes | arabinan |
Glycan-GlyCosmos
Id | Subject | Object | Predicate | Lexical cue | image |
---|---|---|---|---|---|
T1 | 898-906 | Glycan | denotes | arabinan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G22535ZZ |
T2 | 931-939 | Glycan | denotes | arabinan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G22535ZZ |
GlyCosmos-GlycoEpitope
Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
---|---|---|---|---|---|
T1 | 898-906 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | arabinan | http://www.glycoepitope.jp/epitopes/EP0508 |
T2 | 931-939 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | arabinan | http://www.glycoepitope.jp/epitopes/EP0508 |
GlyCosmos15-NCBITAXON
Id | Subject | Object | Predicate | Lexical cue | db_id |
---|---|---|---|---|---|
T1 | 81-108 | OrganismTaxon | denotes | Phanerochaete chrysosporium | 2822231 |
T2 | 148-175 | OrganismTaxon | denotes | Phanerochaete chrysosporium | 2822231 |
T3 | 221-236 | OrganismTaxon | denotes | Pichia pastoris | 4922 |
T4 | 908-918 | OrganismTaxon | denotes | gum arabic | 138043 |
GlyCosmos15-UBERON
Id | Subject | Object | Predicate | Lexical cue | uberon_id |
---|---|---|---|---|---|
T1 | 908-911 | Body_part | denotes | gum | http://purl.obolibrary.org/obo/UBERON_0001828 |
T2 | 1884-1891 | Body_part | denotes | Frontal | http://purl.obolibrary.org/obo/UBERON_0000209 |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 0-109 | Sentence | denotes | An exo-beta-1,3-galactanase having a novel beta-1,3-galactan-binding module from Phanerochaete chrysosporium. |
T2 | 110-237 | Sentence | denotes | An exo-beta-1,3-galactanase gene from Phanerochaete chrysosporium has been cloned, sequenced, and expressed in Pichia pastoris. |
T3 | 238-545 | Sentence | denotes | The complete amino acid sequence of the exo-beta-1,3-galactanase indicated that the enzyme consists of an N-terminal catalytic module with similarity to glycoside hydrolase family 43 and an additional unknown functional domain similar to carbohydrate-binding module family 6 (CBM6) in the C-terminal region. |
T4 | 546-633 | Sentence | denotes | The molecular mass of the recombinant enzyme was estimated as 55 kDa based on SDS-PAGE. |
T5 | 634-1006 | Sentence | denotes | The enzyme showed reactivity only toward beta-1,3-linked galactosyl oligosaccharides and polysaccharide as substrates but did not hydrolyze beta-1,4-linked galacto-oligosaccharides, beta-1,6-linked galacto-oligosaccharides, pectic galactan, larch arabinogalactan, arabinan, gum arabic, debranched arabinan, laminarin, soluble birchwood xylan, or soluble oat spelled xylan. |
T6 | 1007-1252 | Sentence | denotes | The enzyme also did not hydrolyze beta-1,3-galactosyl galactosaminide, beta-1,3-galactosyl glucosaminide, or beta-1,3-galactosyl arabinofuranoside, suggesting that it specifically cleaves the internal beta-1,3-linkage of two galactosyl residues. |
T7 | 1253-1418 | Sentence | denotes | High performance liquid chromatographic analysis of the hydrolysis products showed that the enzyme produced galactose from beta-1,3-galactan in an exo-acting manner. |
T8 | 1419-1497 | Sentence | denotes | However, no activity toward p-nitrophenyl beta-galactopyranoside was detected. |
T9 | 1498-1682 | Sentence | denotes | When incubated with arabinogalactan proteins, the enzyme produced oligosaccharides together with galactose, suggesting that it is able to bypass beta-1,6-linked galactosyl side chains. |
T10 | 1683-1883 | Sentence | denotes | The C-terminal CBM6 did not show any affinity for known substrates of CBM6 such as xylan, cellulose, and beta-1,3-glucan, although it bound beta-1,3-galactan when analyzed by affinity electrophoresis. |
T11 | 1884-2142 | Sentence | denotes | Frontal affinity chromatography for the CBM6 moiety using several kinds of terminal galactose-containing oligosaccharides as the analytes clearly indicated that the CBM6 specifically interacted with oligosaccharides containing a beta-1,3-galactobiose moiety. |
T12 | 2143-2272 | Sentence | denotes | When the degree of polymerization of galactose oligomers was increased, the binding affinity of the CBM6 showed no marked change. |
GlyCosmos15-Sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 0-109 | Sentence | denotes | An exo-beta-1,3-galactanase having a novel beta-1,3-galactan-binding module from Phanerochaete chrysosporium. |
T2 | 110-237 | Sentence | denotes | An exo-beta-1,3-galactanase gene from Phanerochaete chrysosporium has been cloned, sequenced, and expressed in Pichia pastoris. |
T3 | 238-545 | Sentence | denotes | The complete amino acid sequence of the exo-beta-1,3-galactanase indicated that the enzyme consists of an N-terminal catalytic module with similarity to glycoside hydrolase family 43 and an additional unknown functional domain similar to carbohydrate-binding module family 6 (CBM6) in the C-terminal region. |
T4 | 546-633 | Sentence | denotes | The molecular mass of the recombinant enzyme was estimated as 55 kDa based on SDS-PAGE. |
T5 | 634-1006 | Sentence | denotes | The enzyme showed reactivity only toward beta-1,3-linked galactosyl oligosaccharides and polysaccharide as substrates but did not hydrolyze beta-1,4-linked galacto-oligosaccharides, beta-1,6-linked galacto-oligosaccharides, pectic galactan, larch arabinogalactan, arabinan, gum arabic, debranched arabinan, laminarin, soluble birchwood xylan, or soluble oat spelled xylan. |
T6 | 1007-1252 | Sentence | denotes | The enzyme also did not hydrolyze beta-1,3-galactosyl galactosaminide, beta-1,3-galactosyl glucosaminide, or beta-1,3-galactosyl arabinofuranoside, suggesting that it specifically cleaves the internal beta-1,3-linkage of two galactosyl residues. |
T7 | 1253-1418 | Sentence | denotes | High performance liquid chromatographic analysis of the hydrolysis products showed that the enzyme produced galactose from beta-1,3-galactan in an exo-acting manner. |
T8 | 1419-1497 | Sentence | denotes | However, no activity toward p-nitrophenyl beta-galactopyranoside was detected. |
T9 | 1498-1682 | Sentence | denotes | When incubated with arabinogalactan proteins, the enzyme produced oligosaccharides together with galactose, suggesting that it is able to bypass beta-1,6-linked galactosyl side chains. |
T10 | 1683-1883 | Sentence | denotes | The C-terminal CBM6 did not show any affinity for known substrates of CBM6 such as xylan, cellulose, and beta-1,3-glucan, although it bound beta-1,3-galactan when analyzed by affinity electrophoresis. |
T11 | 1884-2142 | Sentence | denotes | Frontal affinity chromatography for the CBM6 moiety using several kinds of terminal galactose-containing oligosaccharides as the analytes clearly indicated that the CBM6 specifically interacted with oligosaccharides containing a beta-1,3-galactobiose moiety. |
T12 | 2143-2272 | Sentence | denotes | When the degree of polymerization of galactose oligomers was increased, the binding affinity of the CBM6 showed no marked change. |
GlyCosmos15-Glycan
Id | Subject | Object | Predicate | Lexical cue | image |
---|---|---|---|---|---|
T1 | 898-906 | Glycan | denotes | arabinan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G22535ZZ |
T2 | 931-939 | Glycan | denotes | arabinan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G22535ZZ |
GlyCosmos15-GlycoEpitope
Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
---|---|---|---|---|---|
T1 | 898-906 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | arabinan | http://www.glycoepitope.jp/epitopes/EP0508 |
T2 | 931-939 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | arabinan | http://www.glycoepitope.jp/epitopes/EP0508 |
NCBITAXON
Id | Subject | Object | Predicate | Lexical cue | db_id |
---|---|---|---|---|---|
T1 | 81-108 | OrganismTaxon | denotes | Phanerochaete chrysosporium | 2822231 |
T2 | 148-175 | OrganismTaxon | denotes | Phanerochaete chrysosporium | 2822231 |
T3 | 221-236 | OrganismTaxon | denotes | Pichia pastoris | 4922 |
T4 | 908-918 | OrganismTaxon | denotes | gum arabic | 138043 |
Anatomy-UBERON
Id | Subject | Object | Predicate | Lexical cue | uberon_id |
---|---|---|---|---|---|
T1 | 908-911 | Body_part | denotes | gum | http://purl.obolibrary.org/obo/UBERON_0001828 |
T2 | 1884-1891 | Body_part | denotes | Frontal | http://purl.obolibrary.org/obo/UBERON_0000209 |