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PubMed:15818704 JSONTXT

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DisGeNET

Id Subject Object Predicate Lexical cue
T0 669-675 gene:8751 denotes ADAM15
T1 780-790 disease:C0015302 denotes osteophyte
T2 669-675 gene:8751 denotes ADAM15
T3 780-790 disease:C1956089 denotes osteophyte
R1 T0 T1 associated_with ADAM15,osteophyte
R2 T2 T3 associated_with ADAM15,osteophyte

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
15818704-10#56#62#gene8751 1822-1828 gene8751 denotes ADAM15
15818704-10#148#154#gene8751 1914-1920 gene8751 denotes ADAM15
15818704-10#226#232#gene8751 1992-1998 gene8751 denotes ADAM15
15818704-10#38#52#diseaseC0029408 1804-1818 diseaseC0029408 denotes osteoarthritis
15818704-4#53#59#gene8751 669-675 gene8751 denotes ADAM15
15818704-4#164#174#diseaseC0015302 780-790 diseaseC0015302 denotes osteophyte
15818704-4#164#174#diseaseC1956089 780-790 diseaseC1956089 denotes osteophyte
56#62#gene875138#52#diseaseC0029408 15818704-10#56#62#gene8751 15818704-10#38#52#diseaseC0029408 associated_with ADAM15,osteoarthritis
148#154#gene875138#52#diseaseC0029408 15818704-10#148#154#gene8751 15818704-10#38#52#diseaseC0029408 associated_with ADAM15,osteoarthritis
226#232#gene875138#52#diseaseC0029408 15818704-10#226#232#gene8751 15818704-10#38#52#diseaseC0029408 associated_with ADAM15,osteoarthritis
53#59#gene8751164#174#diseaseC0015302 15818704-4#53#59#gene8751 15818704-4#164#174#diseaseC0015302 associated_with ADAM15,osteophyte
53#59#gene8751164#174#diseaseC1956089 15818704-4#53#59#gene8751 15818704-4#164#174#diseaseC1956089 associated_with ADAM15,osteophyte

PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 113-606 OBJECTIVE denotes The membrane-anchored metalloproteinase disintegrin ADAM15 is up-regulated in osteoarthritis and has been implicated in proteolysis and cell-matrix interactions. To address its role in cartilage metabolism, we performed an analysis of joint morphology in aging mice with a targeted inactivation of the ADAM15 gene (ADAM15(-/-)). In addition, a human chondrocyte cell line overexpressing ADAM15 was used to investigate the role of ADAM15 in an in vitro model of chondrocyte-matrix interactions.
T2 616-1270 METHODS denotes Knee joint sections from 3-, 6-, and 12-14-month-old ADAM15(-/-) and wild-type (WT) 129/SvJ mice were examined for synovial hyperplasia, cartilage degradation, and osteophyte formation. Stable transfection of the human T/C28a4 chondrocyte cell line with full-length human ADAM15 complementary DNA led to the establishment of ADAM15-overexpressing chondrocytes that were further analyzed for their capability to adhere to and to survive on cartilage matrix molecules (fibronectin and types II and VI collagen) under conditions of serum starvation. ADAM15 expression was investigated by reverse transcription-polymerase chain reaction and Western blotting.
T3 1280-1753 RESULTS denotes Aging ADAM15(-/-) mice exhibited accelerated development of osteoarthritic lesions compared with WT mice, and the difference was statistically significant at age 12 months. The osteoarthritic changes preferentially affected male ADAM15(-/-) mice. ADAM15 overexpression in T/C28a4 cells led to the specific reinforcement of chondrocyte adhesion to cartilage types II and VI collagen, and this was associated with enhanced cell viability under conditions of serum starvation.
T4 1766-2023 CONCLUSIONS denotes The accelerated development of murine osteoarthritis in ADAM15 deficiency as well as the proadhesive and cell survival-promoting in vitro effect of ADAM15 overexpression suggest a homeostatic rather than a destructive role of ADAM15 in cartilage remodeling.