| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-58 |
Sentence |
denotes |
Endo180 binds to the C-terminal region of type I collagen. |
| T2 |
59-227 |
Sentence |
denotes |
Type I collagen is a fibril-forming heterotrimer composed of two alpha1 and one alpha2 chains and plays a crucial role in cell-matrix adhesion and cell differentiation. |
| T3 |
228-478 |
Sentence |
denotes |
Through a comprehensive differential display screening of oncogenic ras target genes, we have shown that the alpha1 chain of type I collagen (col1a1) is markedly down-regulated by the ras oncogene through the mitogen-activated protein kinase pathway. |
| T4 |
479-701 |
Sentence |
denotes |
Although ras-transformed cells are no longer able to produce and secrete endogenous collagen, they can still adhere to exogenous collagen, suggesting that the cells express a collagen binding factor(s) on the cell surface. |
| T5 |
702-963 |
Sentence |
denotes |
When the region of col1a1 encompassing the C-terminal glycine repeat and C-prodomain (amino acids 1000-1453) was affinity-labeled with human placental alkaline phosphatase, the secreted trimeric fusion protein could bind to the surface of Ras-transformed cells. |
| T6 |
964-1190 |
Sentence |
denotes |
Using biochemical purification followed by matrix-assisted laser desorption/ionization mass spectrometry analysis, we identified this collagen binding factor as Endo180 (uPARAP, CD280), a member of the mannose receptor family. |
| T7 |
1191-1382 |
Sentence |
denotes |
Ectopic expression of Endo180 in CosE5 cells followed by in situ staining and quantitative binding assays confirmed that Endo180 indeed recognizes and binds to placental alkaline phosphatase. |
| T8 |
1383-1518 |
Sentence |
denotes |
The interaction between Endo180 and the C-terminal region of type I collagen appears to play an important role in cell-matrix adhesion. |
