PubMed:15748736 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/15748736","sourcedb":"PubMed","sourceid":"15748736","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/15748736","text":"Reexamination of phenoloxidase in larval circulating hemocytes of the silkworm, Bombyx mori.\nWe have developed a modified method to detect phenoloxidase activity on hemocytes by using freshly prepared l-DOPA (1 mg/ml in 35% ethanol) to fix and incubate larval hemocytes. This method is more sensitive than the common method, in which hemocytes were fixed in 4% formaldehyde and then incubated with 2 mg/ml l-DOPA in water separately. Phenoloxidase assayed using this modified method can be inhibited by phenyltiourea (phenoloxidase inhibitor). After incubation with l-DOPA solution in ethanol, most prohemocytes, all plasmatocytes and young granulocytes are stained brown due to oxidation of l-DOPA into pigments, indicating that they have phenoloxidase. Oenocytoids are dimly stained because many of their cell inclusions have been released during the treatment. Large propidium-iodide-negative prohemocytes have strong phenoloxidase activity and are easily misunderstood as propidium-iodide-positive oenocytoids if the fluorescent method is not used for identification. Thus, in addition to oenocytoids and plasmatocytes, some prohemocytes and granulocytes in the silkworm also have phenoloxidase.","tracks":[{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":70,"end":78},"obj":"Species:7091"},{"id":"T2","span":{"begin":80,"end":91},"obj":"Species:7091"},{"id":"T3","span":{"begin":201,"end":207},"obj":"Chemical:MESH:D007980"},{"id":"T4","span":{"begin":224,"end":231},"obj":"Chemical:MESH:D000431"},{"id":"T5","span":{"begin":361,"end":373},"obj":"Chemical:MESH:D005557"},{"id":"T6","span":{"begin":406,"end":412},"obj":"Chemical:MESH:D007980"},{"id":"T7","span":{"begin":416,"end":421},"obj":"Chemical:MESH:D014867"},{"id":"T8","span":{"begin":503,"end":516},"obj":"Chemical:-"},{"id":"T9","span":{"begin":566,"end":572},"obj":"Chemical:MESH:D007980"},{"id":"T10","span":{"begin":585,"end":592},"obj":"Chemical:MESH:D000431"},{"id":"T11","span":{"begin":692,"end":698},"obj":"Chemical:MESH:D007980"},{"id":"T12","span":{"begin":870,"end":886},"obj":"Chemical:MESH:D011419"},{"id":"T13","span":{"begin":976,"end":992},"obj":"Chemical:MESH:D011419"},{"id":"T14","span":{"begin":1166,"end":1174},"obj":"Species:7091"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkworm"},{"subj":"T2","pred":"source","obj":"silkworm"},{"subj":"T3","pred":"source","obj":"silkworm"},{"subj":"T4","pred":"source","obj":"silkworm"},{"subj":"T5","pred":"source","obj":"silkworm"},{"subj":"T6","pred":"source","obj":"silkworm"},{"subj":"T7","pred":"source","obj":"silkworm"},{"subj":"T8","pred":"source","obj":"silkworm"},{"subj":"T9","pred":"source","obj":"silkworm"},{"subj":"T10","pred":"source","obj":"silkworm"},{"subj":"T11","pred":"source","obj":"silkworm"},{"subj":"T12","pred":"source","obj":"silkworm"},{"subj":"T13","pred":"source","obj":"silkworm"},{"subj":"T14","pred":"source","obj":"silkworm"}]},{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":70,"end":78},"obj":"Species:7091"},{"id":"T10","span":{"begin":585,"end":592},"obj":"Chemical:MESH:D000431"},{"id":"T11","span":{"begin":692,"end":698},"obj":"Chemical:MESH:D007980"},{"id":"T12","span":{"begin":870,"end":886},"obj":"Chemical:MESH:D011419"},{"id":"T13","span":{"begin":976,"end":992},"obj":"Chemical:MESH:D011419"},{"id":"T14","span":{"begin":1166,"end":1174},"obj":"Species:7091"},{"id":"T2","span":{"begin":80,"end":91},"obj":"Species:7091"},{"id":"T3","span":{"begin":201,"end":207},"obj":"Chemical:MESH:D007980"},{"id":"T4","span":{"begin":224,"end":231},"obj":"Chemical:MESH:D000431"},{"id":"T5","span":{"begin":361,"end":373},"obj":"Chemical:MESH:D005557"},{"id":"T6","span":{"begin":406,"end":412},"obj":"Chemical:MESH:D007980"},{"id":"T7","span":{"begin":416,"end":421},"obj":"Chemical:MESH:D014867"},{"id":"T8","span":{"begin":503,"end":516},"obj":"Chemical:-"},{"id":"T9","span":{"begin":566,"end":572},"obj":"Chemical:MESH:D007980"},{"id":"T18","span":{"begin":666,"end":671},"obj":"BMO_00497"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkwormbase"},{"subj":"T10","pred":"source","obj":"silkwormbase"},{"subj":"T11","pred":"source","obj":"silkwormbase"},{"subj":"T12","pred":"source","obj":"silkwormbase"},{"subj":"T13","pred":"source","obj":"silkwormbase"},{"subj":"T14","pred":"source","obj":"silkwormbase"},{"subj":"T2","pred":"source","obj":"silkwormbase"},{"subj":"T3","pred":"source","obj":"silkwormbase"},{"subj":"T4","pred":"source","obj":"silkwormbase"},{"subj":"T5","pred":"source","obj":"silkwormbase"},{"subj":"T6","pred":"source","obj":"silkwormbase"},{"subj":"T7","pred":"source","obj":"silkwormbase"},{"subj":"T8","pred":"source","obj":"silkwormbase"},{"subj":"T9","pred":"source","obj":"silkwormbase"},{"subj":"T18","pred":"source","obj":"silkwormbase"}]},{"project":"silkworm_phenotype","denotations":[{"id":"T18","span":{"begin":666,"end":671},"obj":"BMO_00497"},{"id":"T9","span":{"begin":566,"end":572},"obj":"Chemical:MESH:D007980"},{"id":"T8","span":{"begin":503,"end":516},"obj":"Chemical:-"},{"id":"T7","span":{"begin":416,"end":421},"obj":"Chemical:MESH:D014867"},{"id":"T6","span":{"begin":406,"end":412},"obj":"Chemical:MESH:D007980"},{"id":"T5","span":{"begin":361,"end":373},"obj":"Chemical:MESH:D005557"},{"id":"T4","span":{"begin":224,"end":231},"obj":"Chemical:MESH:D000431"},{"id":"T3","span":{"begin":201,"end":207},"obj":"Chemical:MESH:D007980"},{"id":"T2","span":{"begin":80,"end":91},"obj":"Species:7091"},{"id":"T14","span":{"begin":1166,"end":1174},"obj":"Species:7091"},{"id":"T13","span":{"begin":976,"end":992},"obj":"Chemical:MESH:D011419"},{"id":"T12","span":{"begin":870,"end":886},"obj":"Chemical:MESH:D011419"},{"id":"T11","span":{"begin":692,"end":698},"obj":"Chemical:MESH:D007980"},{"id":"T10","span":{"begin":585,"end":592},"obj":"Chemical:MESH:D000431"},{"id":"T1","span":{"begin":70,"end":78},"obj":"Species:7091"}],"attributes":[{"subj":"T18","pred":"source","obj":"silkworm_phenotype"},{"subj":"T9","pred":"source","obj":"silkworm_phenotype"},{"subj":"T8","pred":"source","obj":"silkworm_phenotype"},{"subj":"T7","pred":"source","obj":"silkworm_phenotype"},{"subj":"T6","pred":"source","obj":"silkworm_phenoty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