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PubMed:15718492 JSONTXT

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DisGeNET

Id Subject Object Predicate Lexical cue
T0 303-306 gene:54808 denotes SMC
T1 366-372 disease:C3825627 denotes stress
R1 T0 T1 associated_with SMC,stress

PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 131-373 OBJECTIVE denotes Vascular endothelial cells (ECs) are influenced by shear stress and neighboring smooth muscle cells (SMCs). We investigated the inflammation-relevant gene expression in EC/SMC cocultures under static condition and in response to shear stress.
T2 397-1416 METHODS denotes Under static condition, DNA microarrays and reverse-transcription polymerase chain reaction identified 23 inflammation-relevant genes in ECs whose expression was significantly affected by coculture with SMCs, with 18 upregulated and 5 downregulated. Application of shear stress (12 dynes/cm2) to the EC side of the coculture for 6 hours inhibited most of the proinflammatory gene expressions in ECs induced by coculture with SMCs. Inhibition of nuclear factor-kappaB (NF-kappaB) activation by the p65-antisense, lactacystin, and N-acetyl-cysteine blocked the coculture-induced EC expression of proinflammatory genes, indicating that the NF-kappaB binding sites in the promoters of these genes play a significant role in their expression as a result of coculture with SMCs. Chromatin immunoprecipitation assays demonstrated the in vivo regulation of NF-kappaB recruitment to selected target promoters. Shear stress inhibited the SMC coculture-induced NF-kappaB activation in ECs and monocytic THP-1 cell adhesion to ECs.
T3 1430-1575 CONCLUSIONS denotes Our findings suggest that shear stress plays an inhibitory role in the proinflammatory gene expression in ECs located in close proximity to SMCs.

Inflammaging

Id Subject Object Predicate Lexical cue
T1 0-118 Sentence denotes Shear stress inhibits smooth muscle cell-induced inflammatory gene expression in endothelial cells: role of NF-kappaB.
T2 119-130 Sentence denotes OBJECTIVES:
T3 131-238 Sentence denotes Vascular endothelial cells (ECs) are influenced by shear stress and neighboring smooth muscle cells (SMCs).
T4 239-373 Sentence denotes We investigated the inflammation-relevant gene expression in EC/SMC cocultures under static condition and in response to shear stress.
T5 374-396 Sentence denotes MATERIALS AND METHODS:
T6 397-646 Sentence denotes Under static condition, DNA microarrays and reverse-transcription polymerase chain reaction identified 23 inflammation-relevant genes in ECs whose expression was significantly affected by coculture with SMCs, with 18 upregulated and 5 downregulated.
T7 647-827 Sentence denotes Application of shear stress (12 dynes/cm2) to the EC side of the coculture for 6 hours inhibited most of the proinflammatory gene expressions in ECs induced by coculture with SMCs.
T8 828-1169 Sentence denotes Inhibition of nuclear factor-kappaB (NF-kappaB) activation by the p65-antisense, lactacystin, and N-acetyl-cysteine blocked the coculture-induced EC expression of proinflammatory genes, indicating that the NF-kappaB binding sites in the promoters of these genes play a significant role in their expression as a result of coculture with SMCs.
T9 1170-1297 Sentence denotes Chromatin immunoprecipitation assays demonstrated the in vivo regulation of NF-kappaB recruitment to selected target promoters.
T10 1298-1416 Sentence denotes Shear stress inhibited the SMC coculture-induced NF-kappaB activation in ECs and monocytic THP-1 cell adhesion to ECs.
T11 1417-1429 Sentence denotes CONCLUSIONS:
T12 1430-1575 Sentence denotes Our findings suggest that shear stress plays an inhibitory role in the proinflammatory gene expression in ECs located in close proximity to SMCs.
T1 0-118 Sentence denotes Shear stress inhibits smooth muscle cell-induced inflammatory gene expression in endothelial cells: role of NF-kappaB.
T2 119-130 Sentence denotes OBJECTIVES:
T3 131-238 Sentence denotes Vascular endothelial cells (ECs) are influenced by shear stress and neighboring smooth muscle cells (SMCs).
T4 239-373 Sentence denotes We investigated the inflammation-relevant gene expression in EC/SMC cocultures under static condition and in response to shear stress.
T5 374-396 Sentence denotes MATERIALS AND METHODS:
T6 397-646 Sentence denotes Under static condition, DNA microarrays and reverse-transcription polymerase chain reaction identified 23 inflammation-relevant genes in ECs whose expression was significantly affected by coculture with SMCs, with 18 upregulated and 5 downregulated.
T7 647-827 Sentence denotes Application of shear stress (12 dynes/cm2) to the EC side of the coculture for 6 hours inhibited most of the proinflammatory gene expressions in ECs induced by coculture with SMCs.
T8 828-1169 Sentence denotes Inhibition of nuclear factor-kappaB (NF-kappaB) activation by the p65-antisense, lactacystin, and N-acetyl-cysteine blocked the coculture-induced EC expression of proinflammatory genes, indicating that the NF-kappaB binding sites in the promoters of these genes play a significant role in their expression as a result of coculture with SMCs.
T9 1170-1297 Sentence denotes Chromatin immunoprecipitation assays demonstrated the in vivo regulation of NF-kappaB recruitment to selected target promoters.
T10 1298-1416 Sentence denotes Shear stress inhibited the SMC coculture-induced NF-kappaB activation in ECs and monocytic THP-1 cell adhesion to ECs.
T11 1417-1429 Sentence denotes CONCLUSIONS:
T12 1430-1575 Sentence denotes Our findings suggest that shear stress plays an inhibitory role in the proinflammatory gene expression in ECs located in close proximity to SMCs.