Id |
Subject |
Object |
Predicate |
Lexical cue |
TextSentencer_T1 |
0-250 |
Sentence |
denotes |
Selective cleavage and anticoagulant activity of a sulfated fucan: stereospecific removal of a 2-sulfate ester from the polysaccharide by mild acid hydrolysis, preparation of oligosaccharides, and heparin cofactor II-dependent anticoagulant activity. |
TextSentencer_T2 |
251-531 |
Sentence |
denotes |
A linear sulfated fucan with a regular repeating sequence of [3)-alpha-L-Fucp-(2SO4)-(1-->3)-alpha-L-Fucp-(4SO4)-(1-->3)-alpha-L-Fucp-(2,4SO4)-(1-->3)-alpha-L-Fucp-(2SO4)-(1-->]n is an anticoagulant polysaccharide mainly due to thrombin inhibition mediated by heparin cofactor II. |
TextSentencer_T3 |
532-656 |
Sentence |
denotes |
No specific enzymatic or chemical method is available for the preparation of tailored oligosaccharides from sulfated fucans. |
TextSentencer_T4 |
657-767 |
Sentence |
denotes |
We employ an apparently nonspecific approach to cleave this polysaccharide based on mild hydrolysis with acid. |
TextSentencer_T5 |
768-867 |
Sentence |
denotes |
Surprisingly, the linear sulfated fucan was cleaved by mild acid hydrolysis on an ordered sequence. |
TextSentencer_T6 |
868-944 |
Sentence |
denotes |
Initially a 2-sulfate ester of the first fucose unit is selectively removed. |
TextSentencer_T7 |
945-1150 |
Sentence |
denotes |
Thereafter the glycosidic linkage between the nonsulfated fucose residue and the subsequent 4-sulfated residue is preferentially cleaved by acid hydrolysis, forming oligosaccharides with well-defined size. |
TextSentencer_T8 |
1151-1372 |
Sentence |
denotes |
The low-molecular-weight derivatives obtained from the sulfated fucan were employed to determine the requirement for interaction of this polysaccharide with heparin cofactor II and to achieve complete thrombin inhibition. |
TextSentencer_T9 |
1373-1504 |
Sentence |
denotes |
The linear sulfated fucan requires significantly longer chains than mammalian glycosaminoglycans to achieve anticoagulant activity. |
TextSentencer_T10 |
1505-1656 |
Sentence |
denotes |
A slight decrease in the molecular size of the sulfated fucan dramatically reduces its effect on thrombin inactivation mediated by heparin cofactor II. |
TextSentencer_T11 |
1657-1823 |
Sentence |
denotes |
Sulfated fucan with approximately 45 tetrasaccharide repeating units binds to heparin cofactor II but is unable to link efficiently the plasma inhibitor and thrombin. |
TextSentencer_T12 |
1824-1920 |
Sentence |
denotes |
This last effect requires chains with approximately 100 or more tetrasaccharide repeating units. |
TextSentencer_T13 |
1921-2110 |
Sentence |
denotes |
We speculate that the template mechanism may predominate over the allosteric effect in the case of the linear sulfated fucan inactivation of thrombin in the presence of heparin cofactor II. |
T1 |
0-250 |
Sentence |
denotes |
Selective cleavage and anticoagulant activity of a sulfated fucan: stereospecific removal of a 2-sulfate ester from the polysaccharide by mild acid hydrolysis, preparation of oligosaccharides, and heparin cofactor II-dependent anticoagulant activity. |
T2 |
251-531 |
Sentence |
denotes |
A linear sulfated fucan with a regular repeating sequence of [3)-alpha-L-Fucp-(2SO4)-(1-->3)-alpha-L-Fucp-(4SO4)-(1-->3)-alpha-L-Fucp-(2,4SO4)-(1-->3)-alpha-L-Fucp-(2SO4)-(1-->]n is an anticoagulant polysaccharide mainly due to thrombin inhibition mediated by heparin cofactor II. |
T3 |
532-656 |
Sentence |
denotes |
No specific enzymatic or chemical method is available for the preparation of tailored oligosaccharides from sulfated fucans. |
T4 |
657-767 |
Sentence |
denotes |
We employ an apparently nonspecific approach to cleave this polysaccharide based on mild hydrolysis with acid. |
T5 |
768-867 |
Sentence |
denotes |
Surprisingly, the linear sulfated fucan was cleaved by mild acid hydrolysis on an ordered sequence. |
T6 |
868-944 |
Sentence |
denotes |
Initially a 2-sulfate ester of the first fucose unit is selectively removed. |
T7 |
945-1150 |
Sentence |
denotes |
Thereafter the glycosidic linkage between the nonsulfated fucose residue and the subsequent 4-sulfated residue is preferentially cleaved by acid hydrolysis, forming oligosaccharides with well-defined size. |
T8 |
1151-1372 |
Sentence |
denotes |
The low-molecular-weight derivatives obtained from the sulfated fucan were employed to determine the requirement for interaction of this polysaccharide with heparin cofactor II and to achieve complete thrombin inhibition. |
T9 |
1373-1504 |
Sentence |
denotes |
The linear sulfated fucan requires significantly longer chains than mammalian glycosaminoglycans to achieve anticoagulant activity. |
T10 |
1505-1656 |
Sentence |
denotes |
A slight decrease in the molecular size of the sulfated fucan dramatically reduces its effect on thrombin inactivation mediated by heparin cofactor II. |
T11 |
1657-1823 |
Sentence |
denotes |
Sulfated fucan with approximately 45 tetrasaccharide repeating units binds to heparin cofactor II but is unable to link efficiently the plasma inhibitor and thrombin. |
T12 |
1824-1920 |
Sentence |
denotes |
This last effect requires chains with approximately 100 or more tetrasaccharide repeating units. |
T13 |
1921-2110 |
Sentence |
denotes |
We speculate that the template mechanism may predominate over the allosteric effect in the case of the linear sulfated fucan inactivation of thrombin in the presence of heparin cofactor II. |
T1 |
0-250 |
Sentence |
denotes |
Selective cleavage and anticoagulant activity of a sulfated fucan: stereospecific removal of a 2-sulfate ester from the polysaccharide by mild acid hydrolysis, preparation of oligosaccharides, and heparin cofactor II-dependent anticoagulant activity. |
T2 |
251-531 |
Sentence |
denotes |
A linear sulfated fucan with a regular repeating sequence of [3)-alpha-L-Fucp-(2SO4)-(1-->3)-alpha-L-Fucp-(4SO4)-(1-->3)-alpha-L-Fucp-(2,4SO4)-(1-->3)-alpha-L-Fucp-(2SO4)-(1-->]n is an anticoagulant polysaccharide mainly due to thrombin inhibition mediated by heparin cofactor II. |
T3 |
532-656 |
Sentence |
denotes |
No specific enzymatic or chemical method is available for the preparation of tailored oligosaccharides from sulfated fucans. |
T4 |
657-767 |
Sentence |
denotes |
We employ an apparently nonspecific approach to cleave this polysaccharide based on mild hydrolysis with acid. |
T5 |
768-867 |
Sentence |
denotes |
Surprisingly, the linear sulfated fucan was cleaved by mild acid hydrolysis on an ordered sequence. |
T6 |
868-944 |
Sentence |
denotes |
Initially a 2-sulfate ester of the first fucose unit is selectively removed. |
T7 |
945-1150 |
Sentence |
denotes |
Thereafter the glycosidic linkage between the nonsulfated fucose residue and the subsequent 4-sulfated residue is preferentially cleaved by acid hydrolysis, forming oligosaccharides with well-defined size. |
T8 |
1151-1372 |
Sentence |
denotes |
The low-molecular-weight derivatives obtained from the sulfated fucan were employed to determine the requirement for interaction of this polysaccharide with heparin cofactor II and to achieve complete thrombin inhibition. |
T9 |
1373-1504 |
Sentence |
denotes |
The linear sulfated fucan requires significantly longer chains than mammalian glycosaminoglycans to achieve anticoagulant activity. |
T10 |
1505-1656 |
Sentence |
denotes |
A slight decrease in the molecular size of the sulfated fucan dramatically reduces its effect on thrombin inactivation mediated by heparin cofactor II. |
T11 |
1657-1823 |
Sentence |
denotes |
Sulfated fucan with approximately 45 tetrasaccharide repeating units binds to heparin cofactor II but is unable to link efficiently the plasma inhibitor and thrombin. |
T12 |
1824-1920 |
Sentence |
denotes |
This last effect requires chains with approximately 100 or more tetrasaccharide repeating units. |
T13 |
1921-2110 |
Sentence |
denotes |
We speculate that the template mechanism may predominate over the allosteric effect in the case of the linear sulfated fucan inactivation of thrombin in the presence of heparin cofactor II. |