PubMed:1551863
Annnotations
{"target":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/1551863","sourcedb":"PubMed","sourceid":"1551863","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1551863","text":"Enhanced CO2/O2 specificity of a site-directed mutant of ribulose-bisphosphate carboxylase/oxygenase.\nThe CO2/O2 specificity factor of ribulose-bisphosphate carboxylase/oxygenase partially determines the efficiency of photosynthetic carbon assimilation. Heretofore, engineered alterations of the enzyme have only decreased the selectivity for CO2 utilization. We show that alanyl replacement of active-site Ser-368 of the Rhodospirillum rubrum carboxylase enhances the carboxylation selectivity approximately 1.6-fold over the wild-type level. This enhancement reflects a greater relative decline in oxygenase efficiency than in carboxylase efficiency. In contrast to wild-type enzyme, the carboxylase activity of the Ser-368 mutant protein is not perceptibly inhibited by O2, perhaps indicative of a change in rate-limiting steps in the overall reaction pathway.","tracks":[{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":101},"obj":"Sentence"},{"id":"T2","span":{"begin":102,"end":253},"obj":"Sentence"},{"id":"T3","span":{"begin":254,"end":359},"obj":"Sentence"},{"id":"T4","span":{"begin":360,"end":543},"obj":"Sentence"},{"id":"T5","span":{"begin":544,"end":652},"obj":"Sentence"},{"id":"T6","span":{"begin":653,"end":863},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":101},"obj":"Sentence"},{"id":"T2","span":{"begin":102,"end":253},"obj":"Sentence"},{"id":"T3","span":{"begin":254,"end":359},"obj":"Sentence"},{"id":"T4","span":{"begin":360,"end":543},"obj":"Sentence"},{"id":"T5","span":{"begin":544,"end":652},"obj":"Sentence"},{"id":"T6","span":{"begin":653,"end":863},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":422,"end":443},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"NCBItxid:1085"},{"subj":"T1","pred":"source","obj":"NCBITAXON"}]},{"project":"GlyCosmos6-CLO","denotations":[{"id":"T1","span":{"begin":395,"end":401},"obj":"http://purl.obolibrary.org/obo/CLO_0001658"},{"id":"T2","span":{"begin":702,"end":710},"obj":"http://purl.obolibrary.org/obo/CLO_0001658"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-CLO"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-CLO"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#c693ec","default":true},{"id":"NCBITAXON","color":"#93ecac"},{"id":"GlyCosmos6-CLO","color":"#ec9393"}]}]}}