PubMed:1535553 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/1535553","sourcedb":"PubMed","sourceid":"1535553","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1535553","text":"Combined chemical-enzymic synthesis of a dideoxypentasaccharide for use in a study of the specificity of N-acetyl-glucosaminyltransferase-III.\nThe biantennary oligosaccharide glycoside beta-D-GlcpNAc-(1----2)-alpha-D- Manp-(1----3)- [beta-D-GlcpNAc-(1----2)-alpha-D-Manp-(1----6)]-beta-D-Manp- OR is a potential substrate for N-acetylglucosaminyltransferases (GlcNAcTs) III-V. The dideoxypentasaccharide glycoside beta-D-GlcpNAc-(1----2)-4- deoxy-alpha-D-lyxo-Hexp-(1----3)- [beta-DGlcpNAc-(1----2)-6-deoxy-alpha-D-Manp-(1----6)] beta-D-Manp-O(CH2)7CH3 (5), where the hydroxyl groups that would be acted on by GlcNAcTs IV and V have been removed, was prepared as a possible specific acceptor for GlcNAcT-III. The strategy involved the chemical synthesis of beta-D-GlcpNAc-(1----2)-4-deoxy-alpha-D-lyxo-Hexp-(1----3)-] 6- deoxy-alpha-D-Manp-(1----6)]-beta-D-Manp-O)CH2)7CH3 and then addition of the last GlcpNAc residue using partially purified GlcNAcT-II from rabbit liver. Preliminary results, using detergent extracts from rat kidney, indicate that 5 is an acceptor for a GlcNAcT whose identity remains to be established.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":142},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":143,"end":376},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":377,"end":708},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":709,"end":973},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":974,"end":1123},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":142},"obj":"Sentence"},{"id":"T2","span":{"begin":143,"end":376},"obj":"Sentence"},{"id":"T3","span":{"begin":377,"end":708},"obj":"Sentence"},{"id":"T4","span":{"begin":709,"end":973},"obj":"Sentence"},{"id":"T5","span":{"begin":974,"end":1123},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"}]},{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":967,"end":972},"obj":"http://purl.obolibrary.org/obo/MAT_0000097"},{"id":"T2","span":{"begin":1029,"end":1035},"obj":"http://purl.obolibrary.org/obo/MAT_0000119"}],"attributes":[{"subj":"T1","pred":"source","obj":"Glycosmos6-MAT"},{"subj":"T2","pred":"source","obj":"Glycosmos6-MAT"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":1025,"end":1028},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"10114"},{"id":"A2","pred":"db_id","subj":"T1","obj":"10116"},{"subj":"T1","pred":"source","obj":"NCBITAXON"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":967,"end":972},"obj":"Body_part"},{"id":"T2","span":{"begin":1029,"end":1035},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0002107"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0002113"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T2","pred":"source","obj":"Anatomy-UBERON"}]},{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":967,"end":972},"obj":"Body_part"},{"id":"T2","span":{"begin":1029,"end":1035},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000097"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000119"},{"subj":"T1","pred":"source","obj":"Anatomy-MAT"},{"subj":"T2","pred":"source","obj":"Anatomy-MAT"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#9398ec","default":true},{"id":"Glycosmos6-MAT","color":"#b2ec93"},{"id":"NCBITAXON","color":"#ec93cc"},{"id":"Anatomy-UBERON","color":"#93e6ec"},{"id":"Anatomy-MAT","color":"#ecd793"}]}]}}