PubMed:15355234 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/15355234","sourcedb":"PubMed","sourceid":"15355234","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/15355234","text":"The genetics and genomics of the silkworm, Bombyx mori.\nWe review progress in applying molecular genetic and genomic technologies to studies in the domesticated silkworm, Bombyx mori, highlighting its use as a model for Lepidoptera, and in sericulture and biotechnology. Dense molecular linkage maps are being integrated with classical linkage maps for positional cloning and marker-assisted selection. Classical mutations have been identified by a candidate gene approach. Cytogenetic and sequence analyses show that the W chromosome is composed largely of nested full-length long terminal repeat retrotransposons. Z-chromosome-linked sequences show a lack of dosage compensation. The downstream sex differentiation mechanism has been studied via the silkworm homolog of doublesex. Expressed sequence tagged databases have been used to discover Lepidoptera-specific genes, provide evidence for horizontal gene transfer, and construct microarrays. Physical maps using large-fragment bacterial artificial chromosome libraries have been constructed, and whole-genome shotgun sequencing is underway. Germline transformation and transient expression systems are well established and available for functional studies, high-level protein expression, and gene silencing via RNA interference.","tracks":[{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":33,"end":41},"obj":"Species:7091"},{"id":"T2","span":{"begin":43,"end":54},"obj":"Species:7091"},{"id":"T3","span":{"begin":161,"end":169},"obj":"Species:7091"},{"id":"T4","span":{"begin":171,"end":182},"obj":"Species:7091"},{"id":"T5","span":{"begin":752,"end":760},"obj":"Species:7091"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkworm"},{"subj":"T2","pred":"source","obj":"silkworm"},{"subj":"T3","pred":"source","obj":"silkworm"},{"subj":"T4","pred":"source","obj":"silkworm"},{"subj":"T5","pred":"source","obj":"silkworm"}]},{"project":"silkwormbase","denotations":[{"id":"T5","span":{"begin":752,"end":760},"obj":"Species:7091"},{"id":"T4","span":{"begin":171,"end":182},"obj":"Species:7091"},{"id":"T3","span":{"begin":161,"end":169},"obj":"Species:7091"},{"id":"T2","span":{"begin":43,"end":54},"obj":"Species:7091"},{"id":"T1","span":{"begin":33,"end":41},"obj":"Species:7091"}],"attributes":[{"subj":"T5","pred":"source","obj":"silkwormbase"},{"subj":"T4","pred":"source","obj":"silkwormbase"},{"subj":"T3","pred":"source","obj":"silkwormbase"},{"subj":"T2","pred":"source","obj":"silkwormbase"},{"subj":"T1","pred":"source","obj":"silkwormbase"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"silkworm","color":"#ab93ec","default":true},{"id":"silkwormbase","color":"#94ec93"}]}]}}