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GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 0-11 Glycan_Motif denotes Sialic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T2 163-174 Glycan_Motif denotes sialic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T3 216-219 Glycan_Motif denotes GM1 https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G48558GR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46613JI
T5 669-692 Glycan_Motif denotes N-acetylneuraminic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G50850NI
T7 694-700 Glycan_Motif denotes Neu5Ac https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T8 864-875 Glycan_Motif denotes sialic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T9 1222-1233 Glycan_Motif denotes sialic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T10 1373-1396 Glycan_Motif denotes N-acetylneuraminic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G50850NI
T12 1591-1602 Glycan_Motif denotes sialic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T13 1689-1700 Glycan_Motif denotes sialic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY

sentences

Id Subject Object Predicate Lexical cue
T1 0-57 Sentence denotes Sialic acid recognition by Vibrio cholerae neuraminidase.
T2 58-252 Sentence denotes Vibrio cholerae neuraminidase (VCNA) plays a significant role in the pathogenesis of cholera by removing sialic acid from higher order gangliosides to unmask GM1, the receptor for cholera toxin.
T3 253-490 Sentence denotes We previously showed that the structure of VCNA is composed of a central beta-propeller catalytic domain flanked by two lectin-like domains; however the nature of the carbohydrates recognized by these lectin domains has remained unknown.
T4 491-616 Sentence denotes We present here structures of the enzyme in complex with two substrates, alpha-2,3-sialyllactose and alpha-2,6-sialyllactose.
T5 617-783 Sentence denotes Both substrate complexes reveal the alpha-anomer of N-acetylneuraminic acid (Neu5Ac) bound to the N-terminal lectin domain, thereby revealing the role of this domain.
T6 784-953 Sentence denotes The large number of interactions suggest a relatively high binding affinity for sialic acid, which was confirmed by calorimetry, which gave a Kd approximately 30 microm.
T7 954-1143 Sentence denotes Saturation transfer difference NMR using a non-hydrolyzable substrate, Neu5,9Ac2-2-S-(alpha-2,6)-GlcNAcbeta1Me, was also used to map the ligand interactions at the VCNA lectin binding site.
T8 1144-1245 Sentence denotes It is well known that VCNA can hydrolyze both alpha-2,3- and alpha-2,6-linked sialic acid substrates.
T9 1246-1441 Sentence denotes In this study using alpha-2,3-sialyllactose co-crystallized with VCNA it was revealed that the inhibitor 2-deoxy-2,3-didehydro-N-acetylneuraminic acid (Neu5Ac2en) was bound at the catalytic site.
T10 1442-1569 Sentence denotes This observation supports the notion that VCNA can produce its own inhibitor and has been further confirmed by 1H NMR analysis.
T11 1570-1777 Sentence denotes The discovery of the sialic acid binding site in the N-lectin-like domain suggests that this might help target VCNA to sialic acid-rich environments, thereby enhancing the catalytic efficiency of the enzyme.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 0-11 https://glytoucan.org/Structures/Glycans/G81533KY denotes Sialic acid
T2 163-174 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T3 216-219 https://glytoucan.org/Structures/Glycans/G46613JI denotes GM1
T4 216-219 https://glytoucan.org/Structures/Glycans/G48558GR denotes GM1
T5 669-692 https://glytoucan.org/Structures/Glycans/G50850NI denotes N-acetylneuraminic acid
T6 669-692 https://glytoucan.org/Structures/Glycans/G81533KY denotes N-acetylneuraminic acid
T7 694-700 https://glytoucan.org/Structures/Glycans/G81533KY denotes Neu5Ac
T8 864-875 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T9 1222-1233 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T10 1373-1396 https://glytoucan.org/Structures/Glycans/G50850NI denotes N-acetylneuraminic acid
T11 1373-1396 https://glytoucan.org/Structures/Glycans/G81533KY denotes N-acetylneuraminic acid
T12 1591-1602 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T13 1689-1700 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid

Glycosmos6-GlycoEpitope

Id Subject Object Predicate Lexical cue
T1 216-219 http://www.glycoepitope.jp/epitopes/EP0050 denotes GM1

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 216-219 Glycan denotes GM1 https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR
T2 694-700 Glycan denotes Neu5Ac https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 27-42 Disease denotes Vibrio cholerae http://purl.obolibrary.org/obo/MONDO_0015766
T2 58-73 Disease denotes Vibrio cholerae http://purl.obolibrary.org/obo/MONDO_0015766
T3 143-150 Disease denotes cholera http://purl.obolibrary.org/obo/MONDO_0015766
T4 238-245 Disease denotes cholera http://purl.obolibrary.org/obo/MONDO_0015766

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 216-219 Glycan denotes GM1 https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR
T2 694-700 Glycan denotes Neu5Ac https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR

GlyCosmos-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 216-219 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes GM1 http://www.glycoepitope.jp/epitopes/EP0050

GlyCosmos15-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T1 27-42 Disease denotes Vibrio cholerae MONDO:0015766
T2 58-73 Disease denotes Vibrio cholerae MONDO:0015766
T3 143-150 Disease denotes cholera MONDO:0015766
T4 238-245 Disease denotes cholera MONDO:0015766

GlyCosmos15-Taxon

Id Subject Object Predicate Lexical cue db_id
T1 27-42 Organism denotes Vibrio cholerae 666
T2 58-73 Organism denotes Vibrio cholerae 666

GlyCosmos15-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 216-219 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes GM1 http://www.glycoepitope.jp/epitopes/EP0050

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-57 Sentence denotes Sialic acid recognition by Vibrio cholerae neuraminidase.
T2 58-252 Sentence denotes Vibrio cholerae neuraminidase (VCNA) plays a significant role in the pathogenesis of cholera by removing sialic acid from higher order gangliosides to unmask GM1, the receptor for cholera toxin.
T3 253-490 Sentence denotes We previously showed that the structure of VCNA is composed of a central beta-propeller catalytic domain flanked by two lectin-like domains; however the nature of the carbohydrates recognized by these lectin domains has remained unknown.
T4 491-616 Sentence denotes We present here structures of the enzyme in complex with two substrates, alpha-2,3-sialyllactose and alpha-2,6-sialyllactose.
T5 617-783 Sentence denotes Both substrate complexes reveal the alpha-anomer of N-acetylneuraminic acid (Neu5Ac) bound to the N-terminal lectin domain, thereby revealing the role of this domain.
T6 784-953 Sentence denotes The large number of interactions suggest a relatively high binding affinity for sialic acid, which was confirmed by calorimetry, which gave a Kd approximately 30 microm.
T7 954-1143 Sentence denotes Saturation transfer difference NMR using a non-hydrolyzable substrate, Neu5,9Ac2-2-S-(alpha-2,6)-GlcNAcbeta1Me, was also used to map the ligand interactions at the VCNA lectin binding site.
T8 1144-1245 Sentence denotes It is well known that VCNA can hydrolyze both alpha-2,3- and alpha-2,6-linked sialic acid substrates.
T9 1246-1441 Sentence denotes In this study using alpha-2,3-sialyllactose co-crystallized with VCNA it was revealed that the inhibitor 2-deoxy-2,3-didehydro-N-acetylneuraminic acid (Neu5Ac2en) was bound at the catalytic site.
T10 1442-1569 Sentence denotes This observation supports the notion that VCNA can produce its own inhibitor and has been further confirmed by 1H NMR analysis.
T11 1570-1777 Sentence denotes The discovery of the sialic acid binding site in the N-lectin-like domain suggests that this might help target VCNA to sialic acid-rich environments, thereby enhancing the catalytic efficiency of the enzyme.

Lectin-Jamboree-Sentence

Id Subject Object Predicate Lexical cue
T1 0-57 Sentence denotes Sialic acid recognition by Vibrio cholerae neuraminidase.
T2 58-252 Sentence denotes Vibrio cholerae neuraminidase (VCNA) plays a significant role in the pathogenesis of cholera by removing sialic acid from higher order gangliosides to unmask GM1, the receptor for cholera toxin.
T3 253-490 Sentence denotes We previously showed that the structure of VCNA is composed of a central beta-propeller catalytic domain flanked by two lectin-like domains; however the nature of the carbohydrates recognized by these lectin domains has remained unknown.
T4 491-616 Sentence denotes We present here structures of the enzyme in complex with two substrates, alpha-2,3-sialyllactose and alpha-2,6-sialyllactose.
T5 617-783 Sentence denotes Both substrate complexes reveal the alpha-anomer of N-acetylneuraminic acid (Neu5Ac) bound to the N-terminal lectin domain, thereby revealing the role of this domain.
T6 784-953 Sentence denotes The large number of interactions suggest a relatively high binding affinity for sialic acid, which was confirmed by calorimetry, which gave a Kd approximately 30 microm.
T7 954-1143 Sentence denotes Saturation transfer difference NMR using a non-hydrolyzable substrate, Neu5,9Ac2-2-S-(alpha-2,6)-GlcNAcbeta1Me, was also used to map the ligand interactions at the VCNA lectin binding site.
T8 1144-1245 Sentence denotes It is well known that VCNA can hydrolyze both alpha-2,3- and alpha-2,6-linked sialic acid substrates.
T9 1246-1441 Sentence denotes In this study using alpha-2,3-sialyllactose co-crystallized with VCNA it was revealed that the inhibitor 2-deoxy-2,3-didehydro-N-acetylneuraminic acid (Neu5Ac2en) was bound at the catalytic site.
T10 1442-1569 Sentence denotes This observation supports the notion that VCNA can produce its own inhibitor and has been further confirmed by 1H NMR analysis.
T11 1570-1777 Sentence denotes The discovery of the sialic acid binding site in the N-lectin-like domain suggests that this might help target VCNA to sialic acid-rich environments, thereby enhancing the catalytic efficiency of the enzyme.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 27-42 OrganismTaxon denotes Vibrio cholerae 666
T2 58-73 OrganismTaxon denotes Vibrio cholerae 666