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PubMed:15184369 JSONTXT

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LitCoin-entities

Id Subject Object Predicate Lexical cue db_id
795 0-4 ChemicalEntity denotes Ca2+ MESH:D002118
796 23-27 ChemicalEntity denotes Ca2+ MESH:D002118
797 38-43 GeneOrGeneProduct denotes TRPV6 NCBIGene:55503
798 116-121 GeneOrGeneProduct denotes TRPV6 NCBIGene:55503
799 133-136 CellLine denotes HEK NCBITaxon:9606
800 169-175 ChemicalEntity denotes Ca(2+) MESH:D002118
801 184-190 ChemicalEntity denotes Ca(2+) MESH:D002118
802 250-256 ChemicalEntity denotes fura-2 MESH:D016257
803 292-298 ChemicalEntity denotes Ca(2+) MESH:D002118
804 407-413 ChemicalEntity denotes Ca(2+) MESH:D002118
805 430-434 ChemicalEntity denotes EGTA MESH:D004533
806 479-482 CellLine denotes HEK NCBITaxon:9606
807 510-515 OrganismTaxon denotes human NCBITaxon:9606
808 516-521 GeneOrGeneProduct denotes TRPV6 NCBIGene:55503
809 637-641 ChemicalEntity denotes EGTA MESH:D004533
810 685-691 ChemicalEntity denotes Ca(2+) MESH:D002118
811 789-794 GeneOrGeneProduct denotes TRPV6 NCBIGene:55503
812 833-839 ChemicalEntity denotes Ca(2+) MESH:D002118
813 876-881 GeneOrGeneProduct denotes TRPV6 NCBIGene:55503
814 918-924 ChemicalEntity denotes Ca(2+) MESH:D002118
815 996-1001 GeneOrGeneProduct denotes TRPV6 NCBIGene:55503
816 1161-1165 ChemicalEntity denotes EGTA MESH:D004533
817 1171-1176 GeneOrGeneProduct denotes TRPV6 NCBIGene:55503
818 1212-1218 ChemicalEntity denotes Ca(2+) MESH:D002118
819 1334-1339 GeneOrGeneProduct denotes TRPV6 NCBIGene:55503
820 1375-1381 ChemicalEntity denotes Ca(2+) MESH:D002118
821 1448-1453 GeneOrGeneProduct denotes TRPV6 NCBIGene:55503
822 1497-1502 OrganismTaxon denotes human NCBITaxon:9606
823 1503-1508 GeneOrGeneProduct denotes TRPV6 NCBIGene:55503
824 1524-1529 OrganismTaxon denotes human NCBITaxon:9606
825 1530-1535 GeneOrGeneProduct denotes TRPV6 NCBIGene:55503
826 1536-1548 SequenceVariant denotes Delta695-725 p|DEL|695-725|
827 1598-1604 ChemicalEntity denotes Ca(2+) MESH:D002118
828 1681-1686 OrganismTaxon denotes human NCBITaxon:9606
829 1687-1692 GeneOrGeneProduct denotes TRPV6 NCBIGene:55503
830 1693-1698 SequenceVariant denotes D542A p|SUB|D|542|A
831 1821-1826 GeneOrGeneProduct denotes TRPV6 NCBIGene:55503
832 1894-1897 CellLine denotes HEK NCBITaxon:9606

LitCoin-sentences

Id Subject Object Predicate Lexical cue
T1 0-52 Sentence denotes Ca2+ dependence of the Ca2+-selective TRPV6 channel.
T2 53-224 Sentence denotes Microfluorimetry and patch-clamp experiments were performed on TRPV6-expressing HEK cells to determine whether this Ca(2+)-sensing Ca(2+) channel is constitutively active.
T3 225-435 Sentence denotes Intact cells loaded with fura-2 had an elevated intracellular free Ca(2+) concentration ([Ca(2+)](i)), which decreased to the same level such as in non-transfected cells if external Ca(2+) was chelated by EGTA.
T4 436-761 Sentence denotes Whole cell recordings from non-transfected HEK cells and cells expressing human TRPV6 revealed the presence of a basal inward current in both types of cells when the internal solution contained 0.1 mm EGTA and 100 nm [Ca(2+)](i) or if the cytosolic Ca(2+) buffering remained undisturbed in perforated patch-clamp experiments.
T5 762-925 Sentence denotes If recombinantly expressed TRPV6 forms open channels, one would expect Ca(2+)-induced current inhibition, because TRPV6 is negatively regulated by internal Ca(2+).
T6 926-1130 Sentence denotes However, dialyzing solutions with high [Ca(2+)] such as 1 microm into TRPV6-expressing cells did not block the basal inward current, which was not different from the recordings from non-transfected cells.
T7 1131-1285 Sentence denotes In contrast, dialyzing 0.5 mm EGTA into TRPV6-expressing cells readily activated Ca(2+) inward currents, which were undetectable in non-transfected cells.
T8 1286-1491 Sentence denotes Interestingly, monovalent cations permeated the TRPV6 channels under conditions where no Ca(2+) permeation was detectable, indicating that divalent cations block TRPV6 channels from the extracellular side.
T9 1492-1787 Sentence denotes Like human TRPV6, the truncated human TRPV6(Delta695-725), which lacks the C-terminal domain required for Ca(2+)-calmodulin binding, does not form constitutive active channels, whereas the human TRPV6(D542A), carrying a point mutation in the presumed pore region, does not function as a channel.
T10 1788-1904 Sentence denotes In summary, no constitutive open TRPV6 channels were detected in patch-clamp experiments from transfected HEK cells.
T11 1905-2003 Sentence denotes However, channel activity is highly regulated by intracellular and extracellular divalent cations.

LitCoin-entities-OrganismTaxon-PD

Id Subject Object Predicate Lexical cue db_id
T1 510-515 OrganismTaxon denotes human NCBItxid:9606
T2 1497-1502 OrganismTaxon denotes human NCBItxid:9606
T3 1524-1529 OrganismTaxon denotes human NCBItxid:9606
T4 1681-1686 OrganismTaxon denotes human NCBItxid:9606

LitCoin-SeqVar

Id Subject Object Predicate Lexical cue
T1 1536-1548 SequenceVariant denotes Delta695-725
T2 1693-1698 SequenceVariant denotes D542A

LitCoin-GeneOrGeneProduct-v0

Id Subject Object Predicate Lexical cue
T1 0-3 GeneOrGeneProduct denotes Ca2
T2 23-26 GeneOrGeneProduct denotes Ca2
T3 38-43 GeneOrGeneProduct denotes TRPV6
T4 74-79 GeneOrGeneProduct denotes patch
T5 80-85 GeneOrGeneProduct denotes clamp
T6 116-121 GeneOrGeneProduct denotes TRPV6
T7 137-142 GeneOrGeneProduct denotes cells
T8 146-155 GeneOrGeneProduct denotes determine
T9 176-183 GeneOrGeneProduct denotes sensing
T10 184-198 GeneOrGeneProduct denotes Ca(2+) channel
T11 217-223 GeneOrGeneProduct denotes active
T12 232-237 GeneOrGeneProduct denotes cells
T13 250-256 GeneOrGeneProduct denotes fura-2
T14 389-394 GeneOrGeneProduct denotes cells
T15 409-417 GeneOrGeneProduct denotes (2+) was
T16 430-434 GeneOrGeneProduct denotes EGTA
T17 442-446 GeneOrGeneProduct denotes cell
T18 483-488 GeneOrGeneProduct denotes cells
T19 493-498 GeneOrGeneProduct denotes cells
T20 516-521 GeneOrGeneProduct denotes TRPV6
T21 587-592 GeneOrGeneProduct denotes cells
T22 637-641 GeneOrGeneProduct denotes EGTA
T23 675-684 GeneOrGeneProduct denotes cytosolic
T24 737-742 GeneOrGeneProduct denotes patch
T25 743-748 GeneOrGeneProduct denotes clamp
T26 765-778 GeneOrGeneProduct denotes recombinantly
T27 789-794 GeneOrGeneProduct denotes TRPV6
T28 801-805 GeneOrGeneProduct denotes open
T29 840-847 GeneOrGeneProduct denotes induced
T30 876-881 GeneOrGeneProduct denotes TRPV6
T31 896-905 GeneOrGeneProduct denotes regulated
T32 960-964 GeneOrGeneProduct denotes high
T33 974-983 GeneOrGeneProduct denotes such as 1
T34 996-1001 GeneOrGeneProduct denotes TRPV6
T35 1013-1018 GeneOrGeneProduct denotes cells
T36 1027-1032 GeneOrGeneProduct denotes block
T37 1124-1129 GeneOrGeneProduct denotes cells
T38 1134-1142 GeneOrGeneProduct denotes contrast
T39 1161-1165 GeneOrGeneProduct denotes EGTA
T40 1171-1176 GeneOrGeneProduct denotes TRPV6
T41 1188-1193 GeneOrGeneProduct denotes cells
T42 1202-1211 GeneOrGeneProduct denotes activated
T43 1279-1284 GeneOrGeneProduct denotes cells
T44 1334-1339 GeneOrGeneProduct denotes TRPV6
T45 1442-1447 GeneOrGeneProduct denotes block
T46 1448-1453 GeneOrGeneProduct denotes TRPV6
T47 1472-1485 GeneOrGeneProduct denotes extracellular
T48 1486-1490 GeneOrGeneProduct denotes side
T49 1492-1496 GeneOrGeneProduct denotes Like
T50 1503-1508 GeneOrGeneProduct denotes TRPV6
T51 1514-1523 GeneOrGeneProduct denotes truncated
T52 1530-1535 GeneOrGeneProduct denotes TRPV6
T53 1557-1562 GeneOrGeneProduct denotes lacks
T54 1567-1577 GeneOrGeneProduct denotes C-terminal
T55 1605-1615 GeneOrGeneProduct denotes calmodulin
T56 1616-1623 GeneOrGeneProduct denotes binding
T57 1652-1658 GeneOrGeneProduct denotes active
T58 1687-1692 GeneOrGeneProduct denotes TRPV6
T59 1712-1717 GeneOrGeneProduct denotes point
T60 1718-1726 GeneOrGeneProduct denotes mutation
T61 1743-1747 GeneOrGeneProduct denotes pore
T62 1816-1820 GeneOrGeneProduct denotes open
T63 1821-1826 GeneOrGeneProduct denotes TRPV6
T64 1853-1858 GeneOrGeneProduct denotes patch
T65 1859-1864 GeneOrGeneProduct denotes clamp
T66 1898-1903 GeneOrGeneProduct denotes cells
T67 1922-1930 GeneOrGeneProduct denotes activity
T68 1934-1940 GeneOrGeneProduct denotes highly
T69 1941-1950 GeneOrGeneProduct denotes regulated
T70 1972-1985 GeneOrGeneProduct denotes extracellular

LitCoin-GeneOrGeneProduct-v2

Id Subject Object Predicate Lexical cue
T1 38-43 GeneOrGeneProduct denotes TRPV6
T2 74-79 GeneOrGeneProduct denotes patch
T3 80-85 GeneOrGeneProduct denotes clamp
T4 116-121 GeneOrGeneProduct denotes TRPV6
T5 184-198 GeneOrGeneProduct denotes Ca(2+) channel
T6 250-256 GeneOrGeneProduct denotes fura-2
T7 442-446 GeneOrGeneProduct denotes cell
T8 516-521 GeneOrGeneProduct denotes TRPV6
T9 675-684 GeneOrGeneProduct denotes cytosolic
T10 737-742 GeneOrGeneProduct denotes patch
T11 743-748 GeneOrGeneProduct denotes clamp
T12 789-794 GeneOrGeneProduct denotes TRPV6
T13 876-881 GeneOrGeneProduct denotes TRPV6
T14 960-964 GeneOrGeneProduct denotes high
T15 996-1001 GeneOrGeneProduct denotes TRPV6
T16 1171-1176 GeneOrGeneProduct denotes TRPV6
T17 1334-1339 GeneOrGeneProduct denotes TRPV6
T18 1448-1453 GeneOrGeneProduct denotes TRPV6
T19 1472-1485 GeneOrGeneProduct denotes extracellular
T20 1486-1490 GeneOrGeneProduct denotes side
T21 1503-1508 GeneOrGeneProduct denotes TRPV6
T22 1514-1523 GeneOrGeneProduct denotes truncated
T23 1530-1535 GeneOrGeneProduct denotes TRPV6
T24 1567-1577 GeneOrGeneProduct denotes C-terminal
T25 1605-1615 GeneOrGeneProduct denotes calmodulin
T26 1616-1623 GeneOrGeneProduct denotes binding
T27 1687-1692 GeneOrGeneProduct denotes TRPV6
T28 1821-1826 GeneOrGeneProduct denotes TRPV6
T29 1853-1858 GeneOrGeneProduct denotes patch
T30 1859-1864 GeneOrGeneProduct denotes clamp
T31 1972-1985 GeneOrGeneProduct denotes extracellular

LitCoin-GeneOrGeneProduct-v3

Id Subject Object Predicate Lexical cue
T1 38-43 GeneOrGeneProduct denotes TRPV6
T2 116-121 GeneOrGeneProduct denotes TRPV6
T3 250-256 GeneOrGeneProduct denotes fura-2
T4 516-521 GeneOrGeneProduct denotes TRPV6
T5 789-794 GeneOrGeneProduct denotes TRPV6
T6 876-881 GeneOrGeneProduct denotes TRPV6
T7 996-1001 GeneOrGeneProduct denotes TRPV6
T8 1171-1176 GeneOrGeneProduct denotes TRPV6
T9 1334-1339 GeneOrGeneProduct denotes TRPV6
T10 1448-1453 GeneOrGeneProduct denotes TRPV6
T11 1503-1508 GeneOrGeneProduct denotes TRPV6
T12 1530-1535 GeneOrGeneProduct denotes TRPV6
T13 1605-1615 GeneOrGeneProduct denotes calmodulin
T14 1687-1692 GeneOrGeneProduct denotes TRPV6
T15 1821-1826 GeneOrGeneProduct denotes TRPV6

LitCoin_Mondo_095

Id Subject Object Predicate Lexical cue mondo_id
T1 5-15 DiseaseOrPhenotypicFeature denotes dependence 0004938

LitCoin_CellLine

Id Subject Object Predicate Lexical cue cellosaurus_accession_id
T1 133-136 CellLine denotes HEK CVCL_M624
T2 479-482 CellLine denotes HEK CVCL_M624
T3 1894-1897 CellLine denotes HEK CVCL_M624

LitCoin-Chemical-MeSH-CHEBI

Id Subject Object Predicate Lexical cue ID:
T1 250-256 ChemicalEntity denotes fura-2 D016257|ChemicalEntity
T3 430-434 ChemicalEntity denotes EGTA D004533|http://purl.obolibrary.org/obo/CHEBI_30740
T5 611-619 ChemicalEntity denotes solution D012996|http://purl.obolibrary.org/obo/CHEBI_75958
T7 637-641 ChemicalEntity denotes EGTA D004533|http://purl.obolibrary.org/obo/CHEBI_30740
T9 945-954 ChemicalEntity denotes solutions D012996
T10 1161-1165 ChemicalEntity denotes EGTA D004533|http://purl.obolibrary.org/obo/CHEBI_30740
T12 1301-1319 ChemicalEntity denotes monovalent cations D002414
T13 1425-1441 ChemicalEntity denotes divalent cations D002413
T14 1986-2002 ChemicalEntity denotes divalent cations D002413

LitCoin-NCBITaxon-2

Id Subject Object Predicate Lexical cue
T1 510-515 OrganismTaxon denotes human
T2 1497-1502 OrganismTaxon denotes human
T3 1524-1529 OrganismTaxon denotes human
T4 1681-1686 OrganismTaxon denotes human

LitCoin-training-merged

Id Subject Object Predicate Lexical cue ID: cellosaurus_accession_id
T3 1894-1897 CellLine denotes HEK CVCL_M624
T2 479-482 CellLine denotes HEK CVCL_M624
T1 133-136 CellLine denotes HEK CVCL_M624
T14 1986-2002 ChemicalEntity denotes divalent cations D002413
T13 1425-1441 ChemicalEntity denotes divalent cations D002413
T12 1301-1319 ChemicalEntity denotes monovalent cations D002414
T10 1161-1165 ChemicalEntity denotes EGTA http://purl.obolibrary.org/obo/CHEBI_30740|D004533
T9 945-954 ChemicalEntity denotes solutions D012996
T7 637-641 ChemicalEntity denotes EGTA http://purl.obolibrary.org/obo/CHEBI_30740|D004533
T5 611-619 ChemicalEntity denotes solution http://purl.obolibrary.org/obo/CHEBI_75958|D012996
T64053 430-434 ChemicalEntity denotes EGTA http://purl.obolibrary.org/obo/CHEBI_30740|D004533
T818 250-256 ChemicalEntity denotes fura-2 ChemicalEntity|D016257
T15 1821-1826 GeneOrGeneProduct denotes TRPV6
T19402 1687-1692 GeneOrGeneProduct denotes TRPV6
T67995 1605-1615 GeneOrGeneProduct denotes calmodulin
T35011 1530-1535 GeneOrGeneProduct denotes TRPV6
T11 1503-1508 GeneOrGeneProduct denotes TRPV6
T24391 1448-1453 GeneOrGeneProduct denotes TRPV6
T29518 1334-1339 GeneOrGeneProduct denotes TRPV6
T8 1171-1176 GeneOrGeneProduct denotes TRPV6
T73294 996-1001 GeneOrGeneProduct denotes TRPV6
T6 876-881 GeneOrGeneProduct denotes TRPV6
T27425 789-794 GeneOrGeneProduct denotes TRPV6
T4 516-521 GeneOrGeneProduct denotes TRPV6
T73086 250-256 GeneOrGeneProduct denotes fura-2
T77774 116-121 GeneOrGeneProduct denotes TRPV6
T61007 38-43 GeneOrGeneProduct denotes TRPV6
T6326 1681-1686 OrganismTaxon denotes human
T47902 1524-1529 OrganismTaxon denotes human
T99471 1497-1502 OrganismTaxon denotes human
T22551 510-515 OrganismTaxon denotes human
T92804 1693-1698 SequenceVariant denotes D542A
T38284 1536-1548 SequenceVariant denotes Delta695-725

sentences

Id Subject Object Predicate Lexical cue
T1 0-52 Sentence denotes Ca2+ dependence of the Ca2+-selective TRPV6 channel.
T2 53-224 Sentence denotes Microfluorimetry and patch-clamp experiments were performed on TRPV6-expressing HEK cells to determine whether this Ca(2+)-sensing Ca(2+) channel is constitutively active.
T3 225-435 Sentence denotes Intact cells loaded with fura-2 had an elevated intracellular free Ca(2+) concentration ([Ca(2+)](i)), which decreased to the same level such as in non-transfected cells if external Ca(2+) was chelated by EGTA.
T4 436-761 Sentence denotes Whole cell recordings from non-transfected HEK cells and cells expressing human TRPV6 revealed the presence of a basal inward current in both types of cells when the internal solution contained 0.1 mm EGTA and 100 nm [Ca(2+)](i) or if the cytosolic Ca(2+) buffering remained undisturbed in perforated patch-clamp experiments.
T5 762-925 Sentence denotes If recombinantly expressed TRPV6 forms open channels, one would expect Ca(2+)-induced current inhibition, because TRPV6 is negatively regulated by internal Ca(2+).
T6 926-1130 Sentence denotes However, dialyzing solutions with high [Ca(2+)] such as 1 microm into TRPV6-expressing cells did not block the basal inward current, which was not different from the recordings from non-transfected cells.
T7 1131-1285 Sentence denotes In contrast, dialyzing 0.5 mm EGTA into TRPV6-expressing cells readily activated Ca(2+) inward currents, which were undetectable in non-transfected cells.
T8 1286-1491 Sentence denotes Interestingly, monovalent cations permeated the TRPV6 channels under conditions where no Ca(2+) permeation was detectable, indicating that divalent cations block TRPV6 channels from the extracellular side.
T9 1492-1787 Sentence denotes Like human TRPV6, the truncated human TRPV6(Delta695-725), which lacks the C-terminal domain required for Ca(2+)-calmodulin binding, does not form constitutive active channels, whereas the human TRPV6(D542A), carrying a point mutation in the presumed pore region, does not function as a channel.
T10 1788-1904 Sentence denotes In summary, no constitutive open TRPV6 channels were detected in patch-clamp experiments from transfected HEK cells.
T11 1905-2003 Sentence denotes However, channel activity is highly regulated by intracellular and extracellular divalent cations.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 510-515 OrganismTaxon denotes human 9606
T2 1497-1502 OrganismTaxon denotes human 9606
T3 1524-1529 OrganismTaxon denotes human 9606
T4 1681-1686 OrganismTaxon denotes human 9606

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 273-286 Body_part denotes intracellular http://purl.obolibrary.org/obo/GO_0005622
T2 1472-1485 Body_part denotes extracellular http://purl.obolibrary.org/obo/GO_0005576
T3 1743-1747 Body_part denotes pore http://purl.obolibrary.org/obo/UBERON_0008915
T4 1954-1967 Body_part denotes intracellular http://purl.obolibrary.org/obo/GO_0005622
T5 1972-1985 Body_part denotes extracellular http://purl.obolibrary.org/obo/GO_0005576

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 549-554 Cell denotes basal http://purl.obolibrary.org/obo/CL:0002324
T2 1037-1042 Cell denotes basal http://purl.obolibrary.org/obo/CL:0002324