PubMed:15157734 JSONTXT

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":569,"end":575},"obj":"Body_part"},{"id":"T2","span":{"begin":610,"end":616},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000487"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000487"}],"text":"Characterization of a homologue of the Rel/NF-kappaB transcription factor from a beetle, Allomyrina dichotoma.\nA cDNA encoding a Rel/NF-kappaB homologue was cloned from a beetle, Allomyrina dichotoma, by reverse transcriptase-polymerase chain reactions (RT-PCR) taking advantage of the conserved Rel homology domain (RHD) to synthesize primers. The Rel/NF-kappaB homologue was designated A. dichotoma (A.d.) Rel A. The amino acid sequence of the A.d. Rel A RHD was compared with those of insect RHDs. The result showed that it has 70% identity with Tribolium castaneum Dorsal, 66% with Drosophila melanogaster Dorsal, 61% with Anopheles gambiae Gambif1, and 55% with D. melanogaster Dif. A putative phosphorylation site in the RHD, RRPS, and two putative nuclear localization signals were conserved in A.d. Rel A. A recombinant fusion protein containing the A.d. Rel A RHD was confirmed to bind specifically to the NF-kappaB site of a gene encoding A.d. coleoptericin A, an antibacterial peptide from A. dichotoma. The activity of A.d. Rel A in modulating a gene construct of the A.d. coleoptericin A promoter-luciferase reporter by expressing the A.d. coleoptericin A cDNA in a Bombyx mori cell line was analyzed. The result showed that A.d. Rel A strongly activates the A.d. coleoptericin A gene construct, whereas A.d. Rel A failed to activate the gene construct containing the mutated NF-kappaB site, suggesting the importance of the interaction between the NF-kappaB site and A.d. Rel A in the signal transduction for gene expression of antibacterial peptides in A. dichotoma."}

{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":89,"end":109},"obj":"Species:273928"},{"id":"T10","span":{"begin":683,"end":686},"obj":"Gene:35045"},{"id":"T11","span":{"begin":802,"end":805},"obj":"Species:273928"},{"id":"T12","span":{"begin":858,"end":861},"obj":"Species:273928"},{"id":"T13","span":{"begin":949,"end":952},"obj":"Species:273928"},{"id":"T14","span":{"begin":954,"end":969},"obj":"Chemical:-"},{"id":"T15","span":{"begin":1001,"end":1013},"obj":"Species:273928"},{"id":"T16","span":{"begin":1031,"end":1034},"obj":"Species:273928"},{"id":"T17","span":{"begin":1080,"end":1083},"obj":"Species:273928"},{"id":"T18","span":{"begin":1085,"end":1100},"obj":"Chemical:-"},{"id":"T19","span":{"begin":1148,"end":1151},"obj":"Species:273928"},{"id":"T2","span":{"begin":179,"end":199},"obj":"Species:273928"},{"id":"T20","span":{"begin":1153,"end":1168},"obj":"Chemical:-"},{"id":"T21","span":{"begin":1179,"end":1190},"obj":"Species:7091"},{"id":"T22","span":{"begin":1238,"end":1241},"obj":"Species:273928"},{"id":"T23","span":{"begin":1272,"end":1275},"obj":"Species:273928"},{"id":"T24","span":{"begin":1317,"end":1320},"obj":"Species:273928"},{"id":"T25","span":{"begin":1481,"end":1484},"obj":"Species:273928"},{"id":"T26","span":{"begin":1568,"end":1580},"obj":"Species:273928"},{"id":"T3","span":{"begin":388,"end":400},"obj":"Species:273928"},{"id":"T4","span":{"begin":402,"end":405},"obj":"Species:273928"},{"id":"T5","span":{"begin":446,"end":449},"obj":"Species:273928"},{"id":"T6","span":{"begin":549,"end":568},"obj":"Species:7070"},{"id":"T7","span":{"begin":586,"end":616},"obj":"Disease:MESH:C538109"},{"id":"T8","span":{"begin":627,"end":644},"obj":"Species:7165"},{"id":"T9","span":{"begin":667,"end":682},"obj":"Species:7227"}],"text":"Characterization of a homologue of the Rel/NF-kappaB transcription factor from a beetle, Allomyrina dichotoma.\nA cDNA encoding a Rel/NF-kappaB homologue was cloned from a beetle, Allomyrina dichotoma, by reverse transcriptase-polymerase chain reactions (RT-PCR) taking advantage of the conserved Rel homology domain (RHD) to synthesize primers. The Rel/NF-kappaB homologue was designated A. dichotoma (A.d.) Rel A. The amino acid sequence of the A.d. Rel A RHD was compared with those of insect RHDs. The result showed that it has 70% identity with Tribolium castaneum Dorsal, 66% with Drosophila melanogaster Dorsal, 61% with Anopheles gambiae Gambif1, and 55% with D. melanogaster Dif. A putative phosphorylation site in the RHD, RRPS, and two putative nuclear localization signals were conserved in A.d. Rel A. A recombinant fusion protein containing the A.d. Rel A RHD was confirmed to bind specifically to the NF-kappaB site of a gene encoding A.d. coleoptericin A, an antibacterial peptide from A. dichotoma. The activity of A.d. Rel A in modulating a gene construct of the A.d. coleoptericin A promoter-luciferase reporter by expressing the A.d. coleoptericin A cDNA in a Bombyx mori cell line was analyzed. The result showed that A.d. Rel A strongly activates the A.d. coleoptericin A gene construct, whereas A.d. Rel A failed to activate the gene construct containing the mutated NF-kappaB site, suggesting the importance of the interaction between the NF-kappaB site and A.d. Rel A in the signal transduction for gene expression of antibacterial peptides in A. dichotoma."}

{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":89,"end":109},"obj":"Species:273928"},{"id":"T2","span":{"begin":179,"end":199},"obj":"Species:273928"},{"id":"T3","span":{"begin":388,"end":400},"obj":"Species:273928"},{"id":"T4","span":{"begin":402,"end":405},"obj":"Species:273928"},{"id":"T5","span":{"begin":446,"end":449},"obj":"Species:273928"},{"id":"T6","span":{"begin":549,"end":568},"obj":"Species:7070"},{"id":"T7","span":{"begin":586,"end":616},"obj":"Disease:MESH:C538109"},{"id":"T8","span":{"begin":627,"end":644},"obj":"Species:7165"},{"id":"T9","span":{"begin":667,"end":682},"obj":"Species:7227"},{"id":"T10","span":{"begin":683,"end":686},"obj":"Gene:35045"},{"id":"T11","span":{"begin":802,"end":805},"obj":"Species:273928"},{"id":"T12","span":{"begin":858,"end":861},"obj":"Species:273928"},{"id":"T13","span":{"begin":949,"end":952},"obj":"Species:273928"},{"id":"T14","span":{"begin":954,"end":969},"obj":"Chemical:-"},{"id":"T15","span":{"begin":1001,"end":1013},"obj":"Species:273928"},{"id":"T16","span":{"begin":1031,"end":1034},"obj":"Species:273928"},{"id":"T17","span":{"begin":1080,"end":1083},"obj":"Species:273928"},{"id":"T18","span":{"begin":1085,"end":1100},"obj":"Chemical:-"},{"id":"T19","span":{"begin":1148,"end":1151},"obj":"Species:273928"},{"id":"T20","span":{"begin":1153,"end":1168},"obj":"Chemical:-"},{"id":"T21","span":{"begin":1179,"end":1190},"obj":"Species:7091"},{"id":"T22","span":{"begin":1238,"end":1241},"obj":"Species:273928"},{"id":"T23","span":{"begin":1272,"end":1275},"obj":"Species:273928"},{"id":"T24","span":{"begin":1317,"end":1320},"obj":"Species:273928"},{"id":"T25","span":{"begin":1481,"end":1484},"obj":"Species:273928"},{"id":"T26","span":{"begin":1568,"end":1580},"obj":"Species:273928"}],"text":"Characterization of a homologue of the Rel/NF-kappaB transcription factor from a beetle, Allomyrina dichotoma.\nA cDNA encoding a Rel/NF-kappaB homologue was cloned from a beetle, Allomyrina dichotoma, by reverse transcriptase-polymerase chain reactions (RT-PCR) taking advantage of the conserved Rel homology domain (RHD) to synthesize primers. The Rel/NF-kappaB homologue was designated A. dichotoma (A.d.) Rel A. The amino acid sequence of the A.d. Rel A RHD was compared with those of insect RHDs. The result showed that it has 70% identity with Tribolium castaneum Dorsal, 66% with Drosophila melanogaster Dorsal, 61% with Anopheles gambiae Gambif1, and 55% with D. melanogaster Dif. A putative phosphorylation site in the RHD, RRPS, and two putative nuclear localization signals were conserved in A.d. Rel A. A recombinant fusion protein containing the A.d. Rel A RHD was confirmed to bind specifically to the NF-kappaB site of a gene encoding A.d. coleoptericin A, an antibacterial peptide from A. dichotoma. The activity of A.d. Rel A in modulating a gene construct of the A.d. coleoptericin A promoter-luciferase reporter by expressing the A.d. coleoptericin A cDNA in a Bombyx mori cell line was analyzed. The result showed that A.d. Rel A strongly activates the A.d. coleoptericin A gene construct, whereas A.d. Rel A failed to activate the gene construct containing the mutated NF-kappaB site, suggesting the importance of the interaction between the NF-kappaB site and A.d. Rel A in the signal transduction for gene expression of antibacterial peptides in A. dichotoma."}

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":317,"end":320},"obj":"Disease"},{"id":"T2","span":{"begin":457,"end":460},"obj":"Disease"},{"id":"T3","span":{"begin":727,"end":730},"obj":"Disease"},{"id":"T4","span":{"begin":755,"end":783},"obj":"Disease"},{"id":"T5","span":{"begin":869,"end":872},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0006955"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0006955"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0006955"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MONDO_0000179"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MONDO_0006955"}],"text":"Characterization of a homologue of the Rel/NF-kappaB transcription factor from a beetle, Allomyrina dichotoma.\nA cDNA encoding a Rel/NF-kappaB homologue was cloned from a beetle, Allomyrina dichotoma, by reverse transcriptase-polymerase chain reactions (RT-PCR) taking advantage of the conserved Rel homology domain (RHD) to synthesize primers. The Rel/NF-kappaB homologue was designated A. dichotoma (A.d.) Rel A. The amino acid sequence of the A.d. Rel A RHD was compared with those of insect RHDs. The result showed that it has 70% identity with Tribolium castaneum Dorsal, 66% with Drosophila melanogaster Dorsal, 61% with Anopheles gambiae Gambif1, and 55% with D. melanogaster Dif. A putative phosphorylation site in the RHD, RRPS, and two putative nuclear localization signals were conserved in A.d. Rel A. A recombinant fusion protein containing the A.d. Rel A RHD was confirmed to bind specifically to the NF-kappaB site of a gene encoding A.d. coleoptericin A, an antibacterial peptide from A. dichotoma. The activity of A.d. Rel A in modulating a gene construct of the A.d. coleoptericin A promoter-luciferase reporter by expressing the A.d. coleoptericin A cDNA in a Bombyx mori cell line was analyzed. The result showed that A.d. Rel A strongly activates the A.d. coleoptericin A gene construct, whereas A.d. Rel A failed to activate the gene construct containing the mutated NF-kappaB site, suggesting the importance of the interaction between the NF-kappaB site and A.d. Rel A in the signal transduction for gene expression of antibacterial peptides in A. dichotoma."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":89,"end":109},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":179,"end":199},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":549,"end":568},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":586,"end":609},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":627,"end":644},"obj":"OrganismTaxon"},{"id":"T6","span":{"begin":670,"end":682},"obj":"OrganismTaxon"},{"id":"T8","span":{"begin":1179,"end":1190},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"273928"},{"id":"A2","pred":"db_id","subj":"T2","obj":"273928"},{"id":"A3","pred":"db_id","subj":"T3","obj":"7070"},{"id":"A4","pred":"db_id","subj":"T4","obj":"7227"},{"id":"A5","pred":"db_id","subj":"T5","obj":"7165"},{"id":"A6","pred":"db_id","subj":"T6","obj":"2771363"},{"id":"A7","pred":"db_id","subj":"T6","obj":"80614"},{"id":"A8","pred":"db_id","subj":"T8","obj":"7091"}],"text":"Characterization of a homologue of the Rel/NF-kappaB transcription factor from a beetle, Allomyrina dichotoma.\nA cDNA encoding a Rel/NF-kappaB homologue was cloned from a beetle, Allomyrina dichotoma, by reverse transcriptase-polymerase chain reactions (RT-PCR) taking advantage of the conserved Rel homology domain (RHD) to synthesize primers. The Rel/NF-kappaB homologue was designated A. dichotoma (A.d.) Rel A. The amino acid sequence of the A.d. Rel A RHD was compared with those of insect RHDs. The result showed that it has 70% identity with Tribolium castaneum Dorsal, 66% with Drosophila melanogaster Dorsal, 61% with Anopheles gambiae Gambif1, and 55% with D. melanogaster Dif. A putative phosphorylation site in the RHD, RRPS, and two putative nuclear localization signals were conserved in A.d. Rel A. A recombinant fusion protein containing the A.d. Rel A RHD was confirmed to bind specifically to the NF-kappaB site of a gene encoding A.d. coleoptericin A, an antibacterial peptide from A. dichotoma. The activity of A.d. Rel A in modulating a gene construct of the A.d. coleoptericin A promoter-luciferase reporter by expressing the A.d. coleoptericin A cDNA in a Bombyx mori cell line was analyzed. The result showed that A.d. Rel A strongly activates the A.d. coleoptericin A gene construct, whereas A.d. Rel A failed to activate the gene construct containing the mutated NF-kappaB site, suggesting the importance of the interaction between the NF-kappaB site and A.d. Rel A in the signal transduction for gene expression of antibacterial peptides in A. dichotoma."}