PubMed:15134654
Annnotations
{"target":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/15134654","sourcedb":"PubMed","sourceid":"15134654","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/15134654","text":"Fluorescence analysis of hormone binding activities of wheat germ agglutinin.\nWheat germ agglutinin (WGA) from embryos of the monocotyledonous plant Triticum vulgaris (Graminaceae) is a carbohydrate binding protein characterized by high specificity to N-acetyl-d-glucosamine and N-acetyl-d-neuraminic acid. In this study we show that parallel to its carbohydrate binding activities, WGA binds with several orders of magnitude higher affinity adenine, adenine-related cytokinins: kinetin, zeatin and isopentenyl-adenine as well as abscisic and gibberellic acids (K(d) 0.43-0.65 microM). Its interactions with these ligands cause conformational rearrangements in the protein molecules and significant enhancement of the protein tryptophan fluorescence (up to 60%) allowing characterization of the protein-hormone complexes. Dimeric WGA molecules possess two different classes of binding sites for the fluorescent hydrophobic probe 2-(p-toluidinyl) naphthalene sulfonic acid (TNS) as suggested by the sigmoid shape of the fluorescence titration curve and the value of the Hill coefficient (n(H) 1.6+/-0.3). The plant hormones displace part of the bound TNS probe and share the higher affinity TNS binding sites. These results characterize WGA as a hormone-binding protein.","tracks":[{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":973,"end":976},"obj":"Disease"},{"id":"T2","span":{"begin":1150,"end":1153},"obj":"Disease"},{"id":"T3","span":{"begin":1190,"end":1193},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0008582"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0008582"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0008582"},{"subj":"T1","pred":"source","obj":"mondo_disease"},{"subj":"T2","pred":"source","obj":"mondo_disease"},{"subj":"T3","pred":"source","obj":"mondo_disease"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":55,"end":60},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":78,"end":83},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":149,"end":157},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"4565"},{"id":"A2","pred":"db_id","subj":"T2","obj":"4565"},{"id":"A3","pred":"db_id","subj":"T3","obj":"4564"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"},{"subj":"T3","pred":"source","obj":"NCBITAXON"}]},{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":111,"end":118},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000226"},{"subj":"T1","pred":"source","obj":"Anatomy-MAT"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"mondo_disease","color":"#98ec93","default":true},{"id":"NCBITAXON","color":"#ec93b2"},{"id":"Anatomy-MAT","color":"#93ccec"}]}]}}