PubMed:15126743 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/15126743","sourcedb":"PubMed","sourceid":"15126743","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/15126743","text":"Epstein-Barr virus (EBV) latent membrane protein 1 induces interleukin-8 through the nuclear factor-kappa B signaling pathway in EBV-infected nasopharyngeal carcinoma cell line.\nBACKGROUND/OBJECTIVES: Nasopharyngeal carcinoma (NPC) is a highly invasive and metastatic malignant tumor and is associated with Epstein-Barr virus (EBV) infection that exhibits type II latency. Angiogenesis is essential for tumor growth, invasion, and metastasis. Our previous studies have indicated that interleukin (IL)-8 was over-expressed in many NPC tissues and was found to be significantly correlated with angiogenesis by immunohistochemistry.\nSTUDY DESIGN: In vitro design.\nMETHODS: The influence of the EBV genome for IL-8 gene expression was studied using the EBV-genome-positive and -negative epithelial/NPC hybrid cell line NPC-KT. The EBV-positive and -negative clones were selected by polymerase chain reaction and in situ hybridization.\nRESULTS: EBV-positive clones expressed abundant IL-8 mRNA compared with EBV-negative clones. This result indicated that over-expression of IL-8 depended on the presence of EBV genomes in NPC-KT cells. Two encoded genes, latent membrane protein (LMP)1 and EBV-encoded small RNAs (EBERs), expressed in NPC were transfected in EBV-negative NPC-KT cells. LMP1 transactivated the IL-8 promoter, whereas EBERs did not. Moreover, the nuclear factor (NF)-kappa B binding site in the IL-8 promoter was essential for the response to LMP1, and the activator protein (AP)-1 binding site played only a partial role.\nCONCLUSIONS: LMP1 induces IL-8 mainly through the activation of NF-kappa B and partly through AP-1 in NPC model cell lines, NPC-KT, and this suggests that LMP1 plays an important role in the angiogenesis of NPC.","tracks":[{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":706,"end":710},"obj":"gene:3576"},{"id":"T1","span":{"begin":794,"end":797},"obj":"disease:C2931822"},{"id":"T2","span":{"begin":706,"end":710},"obj":"gene:3576"},{"id":"T3","span":{"begin":815,"end":818},"obj":"disease:C2931822"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"attributes":[{"subj":"T0","pred":"source","obj":"DisGeNET"},{"subj":"T1","pred":"source","obj":"DisGeNET"},{"subj":"T2","pred":"source","obj":"DisGeNET"},{"subj":"T3","pred":"source","obj":"DisGeNET"}]},{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T1","span":{"begin":201,"end":629},"obj":"OBJECTIVE"},{"id":"T2","span":{"begin":644,"end":660},"obj":"METHODS"},{"id":"T3","span":{"begin":670,"end":930},"obj":"METHODS"},{"id":"T4","span":{"begin":940,"end":1533},"obj":"RESULTS"},{"id":"T5","span":{"begin":1547,"end":1745},"obj":"CONCLUSIONS"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubMed_Structured_Abstracts"},{"subj":"T2","pred":"source","obj":"PubMed_Structured_Abstracts"},{"subj":"T3","pred":"source","obj":"PubMed_Structured_Abstracts"},{"subj":"T4","pred":"source","obj":"PubMed_Structured_Abstracts"},{"subj":"T5","pred":"source","obj":"PubMed_Structured_Abstracts"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"DisGeNET","color":"#b7ec93","default":true},{"id":"PubMed_Structured_Abstracts","color":"#ec93d1"}]}]}}