PubMed:1510878 JSON TXT

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jnlpba-st-training

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":41,"end":46},"obj":"protein"},{"id":"T2","span":{"begin":51,"end":56},"obj":"protein"},{"id":"T3","span":{"begin":173,"end":178},"obj":"protein"},{"id":"T4","span":{"begin":183,"end":188},"obj":"protein"},{"id":"T5","span":{"begin":299,"end":304},"obj":"protein"},{"id":"T6","span":{"begin":309,"end":314},"obj":"protein"},{"id":"T7","span":{"begin":553,"end":558},"obj":"protein"},{"id":"T8","span":{"begin":563,"end":568},"obj":"protein"},{"id":"T9","span":{"begin":674,"end":679},"obj":"protein"},{"id":"T10","span":{"begin":796,"end":801},"obj":"protein"},{"id":"T11","span":{"begin":806,"end":811},"obj":"protein"},{"id":"T12","span":{"begin":1186,"end":1198},"obj":"cell_line"},{"id":"T13","span":{"begin":1400,"end":1405},"obj":"protein"},{"id":"T14","span":{"begin":1410,"end":1415},"obj":"protein"},{"id":"T15","span":{"begin":1475,"end":1487},"obj":"cell_line"}],"text":"Mitogen stimulation of T-cells increases c-Fos and c-Jun protein levels, AP-1 binding and AP-1 transcriptional activity.\nWe have analysed the effect of mitogenic lectins on c-Fos and c-Jun protein levels as well as on activator protein-1 (AP-1) binding and enhancer activity in Jurkat T-cells. Both c-Fos and c-Jun protein levels were increased after Con A and PHA stimulation. Since T-cell stimulation increases both intracellular Ca2+ and cAMP levels and activates protein kinase C (PKC), the possible involvement of these intracellular messengers in c-Fos and c-Jun induction was tested. PMA, which directly activates PKC, mimicked the effect of the lectins on c-Fos and c-Jun, but elevation of either intracellular Ca2+ or cAMP levels had little or no effect. The mitogen-induced increase of c-Fos and c-Jun immunoreactivity was inhibited by H-7, a kinase inhibitor with relatively high specificity for PKC, and less efficiently by H-8, a structurally related kinase inhibitor less active on PKC, but more active on cyclic nucleotide-dependent kinases. Con A stimulation was found to increase both binding of AP-1 to the AP-1 consensus sequence, TRE, and AP-1 enhancer activity, in Jurkat cells. PMA was also found to increase the AP-1 enhancer activity, whereas elevation of Ca2+ or cAMP had only minor effects. We conclude that stimulation with mitogenic lectins is sufficient to increase both c-Fos and c-Jun protein levels, AP-1 binding and AP-1 enhancer activity in Jurkat cells and that they act via mechanisms that could involve the activation of PKC."}

genia-medco-coref

semrep-sample

pubmed-sentences-benchmark

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":120},"obj":"Sentence"},{"id":"S2","span":{"begin":121,"end":293},"obj":"Sentence"},{"id":"S3","span":{"begin":294,"end":377},"obj":"Sentence"},{"id":"S4","span":{"begin":378,"end":590},"obj":"Sentence"},{"id":"S5","span":{"begin":591,"end":763},"obj":"Sentence"},{"id":"S6","span":{"begin":764,"end":1056},"obj":"Sentence"},{"id":"S7","span":{"begin":1057,"end":1199},"obj":"Sentence"},{"id":"S8","span":{"begin":1200,"end":1316},"obj":"Sentence"},{"id":"S9","span":{"begin":1317,"end":1562},"obj":"Sentence"}],"text":"Mitogen stimulation of T-cells increases c-Fos and c-Jun protein levels, AP-1 binding and AP-1 transcriptional activity.\nWe have analysed the effect of mitogenic lectins on c-Fos and c-Jun protein levels as well as on activator protein-1 (AP-1) binding and enhancer activity in Jurkat T-cells. Both c-Fos and c-Jun protein levels were increased after Con A and PHA stimulation. Since T-cell stimulation increases both intracellular Ca2+ and cAMP levels and activates protein kinase C (PKC), the possible involvement of these intracellular messengers in c-Fos and c-Jun induction was tested. PMA, which directly activates PKC, mimicked the effect of the lectins on c-Fos and c-Jun, but elevation of either intracellular Ca2+ or cAMP levels had little or no effect. The mitogen-induced increase of c-Fos and c-Jun immunoreactivity was inhibited by H-7, a kinase inhibitor with relatively high specificity for PKC, and less efficiently by H-8, a structurally related kinase inhibitor less active on PKC, but more active on cyclic nucleotide-dependent kinases. Con A stimulation was found to increase both binding of AP-1 to the AP-1 consensus sequence, TRE, and AP-1 enhancer activity, in Jurkat cells. PMA was also found to increase the AP-1 enhancer activity, whereas elevation of Ca2+ or cAMP had only minor effects. We conclude that stimulation with mitogenic lectins is sufficient to increase both c-Fos and c-Jun protein levels, AP-1 binding and AP-1 enhancer activity in Jurkat cells and that they act via mechanisms that could involve the activation of PKC."}

GENIAcorpus

{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":41,"end":46},"obj":"protein_molecule"},{"id":"T2","span":{"begin":51,"end":56},"obj":"protein_molecule"},{"id":"T3","span":{"begin":95,"end":119},"obj":"other_name"},{"id":"T4","span":{"begin":173,"end":178},"obj":"protein_molecule"},{"id":"T5","span":{"begin":183,"end":188},"obj":"protein_molecule"},{"id":"T6","span":{"begin":299,"end":304},"obj":"protein_molecule"},{"id":"T7","span":{"begin":309,"end":314},"obj":"protein_molecule"},{"id":"T8","span":{"begin":553,"end":558},"obj":"protein_molecule"},{"id":"T9","span":{"begin":563,"end":568},"obj":"protein_molecule"},{"id":"T10","span":{"begin":591,"end":594},"obj":"other_organic_compound"},{"id":"T11","span":{"begin":674,"end":679},"obj":"protein_molecule"},{"id":"T12","span":{"begin":796,"end":801},"obj":"protein_molecule"},{"id":"T13","span":{"begin":806,"end":811},"obj":"protein_molecule"},{"id":"T14","span":{"begin":1186,"end":1198},"obj":"cell_line"},{"id":"T15","span":{"begin":1200,"end":1203},"obj":"other_organic_compound"},{"id":"T16","span":{"begin":1400,"end":1405},"obj":"protein_molecule"},{"id":"T17","span":{"begin":1410,"end":1415},"obj":"protein_molecule"},{"id":"T18","span":{"begin":1475,"end":1487},"obj":"cell_line"}],"text":"Mitogen stimulation of T-cells increases c-Fos and c-Jun protein levels, AP-1 binding and AP-1 transcriptional activity.\nWe have analysed the effect of mitogenic lectins on c-Fos and c-Jun protein levels as well as on activator protein-1 (AP-1) binding and enhancer activity in Jurkat T-cells. Both c-Fos and c-Jun protein levels were increased after Con A and PHA stimulation. Since T-cell stimulation increases both intracellular Ca2+ and cAMP levels and activates protein kinase C (PKC), the possible involvement of these intracellular messengers in c-Fos and c-Jun induction was tested. PMA, which directly activates PKC, mimicked the effect of the lectins on c-Fos and c-Jun, but elevation of either intracellular Ca2+ or cAMP levels had little or no effect. The mitogen-induced increase of c-Fos and c-Jun immunoreactivity was inhibited by H-7, a kinase inhibitor with relatively high specificity for PKC, and less efficiently by H-8, a structurally related kinase inhibitor less active on PKC, but more active on cyclic nucleotide-dependent kinases. Con A stimulation was found to increase both binding of AP-1 to the AP-1 consensus sequence, TRE, and AP-1 enhancer activity, in Jurkat cells. PMA was also found to increase the AP-1 enhancer activity, whereas elevation of Ca2+ or cAMP had only minor effects. We conclude that stimulation with mitogenic lectins is sufficient to increase both c-Fos and c-Jun protein levels, AP-1 binding and AP-1 enhancer activity in Jurkat cells and that they act via mechanisms that could involve the activation of PKC."}

metamap-sample