| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-78 |
Sentence |
denotes |
High-affinity binders selected from designed ankyrin repeat protein libraries. |
| T2 |
79-192 |
Sentence |
denotes |
We report here the evolution of ankyrin repeat (AR) proteins in vitro for specific, high-affinity target binding. |
| T3 |
193-340 |
Sentence |
denotes |
Using a consensus design strategy, we generated combinatorial libraries of AR proteins of varying repeat numbers with diversified binding surfaces. |
| T4 |
341-513 |
Sentence |
denotes |
Libraries of two and three repeats, flanked by 'capping repeats,' were used in ribosome-display selections against maltose binding protein (MBP) and two eukaryotic kinases. |
| T5 |
514-713 |
Sentence |
denotes |
We rapidly enriched target-specific binders with affinities in the low nanomolar range and determined the crystal structure of one of the selected AR proteins in complex with MBP at 2.3 A resolution. |
| T6 |
714-865 |
Sentence |
denotes |
The interaction relies on the randomized positions of the designed AR protein and is comparable to natural, heterodimeric protein-protein interactions. |
| T7 |
866-1075 |
Sentence |
denotes |
Thus, our AR protein libraries are valuable sources for binding molecules and, because of the very favorable biophysical properties of the designed AR proteins, an attractive alternative to antibody libraries. |
