PubMed:15047715
Annnotations
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-88 | Sentence | denotes | Wwp2, an E3 ubiquitin ligase that targets transcription factor Oct-4 for ubiquitination. |
| T2 | 89-201 | Sentence | denotes | The POU transcription factor Oct-4 is a master regulator affecting the fate of pluripotent embryonic stem cells. |
| T3 | 202-326 | Sentence | denotes | However, the precise mechanisms by which the activation and expression of Oct-4 are regulated still remain to be elucidated. |
| T4 | 327-480 | Sentence | denotes | We describe here a novel murine ubiquitin ligase, Wwp2, that specifically interacts with Oct-4 and promotes its ubiquitination both in vivo and in vitro. |
| T5 | 481-588 | Sentence | denotes | Remarkably, the expression of a catalytically inactive point mutant of Wwp2 abolishes Oct-4 ubiquitination. |
| T6 | 589-674 | Sentence | denotes | Moreover, Wwp2 promotes Oct-4 degradation in the presence of overexpressed ubiquitin. |
| T7 | 675-741 | Sentence | denotes | The degradation is blocked by treatment with proteasome inhibitor. |
| T8 | 742-868 | Sentence | denotes | Fusion of a single ubiquitin to Oct-4 inactivates its transcriptional activity in a heterologous Oct-4-driven reporter system. |
| T9 | 869-988 | Sentence | denotes | Furthermore, overexpression of Wwp2 in embryonic stem cells significantly reduces the Oct-4-transcriptional activities. |
| T10 | 989-1183 | Sentence | denotes | Collectively, we demonstrate for the first time that Oct-4 can be post-translationally modified by ubiquitination and that this modification dramatically suppresses its transcriptional activity. |
| T11 | 1184-1420 | Sentence | denotes | These results reveal that the functional status of Oct-4, in addition to its expression level, dictates its transcriptional activity, and the results open up a new avenue to understand how Oct-4 defines the fate of embryonic stem cells. |
Glycosmos6-MAT
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 190-200 | http://purl.obolibrary.org/obo/MAT_0000185 | denotes | stem cells |
| T2 | 190-194 | http://purl.obolibrary.org/obo/MAT_0000239 | denotes | stem |
| T3 | 918-928 | http://purl.obolibrary.org/obo/MAT_0000185 | denotes | stem cells |
| T4 | 918-922 | http://purl.obolibrary.org/obo/MAT_0000239 | denotes | stem |
| T5 | 1409-1419 | http://purl.obolibrary.org/obo/MAT_0000185 | denotes | stem cells |
| T6 | 1409-1413 | http://purl.obolibrary.org/obo/MAT_0000239 | denotes | stem |
2015-BEL-Sample
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 327-479 | complex(p(MGI:Wwp2),p(MGI:Pou5f1)) directlyIncreases deg(p(MGI:Pou5f1)) | denotes | We describe here a novel murine ubiquitin ligase, Wwp2, that specifically interacts with Oct-4 and promotes its ubiquitination both in vivo and in vitro |
2015-BEL-Sample-2
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| BEL:20000540 | 327-479 | complex(p(MGI:Wwp2),p(MGI:Pou5f1)) directlyIncreases deg(p(MGI:Pou5f1)) | denotes | We describe here a novel murine ubiquitin ligase, Wwp2, that specifically interacts with Oct-4 and promotes its ubiquitination both in vivo and in vitro |
| BEL:20033314 | 327-479 | cat(p(MGI:Wwp2)) directlyIncreases deg(p(MGI:Pou5f1)) | denotes | We describe here a novel murine ubiquitin ligase, Wwp2, that specifically interacts with Oct-4 and promotes its ubiquitination both in vivo and in vitro |
| BEL:20035050 | 327-479 | complex(p(MGI:Wwp2),p(MGI:Pou5f1)) directlyIncreases deg(p(MGI:Pou5f1)) | denotes | We describe here a novel murine ubiquitin ligase, Wwp2, that specifically interacts with Oct-4 and promotes its ubiquitination both in vivo and in vitro |
Anatomy-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 190-200 | Body_part | denotes | stem cells | http://purl.obolibrary.org/obo/MAT_0000185 |
| T2 | 918-928 | Body_part | denotes | stem cells | http://purl.obolibrary.org/obo/MAT_0000185 |
| T3 | 1409-1419 | Body_part | denotes | stem cells | http://purl.obolibrary.org/obo/MAT_0000185 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 190-200 | Body_part | denotes | stem cells | http://purl.obolibrary.org/obo/CL_0000034 |
| T2 | 918-928 | Body_part | denotes | stem cells | http://purl.obolibrary.org/obo/CL_0000034 |
| T3 | 1409-1419 | Body_part | denotes | stem cells | http://purl.obolibrary.org/obo/CL_0000034 |
CL-cell
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 180-200 | Cell | denotes | embryonic stem cells | http://purl.obolibrary.org/obo/CL:0002322 |
| T2 | 908-928 | Cell | denotes | embryonic stem cells | http://purl.obolibrary.org/obo/CL:0002322 |
| T3 | 1399-1419 | Cell | denotes | embryonic stem cells | http://purl.obolibrary.org/obo/CL:0002322 |