| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-222 |
Sentence |
denotes |
Silencing expression of the clusterin/apolipoprotein j gene in human cancer cells using small interfering RNA induces spontaneous apoptosis, reduced growth ability, and cell sensitization to genotoxic and oxidative stress. |
| TextSentencer_T2 |
223-506 |
Sentence |
denotes |
Clusterin/Apolipoprotein J (CLU) is a heterodimeric ubiquitously expressed secreted glycoprotein that is implicated in several physiological processes and is differentially expressed in many severe physiological disturbances, including tumor formation and in vivo cancer progression. |
| TextSentencer_T3 |
507-647 |
Sentence |
denotes |
Despite extensive efforts, clarification of CLU's biological role has been exceptionally difficult and its precise function remains elusive. |
| TextSentencer_T4 |
648-791 |
Sentence |
denotes |
Short RNA duplexes, referred to as small interfering RNAs (siRNAs), provide a new approach for the elucidation of gene function in human cells. |
| TextSentencer_T5 |
792-963 |
Sentence |
denotes |
Here, we describe siRNA-mediated CLU gene silencing in osteosarcoma and prostate human cancer cells and illustrate that CLU mRNA is amenable to siRNA-mediated degradation. |
| TextSentencer_T6 |
964-1128 |
Sentence |
denotes |
Our data demonstrate that CLU knockdown in human cancer cells induces significant reduction of cellular growth and higher rates of spontaneous endogenous apoptosis. |
| TextSentencer_T7 |
1129-1296 |
Sentence |
denotes |
Moreover, CLU knockdown cancer cells were significantly sensitized to both genotoxic and oxidative stress induced by chemotherapeutic drugs and H(2)O(2), respectively. |
| TextSentencer_T8 |
1297-1484 |
Sentence |
denotes |
These effects were more pronounced in cell lines that express high endogenous steady-state levels of the CLU protein and occur through hyperactivation of the cellular apoptotic machinery. |
| TextSentencer_T9 |
1485-1692 |
Sentence |
denotes |
Overall, our results reveal that, in the distinct cellular contexts of the osteosarcoma and prostate cancer cells assayed, CLU is a central molecule in cell homeostasis that exerts a cytoprotective function. |
| TextSentencer_T10 |
1693-1911 |
Sentence |
denotes |
The described CLU-specific siRNA oligonucleotides that can potently silence CLU gene expression may thus prove valuable agents during antitumor therapy or at other pathological conditions where CLU has been implicated. |
| T1 |
0-222 |
Sentence |
denotes |
Silencing expression of the clusterin/apolipoprotein j gene in human cancer cells using small interfering RNA induces spontaneous apoptosis, reduced growth ability, and cell sensitization to genotoxic and oxidative stress. |
| T2 |
223-506 |
Sentence |
denotes |
Clusterin/Apolipoprotein J (CLU) is a heterodimeric ubiquitously expressed secreted glycoprotein that is implicated in several physiological processes and is differentially expressed in many severe physiological disturbances, including tumor formation and in vivo cancer progression. |
| T3 |
507-647 |
Sentence |
denotes |
Despite extensive efforts, clarification of CLU's biological role has been exceptionally difficult and its precise function remains elusive. |
| T4 |
648-791 |
Sentence |
denotes |
Short RNA duplexes, referred to as small interfering RNAs (siRNAs), provide a new approach for the elucidation of gene function in human cells. |
| T5 |
792-963 |
Sentence |
denotes |
Here, we describe siRNA-mediated CLU gene silencing in osteosarcoma and prostate human cancer cells and illustrate that CLU mRNA is amenable to siRNA-mediated degradation. |
| T6 |
964-1128 |
Sentence |
denotes |
Our data demonstrate that CLU knockdown in human cancer cells induces significant reduction of cellular growth and higher rates of spontaneous endogenous apoptosis. |
| T7 |
1129-1296 |
Sentence |
denotes |
Moreover, CLU knockdown cancer cells were significantly sensitized to both genotoxic and oxidative stress induced by chemotherapeutic drugs and H(2)O(2), respectively. |
| T8 |
1297-1484 |
Sentence |
denotes |
These effects were more pronounced in cell lines that express high endogenous steady-state levels of the CLU protein and occur through hyperactivation of the cellular apoptotic machinery. |
| T9 |
1485-1692 |
Sentence |
denotes |
Overall, our results reveal that, in the distinct cellular contexts of the osteosarcoma and prostate cancer cells assayed, CLU is a central molecule in cell homeostasis that exerts a cytoprotective function. |
| T10 |
1693-1911 |
Sentence |
denotes |
The described CLU-specific siRNA oligonucleotides that can potently silence CLU gene expression may thus prove valuable agents during antitumor therapy or at other pathological conditions where CLU has been implicated. |