| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-129 |
Sentence |
denotes |
Screening for noncovalent ligand-receptor interactions by electrospray ionization mass spectrometry-based diffusion measurements. |
| T2 |
130-270 |
Sentence |
denotes |
The application of a novel method for the identification of low-molecular-weight noncovalent ligands to a macromolecular target is reported. |
| T3 |
271-456 |
Sentence |
denotes |
This technique is based on the measurement of analyte diffusion coefficients by electrospray mass spectrometry (ESI-MS) (Clark et al., Rapid Commun. Mass Spectrom. 2002, 16, 1454-1462). |
| T4 |
457-546 |
Sentence |
denotes |
Potential ligands have large diffusion coefficients as long as they are free in solution. |
| T5 |
547-659 |
Sentence |
denotes |
Binding to a macromolecular target, however, drastically reduces the diffusional mobility of any ligand species. |
| T6 |
660-850 |
Sentence |
denotes |
Mixtures containing six different saccharides [ribose, rhamnose, glucose, maltose, maltotriose, and N,N',N''-triacetylchitotriose (NAG(3))] were screened for noncovalent binding to lysozyme. |
| T7 |
851-919 |
Sentence |
denotes |
Of these six compounds, only NAG(3) is known to bind to the protein. |
| T8 |
920-1037 |
Sentence |
denotes |
In "direct" binding tests, NAG(3) shows a significantly reduced diffusion coefficient in the presence of the protein. |
| T9 |
1038-1096 |
Sentence |
denotes |
No changes were observed for any of the other saccharides. |
| T10 |
1097-1306 |
Sentence |
denotes |
In a second set of experiments, the use of a "competition" screening method was explored in which mixtures of candidate saccharides were tested for their ability to displace a reference ligand from the target. |
| T11 |
1307-1531 |
Sentence |
denotes |
The addition of NAG(3)-containing mixtures significantly increased the diffusion coefficient of the reference ligand NAG(4) (N,N',N'',N'''-tetraacetylchitotetrose), whereas mixtures that did not contain NAG(3) had no effect. |
| T12 |
1532-1715 |
Sentence |
denotes |
These data clearly indicate the potential of ESI-MS-based diffusion measurements as a novel tool to screen compound libraries for binding to proteins and other macromolecular targets. |
| T13 |
1716-1881 |
Sentence |
denotes |
In contrast to conventional ESI-MS-based ligand-receptor binding studies, this method does not rely on the preservation of noncovalent interactions in the gas phase. |
