PubMed:14716816
Annnotations
PubMed_Structured_Abstracts
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 200-549 | OBJECTIVE | denotes | To determine the expression levels of three metabolic enzymes of fluoropyrimidines: thymidylate synthase (TS), thymidine phosphorylase (TP) and dihydropyrimidine dehydrogenase (DPD) in seven human gastrointestinal cancer cell lines, and to compare the enzyme levels with the sensitivity to 5-fluorouracil (5-FU) and 5-fluoro-2'-deoxyuridine (FdUrd). |
| T2 | 559-807 | METHODS | denotes | TS, TP and DPD mRNA levels were assessed by semi-quantitative RT-PCR, TP and DPD protein contents were measured by ELISA. Fifty percent inhibitory concentrations of growth (IC50), representing the sensitivity to drugs, were determined by MTT assay. |
| T3 | 817-1280 | RESULTS | denotes | IC50 values ranged from 1.28 to 12.26 microM for 5-FU, and from 5.02 to 24.21 microM for FdUrd, respectively. Cell lines with lower DPD mRNA and protein levels tended to be more sensitive to 5-FU (P<0.05), but neither TS nor TP correlated with 5-FU IC50 (P>0.05). Only TS mRNA level was sharply related with FdUrd sensitivity (P<0.05), but TP and DPD were not (P>0.05). A correlation was found between mRNA and protein levels of DPD (P<0.05), but not TP (P<0.05). |
| T4 | 1293-1411 | CONCLUSIONS | denotes | DPD and TS enzyme levels may be useful indicators in predicting the antitumor activity of 5-FU or FdUrd, respectively. |
PubmedHPO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 414-420 | HP_0002664 | denotes | cancer |
| T2 | 459-486 | HP_0006901 | denotes | levels with the sensitivity |
DisGeNET5_gene_disease
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| 14716816-0#65#96#gene1806 | 65-96 | gene1806 | denotes | dihydropyrimidine dehydrogenase |
| 14716816-0#117#140#diseaseC0685938 | 117-140 | diseaseC0685938 | denotes | gastrointestinal cancer |
| 14716816-1#111#134#gene1890 | 311-334 | gene1890 | denotes | thymidine phosphorylase |
| 14716816-1#136#138#gene1890 | 336-338 | gene1890 | denotes | TP |
| 14716816-1#197#220#diseaseC0685938 | 397-420 | diseaseC0685938 | denotes | gastrointestinal cancer |
| 65#96#gene1806117#140#diseaseC0685938 | 14716816-0#65#96#gene1806 | 14716816-0#117#140#diseaseC0685938 | associated_with | dihydropyrimidine dehydrogenase,gastrointestinal cancer |
| 111#134#gene1890197#220#diseaseC0685938 | 14716816-1#111#134#gene1890 | 14716816-1#197#220#diseaseC0685938 | associated_with | thymidine phosphorylase,gastrointestinal cancer |
| 136#138#gene1890197#220#diseaseC0685938 | 14716816-1#136#138#gene1890 | 14716816-1#197#220#diseaseC0685938 | associated_with | TP,gastrointestinal cancer |
DisGeNET
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T0 | 65-96 | gene:1806 | denotes | dihydropyrimidine dehydrogenase |
| T1 | 117-140 | disease:C0685938 | denotes | gastrointestinal cancer |
| T2 | 344-375 | gene:1806 | denotes | dihydropyrimidine dehydrogenase |
| T3 | 397-420 | disease:C0685938 | denotes | gastrointestinal cancer |
| T4 | 336-338 | gene:1890 | denotes | TP |
| T5 | 397-420 | disease:C0685938 | denotes | gastrointestinal cancer |
| T6 | 311-334 | gene:1890 | denotes | thymidine phosphorylase |
| T7 | 397-420 | disease:C0685938 | denotes | gastrointestinal cancer |
| T8 | 377-380 | gene:1806 | denotes | DPD |
| T9 | 397-420 | disease:C0685938 | denotes | gastrointestinal cancer |
| T10 | 306-308 | gene:7298 | denotes | TS |
| T11 | 397-420 | disease:C0685938 | denotes | gastrointestinal cancer |
| R1 | T0 | T1 | associated_with | dihydropyrimidine dehydrogenase,gastrointestinal cancer |
| R2 | T2 | T3 | associated_with | dihydropyrimidine dehydrogenase,gastrointestinal cancer |
| R3 | T4 | T5 | associated_with | TP,gastrointestinal cancer |
| R4 | T6 | T7 | associated_with | thymidine phosphorylase,gastrointestinal cancer |
| R5 | T8 | T9 | associated_with | DPD,gastrointestinal cancer |
| R6 | T10 | T11 | associated_with | TS,gastrointestinal cancer |