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PubMed:14614020 JSONTXT

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PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 246-428 OBJECTIVE denotes The purpose of this research was to quantitatively analyze tumor-specific overexpression of all ErbB receptors and ErbB4 isoforms in transitional cell carcinoma (TCC) of the bladder.
T2 450-1207 METHODS denotes A real-time reverse transcription-PCR protocol was set up to simultaneously quantitate the mRNA levels of all four of the ErbB receptors and ErbB4 isoforms. Exon-intron structure of the ErbB4 gene was determined for ErbB4 isoform analysis. The assay was validated by analyzing: (a) defined ErbB cDNAs; (b) cell lines transfected with defined ErbB cDNAs; and (c) cancer cell lines with ErbB status controlled by Western blotting. ErbB mRNA expression was quantitated from 29 clinical samples representing TCC, interstitial cystitis, or histologically normal bladder. Cutoff expression levels predicting neoplasia at 95% probability were determined. ErbB expression and amplification was analyzed by immunohistochemistry and chromogenic in situ hybridization.
T3 1217-1747 RESULTS denotes Experiments with control cDNAs and cell lines demonstrated that the assay was both specific and sensitive, and that ErbB mRNA levels closely correlated with protein levels in cancer cell lines. Determination of cutoff expression levels indicated tumor-specific overexpression of ErbB2, ErbB3, and specific ErbB4 isoforms in a subset of TCC patients. Significant overexpression of ErbB mRNAs was also detected in cases without amplification of the respective gene or when the protein product was not localized at the cell membrane.
T4 1760-2028 CONCLUSIONS denotes Bladder cancer patients with tumor-specific overexpression of ErbB receptors or their isoforms were identified. Real-time reverse transcription-PCR could be used for ErbB receptor status quantitation to produce prognostic and predictive information for cancer therapy.

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 305-310 HP_0002664 denotes tumor

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
14614020-1#96#100#gene1956 342-346 gene1956 denotes ErbB
14614020-1#115#120#gene2066 361-366 gene2066 denotes ErbB4
14614020-1#133#160#diseaseC0007138 379-406 diseaseC0007138 denotes transitional cell carcinoma
14614020-1#162#165#diseaseC1861305 408-411 diseaseC1861305 denotes TCC
96#100#gene1956133#160#diseaseC0007138 14614020-1#96#100#gene1956 14614020-1#133#160#diseaseC0007138 associated_with ErbB,transitional cell carcinoma
96#100#gene1956162#165#diseaseC1861305 14614020-1#96#100#gene1956 14614020-1#162#165#diseaseC1861305 associated_with ErbB,TCC
115#120#gene2066133#160#diseaseC0007138 14614020-1#115#120#gene2066 14614020-1#133#160#diseaseC0007138 associated_with ErbB4,transitional cell carcinoma
115#120#gene2066162#165#diseaseC1861305 14614020-1#115#120#gene2066 14614020-1#162#165#diseaseC1861305 associated_with ErbB4,TCC

DisGeNET

Id Subject Object Predicate Lexical cue
T0 194-198 gene:1956 denotes ErbB
T1 220-226 disease:C0006826 denotes cancer
T2 194-198 gene:1956 denotes ErbB
T3 220-226 disease:C1306459 denotes cancer
R1 T0 T1 associated_with ErbB,cancer
R2 T2 T3 associated_with ErbB,cancer