| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-217 |
Sentence |
denotes |
Complete amino acid sequence of the human alpha 5 (IV) collagen chain and identification of a single-base mutation in exon 23 converting glycine 521 in the collagenous domain to cysteine in an Alport syndrome patient. |
| TextSentencer_T2 |
218-424 |
Sentence |
denotes |
We have generated and characterized cDNA clones providing the complete amino acid sequence of the human type IV collagen chain whose gene has been shown to be mutated in X chromosome-linked Alport syndrome. |
| TextSentencer_T3 |
425-486 |
Sentence |
denotes |
The entire translation product has 1,685 amino acid residues. |
| TextSentencer_T4 |
487-733 |
Sentence |
denotes |
There is a 26-residue signal peptide, a 1,430-residue collagenous domain starting with a 14-residue noncollagenous sequence, and a Gly-Xaa-Yaa-repeat sequence interrupted at 22 locations, and a 229-residue carboxyl-terminal noncollagenous domain. |
| TextSentencer_T5 |
734-809 |
Sentence |
denotes |
The calculated molecular weight of the mature alpha 5(IV) chain is 158,303. |
| TextSentencer_T6 |
810-985 |
Sentence |
denotes |
Analysis of genomic DNA from members of a kindred with Alport syndrome revealed a new HindIII cleavage site within the coding sequence of one of the cDNA clones characterized. |
| TextSentencer_T7 |
986-1134 |
Sentence |
denotes |
The proband had a new 1.25-kilobase HindIII fragment and a lack of a 1.35-kilobase fragment, and his mildly affected female cousin had both alleles. |
| TextSentencer_T8 |
1135-1296 |
Sentence |
denotes |
The mutation which was located to exon 23 was sequenced from a polymerase chain reaction-amplified product, and shown to be a G----T change in the coding strand. |
| TextSentencer_T9 |
1297-1359 |
Sentence |
denotes |
The mutation changed the GGT codon of glycine 521 to cysteine. |
| TextSentencer_T10 |
1360-1423 |
Sentence |
denotes |
The same mutation was found in one allele of the female cousin. |
| TextSentencer_T11 |
1424-1493 |
Sentence |
denotes |
The results were confirmed by allele-specific hybridization analyses. |
| T1 |
0-217 |
Sentence |
denotes |
Complete amino acid sequence of the human alpha 5 (IV) collagen chain and identification of a single-base mutation in exon 23 converting glycine 521 in the collagenous domain to cysteine in an Alport syndrome patient. |
| T2 |
218-424 |
Sentence |
denotes |
We have generated and characterized cDNA clones providing the complete amino acid sequence of the human type IV collagen chain whose gene has been shown to be mutated in X chromosome-linked Alport syndrome. |
| T3 |
425-486 |
Sentence |
denotes |
The entire translation product has 1,685 amino acid residues. |
| T4 |
487-733 |
Sentence |
denotes |
There is a 26-residue signal peptide, a 1,430-residue collagenous domain starting with a 14-residue noncollagenous sequence, and a Gly-Xaa-Yaa-repeat sequence interrupted at 22 locations, and a 229-residue carboxyl-terminal noncollagenous domain. |
| T5 |
734-809 |
Sentence |
denotes |
The calculated molecular weight of the mature alpha 5(IV) chain is 158,303. |
| T6 |
810-985 |
Sentence |
denotes |
Analysis of genomic DNA from members of a kindred with Alport syndrome revealed a new HindIII cleavage site within the coding sequence of one of the cDNA clones characterized. |
| T7 |
986-1134 |
Sentence |
denotes |
The proband had a new 1.25-kilobase HindIII fragment and a lack of a 1.35-kilobase fragment, and his mildly affected female cousin had both alleles. |
| T8 |
1135-1296 |
Sentence |
denotes |
The mutation which was located to exon 23 was sequenced from a polymerase chain reaction-amplified product, and shown to be a G----T change in the coding strand. |
| T9 |
1297-1359 |
Sentence |
denotes |
The mutation changed the GGT codon of glycine 521 to cysteine. |
| T10 |
1360-1423 |
Sentence |
denotes |
The same mutation was found in one allele of the female cousin. |
| T11 |
1424-1493 |
Sentence |
denotes |
The results were confirmed by allele-specific hybridization analyses. |