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PubMed:1328224 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-68 Sentence denotes Biochemical properties of the 75-kDa tumor necrosis factor receptor.
T2 69-151 Sentence denotes Characterization of ligand binding, internalization, and receptor phosphorylation.
T3 152-350 Sentence denotes An expression plasmid encoding the human 75-kDa tumor necrosis factor (TNF) type 2 receptor (TNF-R2) was constructed and used to generate a stable human cell line (293/TNF-R2) overexpressing TNF-R2.
T4 351-587 Sentence denotes Ligand binding analysis revealed high affinity binding (Kd = 0.2 nM) with approximately 94,000 +/- 7,500 sites/cell for 125I-TNF-alpha and approximately 5-fold lower affinity for TNF-beta (Kd = 1.1 nM) with 264,000 +/- 2,000 sites/cell.
T5 588-848 Sentence denotes Cross-linking of 125I-TNF-alpha and 125I-TNF-beta to 293/TNF-R2 cells yielded predominant complexes with apparent molecular weights of 211,000 for TNF-alpha and 205,000 and 244,000 for TNF-beta, suggesting these complexes contain two or three TNF-R2 molecules.
T6 849-962 Sentence denotes Immunoprecipitation of TNF-R2 from 32P-labeled 293/TNF-R2 cells demonstrated that the receptor is phosphorylated.
T7 963-1099 Sentence denotes The majority (97%) of 32Pi incorporation was found in serine residues with a very low level of incorporation (3%) in threonine residues.
T8 1100-1210 Sentence denotes TNF-alpha treatment of 293/TNF-R2 cells did not significantly affect the degree or pattern of phosphorylation.
T9 1211-1318 Sentence denotes Cell surface-bound 125I-TNF-alpha was slowly internalized by the 293/TNF-R2 cell line with a t1/2 = 25 min.
T10 1319-1453 Sentence denotes Shedding of the extracellular domain of TNF-R2 was induced by 4 beta-phorbol 12-myristate 13-acetate but not by TNF-alpha or TNF-beta.
T1 0-68 Sentence denotes Biochemical properties of the 75-kDa tumor necrosis factor receptor.
T2 69-151 Sentence denotes Characterization of ligand binding, internalization, and receptor phosphorylation.
T3 152-350 Sentence denotes An expression plasmid encoding the human 75-kDa tumor necrosis factor (TNF) type 2 receptor (TNF-R2) was constructed and used to generate a stable human cell line (293/TNF-R2) overexpressing TNF-R2.
T4 351-587 Sentence denotes Ligand binding analysis revealed high affinity binding (Kd = 0.2 nM) with approximately 94,000 +/- 7,500 sites/cell for 125I-TNF-alpha and approximately 5-fold lower affinity for TNF-beta (Kd = 1.1 nM) with 264,000 +/- 2,000 sites/cell.
T5 588-848 Sentence denotes Cross-linking of 125I-TNF-alpha and 125I-TNF-beta to 293/TNF-R2 cells yielded predominant complexes with apparent molecular weights of 211,000 for TNF-alpha and 205,000 and 244,000 for TNF-beta, suggesting these complexes contain two or three TNF-R2 molecules.
T6 849-962 Sentence denotes Immunoprecipitation of TNF-R2 from 32P-labeled 293/TNF-R2 cells demonstrated that the receptor is phosphorylated.
T7 963-1099 Sentence denotes The majority (97%) of 32Pi incorporation was found in serine residues with a very low level of incorporation (3%) in threonine residues.
T8 1100-1210 Sentence denotes TNF-alpha treatment of 293/TNF-R2 cells did not significantly affect the degree or pattern of phosphorylation.
T9 1211-1318 Sentence denotes Cell surface-bound 125I-TNF-alpha was slowly internalized by the 293/TNF-R2 cell line with a t1/2 = 25 min.
T10 1319-1453 Sentence denotes Shedding of the extracellular domain of TNF-R2 was induced by 4 beta-phorbol 12-myristate 13-acetate but not by TNF-alpha or TNF-beta.

FSU-PRGE

Id Subject Object Predicate Lexical cue
T1 30-67 protein denotes 75-kDa tumor necrosis factor receptor
T2 193-243 protein denotes 75-kDa tumor necrosis factor (TNF) type 2 receptor
T3 245-251 protein denotes TNF-R2
T4 320-326 protein denotes TNF-R2
T5 343-349 protein denotes TNF-R2
T6 476-485 protein denotes TNF-alpha
T7 530-538 protein denotes TNF-beta
T8 610-619 protein denotes TNF-alpha
T9 629-637 protein denotes TNF-beta
T10 645-651 protein denotes TNF-R2
T11 735-744 protein denotes TNF-alpha
T12 773-781 protein denotes TNF-beta
T13 831-837 protein denotes TNF-R2
T14 872-878 protein denotes TNF-R2
T15 900-906 protein denotes TNF-R2
T16 1100-1109 protein denotes TNF-alpha
T17 1127-1133 protein denotes TNF-R2
T18 1235-1244 protein denotes TNF-alpha
T19 1280-1286 protein denotes TNF-R2
T20 1359-1365 protein denotes TNF-R2
T21 1431-1440 protein denotes TNF-alpha
T22 1444-1452 protein denotes TNF-beta

AIMed

Id Subject Object Predicate Lexical cue
T1 200-243 protein denotes tumor necrosis factor (TNF) type 2 receptor
T2 245-251 protein denotes TNF-R2
T3 320-326 protein denotes TNF-R2
T4 343-349 protein denotes TNF-R2
T5 476-485 protein denotes TNF-alpha
T6 530-538 protein denotes TNF-beta
T7 610-619 protein denotes TNF-alpha
T8 629-637 protein denotes TNF-beta
T9 645-651 protein denotes TNF-R2
T10 735-744 protein denotes TNF-alpha
T11 773-781 protein denotes TNF-beta
T12 831-837 protein denotes TNF-R2
T13 872-878 protein denotes TNF-R2
T14 900-906 protein denotes TNF-R2
T15 1100-1109 protein denotes TNF-alpha
T16 1127-1133 protein denotes TNF-R2
T17 1280-1286 protein denotes TNF-R2
T18 1359-1365 protein denotes TNF-R2
T19 1431-1440 protein denotes TNF-alpha
T20 1444-1452 protein denotes TNF-beta

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 37-42 Phenotype denotes tumor HP:0002664
T2 200-205 Phenotype denotes tumor HP:0002664

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 37-42 Disease denotes tumor http://purl.obolibrary.org/obo/MONDO_0005070
T2 200-205 Disease denotes tumor http://purl.obolibrary.org/obo/MONDO_0005070

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 187-192 OrganismTaxon denotes human 9606
T2 299-304 OrganismTaxon denotes human 9606

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 1335-1348 Body_part denotes extracellular http://purl.obolibrary.org/obo/GO_0005576

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 649-657 Cell denotes R2 cells http://purl.obolibrary.org/obo/CL:0000690
T2 904-912 Cell denotes R2 cells http://purl.obolibrary.org/obo/CL:0000690
T3 1131-1139 Cell denotes R2 cells http://purl.obolibrary.org/obo/CL:0000690
T4 1284-1291 Cell denotes R2 cell http://purl.obolibrary.org/obo/CL:0000690