PubMed:1322798 JSONTXT

{"project":"FSU-PRGE","denotations":[{"id":"T1","span":{"begin":42,"end":46},"obj":"protein"},{"id":"T2","span":{"begin":53,"end":78},"obj":"protein"},{"id":"T3","span":{"begin":82,"end":85},"obj":"protein"},{"id":"T4","span":{"begin":161,"end":199},"obj":"protein"},{"id":"T5","span":{"begin":203,"end":207},"obj":"protein"},{"id":"T6","span":{"begin":224,"end":227},"obj":"protein"},{"id":"T7","span":{"begin":327,"end":331},"obj":"protein"},{"id":"T8","span":{"begin":372,"end":405},"obj":"protein"},{"id":"T9","span":{"begin":407,"end":412},"obj":"protein"},{"id":"T10","span":{"begin":418,"end":458},"obj":"protein"},{"id":"T11","span":{"begin":460,"end":466},"obj":"protein"},{"id":"T12","span":{"begin":503,"end":507},"obj":"protein"},{"id":"T13","span":{"begin":587,"end":592},"obj":"protein"},{"id":"T14","span":{"begin":617,"end":622},"obj":"protein"},{"id":"T15","span":{"begin":650,"end":656},"obj":"protein"},{"id":"T16","span":{"begin":658,"end":662},"obj":"protein"},{"id":"T17","span":{"begin":673,"end":679},"obj":"protein"},{"id":"T18","span":{"begin":681,"end":684},"obj":"protein"},{"id":"T19","span":{"begin":798,"end":802},"obj":"protein"},{"id":"T20","span":{"begin":826,"end":829},"obj":"protein"},{"id":"T21","span":{"begin":918,"end":922},"obj":"protein"},{"id":"T22","span":{"begin":926,"end":931},"obj":"protein"},{"id":"T23","span":{"begin":1027,"end":1031},"obj":"protein"},{"id":"T24","span":{"begin":1046,"end":1051},"obj":"protein"},{"id":"T25","span":{"begin":1113,"end":1117},"obj":"protein"},{"id":"T26","span":{"begin":1118,"end":1123},"obj":"protein"},{"id":"T27","span":{"begin":1181,"end":1194},"obj":"protein"},{"id":"T28","span":{"begin":1206,"end":1209},"obj":"protein"}],"text":"The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.\nA cDNA clone encoding a novel, widely expressed protein (called growth factor receptor-bound protein 2 or GRB2) containing one src homology 2 (SH2) domain and two SH3 domains was isolated. Immunoblotting experiments indicate that GRB2 associates with tyrosine-phosphorylated epidermal growth factor receptors (EGFRs) and platelet-derived growth factor receptors (PDGFRs) via its SH2 domain. Interestingly, GRB2 exhibits striking structural and functional homology to the C. elegans protein sem-5. It has been shown that sem-5 and two other genes called let-23 (EGFR like) and let-60 (ras like) lie along the same signal transduction pathway controlling C. elegans vulval induction. To examine whether GRB2 is also a component of ras signaling in mammalian cells, microinjection studies were performed. While injection of GRB2 or H-ras proteins alone into quiescent rat fibroblasts did not have mitogenic effect, microinjection of GRB2 together with H-ras protein stimulated DNA synthesis. These results suggest that GRB2/sem-5 plays a crucial role in a highly conserved mechanism for growth factor control of ras signaling."}

{"project":"AIMed","denotations":[{"id":"T1","span":{"begin":42,"end":46},"obj":"protein"},{"id":"T2","span":{"begin":161,"end":199},"obj":"protein"},{"id":"T3","span":{"begin":203,"end":207},"obj":"protein"},{"id":"T4","span":{"begin":327,"end":331},"obj":"protein"},{"id":"T5","span":{"begin":503,"end":507},"obj":"protein"},{"id":"T6","span":{"begin":798,"end":802},"obj":"protein"},{"id":"T7","span":{"begin":918,"end":922},"obj":"protein"},{"id":"T8","span":{"begin":926,"end":931},"obj":"protein"},{"id":"T9","span":{"begin":1027,"end":1031},"obj":"protein"},{"id":"T10","span":{"begin":1046,"end":1051},"obj":"protein"},{"id":"T11","span":{"begin":1113,"end":1117},"obj":"protein"},{"id":"T12","span":{"begin":1118,"end":1123},"obj":"protein"}],"text":"The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.\nA cDNA clone encoding a novel, widely expressed protein (called growth factor receptor-bound protein 2 or GRB2) containing one src homology 2 (SH2) domain and two SH3 domains was isolated. Immunoblotting experiments indicate that GRB2 associates with tyrosine-phosphorylated epidermal growth factor receptors (EGFRs) and platelet-derived growth factor receptors (PDGFRs) via its SH2 domain. Interestingly, GRB2 exhibits striking structural and functional homology to the C. elegans protein sem-5. It has been shown that sem-5 and two other genes called let-23 (EGFR like) and let-60 (ras like) lie along the same signal transduction pathway controlling C. elegans vulval induction. To examine whether GRB2 is also a component of ras signaling in mammalian cells, microinjection studies were performed. While injection of GRB2 or H-ras proteins alone into quiescent rat fibroblasts did not have mitogenic effect, microinjection of GRB2 together with H-ras protein stimulated DNA synthesis. These results suggest that GRB2/sem-5 plays a crucial role in a highly conserved mechanism for growth factor control of ras signaling."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":962,"end":965},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"10114"},{"id":"A2","pred":"db_id","subj":"T1","obj":"10116"}],"text":"The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.\nA cDNA clone encoding a novel, widely expressed protein (called growth factor receptor-bound protein 2 or GRB2) containing one src homology 2 (SH2) domain and two SH3 domains was isolated. Immunoblotting experiments indicate that GRB2 associates with tyrosine-phosphorylated epidermal growth factor receptors (EGFRs) and platelet-derived growth factor receptors (PDGFRs) via its SH2 domain. Interestingly, GRB2 exhibits striking structural and functional homology to the C. elegans protein sem-5. It has been shown that sem-5 and two other genes called let-23 (EGFR like) and let-60 (ras like) lie along the same signal transduction pathway controlling C. elegans vulval induction. To examine whether GRB2 is also a component of ras signaling in mammalian cells, microinjection studies were performed. While injection of GRB2 or H-ras proteins alone into quiescent rat fibroblasts did not have mitogenic effect, microinjection of GRB2 together with H-ras protein stimulated DNA synthesis. These results suggest that GRB2/sem-5 plays a crucial role in a highly conserved mechanism for growth factor control of ras signaling."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":372,"end":388},"obj":"Body_part"},{"id":"T2","span":{"begin":418,"end":426},"obj":"Body_part"},{"id":"T3","span":{"begin":966,"end":977},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000021"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL_0000233"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000057"}],"text":"The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.\nA cDNA clone encoding a novel, widely expressed protein (called growth factor receptor-bound protein 2 or GRB2) containing one src homology 2 (SH2) domain and two SH3 domains was isolated. Immunoblotting experiments indicate that GRB2 associates with tyrosine-phosphorylated epidermal growth factor receptors (EGFRs) and platelet-derived growth factor receptors (PDGFRs) via its SH2 domain. Interestingly, GRB2 exhibits striking structural and functional homology to the C. elegans protein sem-5. It has been shown that sem-5 and two other genes called let-23 (EGFR like) and let-60 (ras like) lie along the same signal transduction pathway controlling C. elegans vulval induction. To examine whether GRB2 is also a component of ras signaling in mammalian cells, microinjection studies were performed. While injection of GRB2 or H-ras proteins alone into quiescent rat fibroblasts did not have mitogenic effect, microinjection of GRB2 together with H-ras protein stimulated DNA synthesis. These results suggest that GRB2/sem-5 plays a crucial role in a highly conserved mechanism for growth factor control of ras signaling."}

{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":418,"end":426},"obj":"Cell"},{"id":"T2","span":{"begin":966,"end":977},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000233"},{"id":"A2","pred":"cl_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL:0000057"}],"text":"The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.\nA cDNA clone encoding a novel, widely expressed protein (called growth factor receptor-bound protein 2 or GRB2) containing one src homology 2 (SH2) domain and two SH3 domains was isolated. Immunoblotting experiments indicate that GRB2 associates with tyrosine-phosphorylated epidermal growth factor receptors (EGFRs) and platelet-derived growth factor receptors (PDGFRs) via its SH2 domain. Interestingly, GRB2 exhibits striking structural and functional homology to the C. elegans protein sem-5. It has been shown that sem-5 and two other genes called let-23 (EGFR like) and let-60 (ras like) lie along the same signal transduction pathway controlling C. elegans vulval induction. To examine whether GRB2 is also a component of ras signaling in mammalian cells, microinjection studies were performed. While injection of GRB2 or H-ras proteins alone into quiescent rat fibroblasts did not have mitogenic effect, microinjection of GRB2 together with H-ras protein stimulated DNA synthesis. These results suggest that GRB2/sem-5 plays a crucial role in a highly conserved mechanism for growth factor control of ras signaling."}