PubMed:1316705 JSONTXT

{"project":"AnEM_abstracts","denotations":[{"id":"T1","span":{"begin":0,"end":10},"obj":"Cell"},{"id":"T2","span":{"begin":31,"end":43},"obj":"Cell"},{"id":"T3","span":{"begin":64,"end":75},"obj":"Anatomical_system"},{"id":"T4","span":{"begin":151,"end":162},"obj":"Cell"},{"id":"T5","span":{"begin":244,"end":256},"obj":"Cell"},{"id":"T6","span":{"begin":279,"end":290},"obj":"Cell"},{"id":"T7","span":{"begin":686,"end":692},"obj":"Organism_substance"},{"id":"T8","span":{"begin":934,"end":945},"obj":"Cell"}],"text":"Neutrophil function in chronic neutrophilic leukemia: defective respiratory burst in response to phorbol esters.\nFunctional analyses were performed on neutrophils isolated from 6 patients from two institutions who displayed features of chronic neutrophilic leukemia (CNL). These neutrophils demonstrated a consistent deficiency (44 +/- 8% of control values) in superoxide anion (O2-) production in response to the phorbol ester, phorbol myristate acetate (PMA). O2- production in response to chemotactic peptides was near normal (82.3 +/- 10.7% of control values). Bacterial killing was normal in the two patients studied, and chemotaxis was diminished in response to zymosan-activated plasma and to high concentrations of chemotactic peptides in the patients studied. Cytosolic C kinase activity was decreased in one of the two patients studied. These results suggest that a deficient O2- release in response to PMA is a hallmark of neutrophils in CNL and may provide a diagnostic indicator of this condition."}