PubMed:12914241 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/12914241","sourcedb":"PubMed","sourceid":"12914241","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/12914241","text":"[Deletion mutation of beta-amyloid precursor protein decreases the secretion of beta-amyloid in SH-SY5Y neuroblastoma cells].\nOBJECTIVE: To explore the effect of A beta secretion lacking exons 2 and 3 of APP695 (delta APP695) on SH-SY5Y neuroblastoma cells.\nMETHODS: APP695 cDNA and delta APP695 cDNA eukaryotic expression vectors were constructed by gene recombination. Immunoprecipitation Western blotting was used to analyse the A beta secretion and MTT assay to observe the cell viability's change after being stably transfected with the plasmids by lipofectamine method.\nRESULTS: Stable transfectants were selected in 0.6 g.L-1 G418. delta APP695 cDNA construct transfection decreased significantly the production of beta amyloid in the SH-SY5Y compared with APP695 cDNA construct. It did not affect the cell viability by MTT method.\nCONCLUSION: This result suggests that the alternative splicing of APP exons 2 and 3 modulates the secretion of A beta by influencing the processing of APP.","tracks":[{"project":"PennBioIE","denotations":[{"id":"T1","span":{"begin":1,"end":9},"obj":"protein"},{"id":"T2","span":{"begin":22,"end":52},"obj":"protein"},{"id":"T3","span":{"begin":80,"end":92},"obj":"protein"},{"id":"T4","span":{"begin":104,"end":117},"obj":"protein"},{"id":"T5","span":{"begin":162,"end":168},"obj":"protein"},{"id":"T6","span":{"begin":187,"end":192},"obj":"protein"},{"id":"T7","span":{"begin":199,"end":200},"obj":"protein"},{"id":"T8","span":{"begin":204,"end":210},"obj":"protein"},{"id":"T9","span":{"begin":212,"end":224},"obj":"protein"},{"id":"T10","span":{"begin":237,"end":250},"obj":"protein"},{"id":"T11","span":{"begin":267,"end":273},"obj":"protein"},{"id":"T12","span":{"begin":283,"end":295},"obj":"protein"},{"id":"T13","span":{"begin":432,"end":438},"obj":"protein"},{"id":"T14","span":{"begin":639,"end":651},"obj":"protein"},{"id":"T15","span":{"begin":722,"end":734},"obj":"protein"},{"id":"T16","span":{"begin":764,"end":770},"obj":"protein"},{"id":"T17","span":{"begin":905,"end":908},"obj":"protein"},{"id":"T18","span":{"begin":909,"end":914},"obj":"protein"},{"id":"T19","span":{"begin":921,"end":922},"obj":"protein"},{"id":"T20","span":{"begin":950,"end":956},"obj":"protein"},{"id":"T21","span":{"begin":990,"end":993},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"PennBioIE"},{"subj":"T2","pred":"source","obj":"PennBioIE"},{"subj":"T3","pred":"source","obj":"PennBioIE"},{"subj":"T4","pred":"source","obj":"PennBioIE"},{"subj":"T5","pred":"source","obj":"PennBioIE"},{"subj":"T6","pred":"source","obj":"PennBioIE"},{"subj":"T7","pred":"source","obj":"PennBioIE"},{"subj":"T8","pred":"source","obj":"PennBioIE"},{"subj":"T9","pred":"source","obj":"PennBioIE"},{"subj":"T10","pred":"source","obj":"PennBioIE"},{"subj":"T11","pred":"source","obj":"PennBioIE"},{"subj":"T12","pred":"source","obj":"PennBioIE"},{"subj":"T13","pred":"source","obj":"PennBioIE"},{"subj":"T14","pred":"source","obj":"PennBioIE"},{"subj":"T15","pred":"source","obj":"PennBioIE"},{"subj":"T16","pred":"source","obj":"PennBioIE"},{"subj":"T17","pred":"source","obj":"PennBioIE"},{"subj":"T18","pred":"source","obj":"PennBioIE"},{"subj":"T19","pred":"source","obj":"PennBioIE"},{"subj":"T20","pred":"source","obj":"PennBioIE"},{"subj":"T21","pred":"source","obj":"PennBioIE"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PennBioIE","color":"#b6ec93","default":true}]}]}}