PubMed:12785846 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/12785846","sourcedb":"PubMed","sourceid":"12785846","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/12785846","text":"Chemoselective ligation applied to the synthesis of a biantennary N-linked glycoform of CD52.\nWe report here a strategy for the synthesis of N-linked glycopeptide analogues that replace the glycosidic linkages extending from the core pentasaccharide with thioethers amenable to construction by chemoselective ligation. The key building block, a pentasaccharide-Asn analogue containing two thiol residues, was incorporated into CD52 by 9-fluorenylmethoxycarbonyl (Fmoc)-based solid-phase peptide synthesis. An undecasaccharide mimetic was then readily generated by alkylation of this glycopeptide with an N-bromoacetamido trisaccharide. The rapid assembly of a complex type N-linked glycopeptide mimetic was accomplished using this technique.","tracks":[]}