| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-137 |
Sentence |
denotes |
Identification of an equilibrium intermediate in the unfolding process of galectin-1, which retains its carbohydrate-binding specificity. |
| T2 |
138-358 |
Sentence |
denotes |
The unfolding process of galectin-1 (Gal-1) in the presence of a denaturing agent was examined using fluorescence and far-UV circular dichroism (CD) spectroscopy determinations, and was found to be completely reversible. |
| T3 |
359-575 |
Sentence |
denotes |
The data showed that the transitions of guanidine hydrochloride (GdnHCl)-induced lectin unfolding, in the absence of ligand, were biphasic in nature, clearly showing the existence of at least one stable intermediate. |
| T4 |
576-747 |
Sentence |
denotes |
On the other hand, the unfolding in the presence of disaccharide yielded data that could fit very well to a two-state model, indicating a stabilizing effect of the ligand. |
| T5 |
748-932 |
Sentence |
denotes |
The folding intermediate was further characterized by size exclusion chromatography, near-UV CD and anilinonaphtalene sulfonate binding, and shown to belong to the molten globule type. |
| T6 |
933-1105 |
Sentence |
denotes |
Strikingly, this intermediate retained its carbohydrate-binding specificity, as evidenced by the tryptophan fluorescence changes detected upon its interaction with lactose. |
