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Inflammaging

Id Subject Object Predicate Lexical cue
T1 0-242 Sentence denotes Responses to the proinflammatory cytokines interleukin-1 and tumor necrosis factor alpha in cells derived from rheumatoid synovium and other joint tissues involve nuclear factor kappaB-mediated induction of the Ets transcription factor ESE-1.
T2 243-253 Sentence denotes OBJECTIVE:
T3 254-559 Sentence denotes To investigate the expression of the novel Ets transcription factor ESE-1 in rheumatoid synovium and in cells derived from joint tissues, and to analyze the role of nuclear factor kappaB (NF-kappaB) as one of the central downstream targets in mediating the induction of ESE-1 by proinflammatory cytokines.
T4 560-737 Sentence denotes METHODS: ESE-1 protein expression was analyzed by immunohistochemistry using antibodies in synovial tissues from patients with rheumatoid arthritis (RA) and osteoarthritis (OA).
T5 738-1169 Sentence denotes ESE-1 messenger RNA (mRNA) levels were analyzed by reverse transcriptase-polymerase chain reaction or Northern blotting in human chondrocytes, synovial fibroblasts, osteoblasts, and macrophages, before and after exposure to interleukin-1beta (IL-1beta), tumor necrosis factor alpha (TNFalpha), or lipopolysaccharide (LPS) with or without prior infection with an adenovirus encoding the inhibitor of nuclear factor kappaB (IkappaB).
T6 1170-1338 Sentence denotes The wild-type ESE-1 promoter and the ESE-1 promoter mutated in the NF-kappaB site were cloned into a luciferase reporter vector and analyzed in transient transfections.
T7 1339-1538 Sentence denotes Electrophoretic mobility shift assays (EMSAs) and supershift assays with antibodies against members of the NF-kappaB family were conducted using the NF-kappaB site from the ESE-1 promoter as a probe.
T8 1539-1547 Sentence denotes RESULTS:
T9 1548-1727 Sentence denotes Immunohistochemical analysis showed specific expression of ESE-1 in cells of the synovial lining layer and in some mononuclear and endothelial cells in RA and OA synovial tissues.
T10 1728-1872 Sentence denotes ESE-1 mRNA expression could be induced by IL-1beta and TNFalpha in cells such as synovial fibroblasts, chondrocytes, osteoblasts, and monocytes.
T11 1873-2096 Sentence denotes Transient transfection experiments and EMSAs showed that induction of ESE-1 gene expression by IL-1beta requires activation of NF-kappaB and binding of p50 and p65 family members to the NF-kappaB site in the ESE-1 promoter.
T12 2097-2197 Sentence denotes Overexpression of IkappaB using an adenoviral vector blocked IL-1beta-induced ESE-1 mRNA expression.
T13 2198-2297 Sentence denotes Chromatin immunoprecipitation further confirmed that NF-kappaB binds to the ESE-1 promoter in vivo.
T14 2298-2524 Sentence denotes CONCLUSION: ESE-1 is expressed in synovial tissues in RA and, to a variable extent, in OA, and is specifically induced in synovial fibroblasts, chondrocytes, osteoblasts, and monocyte/macrophages by IL-1beta, TNFalpha, or LPS.
T15 2525-2712 Sentence denotes This induction relies on the translocation of the NF-kappaB family members p50 and p65 to the nucleus and transactivation of the ESE-1 promoter via a high-affinity NF-kappaB binding site.
T16 2713-2822 Sentence denotes ESE-1 may play a role in mediating some effects of proinflammatory stimuli in cells at sites of inflammation.
T1 0-242 Sentence denotes Responses to the proinflammatory cytokines interleukin-1 and tumor necrosis factor alpha in cells derived from rheumatoid synovium and other joint tissues involve nuclear factor kappaB-mediated induction of the Ets transcription factor ESE-1.
T2 243-253 Sentence denotes OBJECTIVE:
T3 254-559 Sentence denotes To investigate the expression of the novel Ets transcription factor ESE-1 in rheumatoid synovium and in cells derived from joint tissues, and to analyze the role of nuclear factor kappaB (NF-kappaB) as one of the central downstream targets in mediating the induction of ESE-1 by proinflammatory cytokines.
T4 560-737 Sentence denotes METHODS: ESE-1 protein expression was analyzed by immunohistochemistry using antibodies in synovial tissues from patients with rheumatoid arthritis (RA) and osteoarthritis (OA).
T5 738-1169 Sentence denotes ESE-1 messenger RNA (mRNA) levels were analyzed by reverse transcriptase-polymerase chain reaction or Northern blotting in human chondrocytes, synovial fibroblasts, osteoblasts, and macrophages, before and after exposure to interleukin-1beta (IL-1beta), tumor necrosis factor alpha (TNFalpha), or lipopolysaccharide (LPS) with or without prior infection with an adenovirus encoding the inhibitor of nuclear factor kappaB (IkappaB).
T6 1170-1338 Sentence denotes The wild-type ESE-1 promoter and the ESE-1 promoter mutated in the NF-kappaB site were cloned into a luciferase reporter vector and analyzed in transient transfections.
T7 1339-1538 Sentence denotes Electrophoretic mobility shift assays (EMSAs) and supershift assays with antibodies against members of the NF-kappaB family were conducted using the NF-kappaB site from the ESE-1 promoter as a probe.
T8 1539-1547 Sentence denotes RESULTS:
T9 1548-1727 Sentence denotes Immunohistochemical analysis showed specific expression of ESE-1 in cells of the synovial lining layer and in some mononuclear and endothelial cells in RA and OA synovial tissues.
T10 1728-1872 Sentence denotes ESE-1 mRNA expression could be induced by IL-1beta and TNFalpha in cells such as synovial fibroblasts, chondrocytes, osteoblasts, and monocytes.
T11 1873-2096 Sentence denotes Transient transfection experiments and EMSAs showed that induction of ESE-1 gene expression by IL-1beta requires activation of NF-kappaB and binding of p50 and p65 family members to the NF-kappaB site in the ESE-1 promoter.
T12 2097-2197 Sentence denotes Overexpression of IkappaB using an adenoviral vector blocked IL-1beta-induced ESE-1 mRNA expression.
T13 2198-2297 Sentence denotes Chromatin immunoprecipitation further confirmed that NF-kappaB binds to the ESE-1 promoter in vivo.
T14 2298-2524 Sentence denotes CONCLUSION: ESE-1 is expressed in synovial tissues in RA and, to a variable extent, in OA, and is specifically induced in synovial fibroblasts, chondrocytes, osteoblasts, and monocyte/macrophages by IL-1beta, TNFalpha, or LPS.
T15 2525-2712 Sentence denotes This induction relies on the translocation of the NF-kappaB family members p50 and p65 to the nucleus and transactivation of the ESE-1 promoter via a high-affinity NF-kappaB binding site.
T16 2713-2822 Sentence denotes ESE-1 may play a role in mediating some effects of proinflammatory stimuli in cells at sites of inflammation.

PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 254-559 OBJECTIVE denotes To investigate the expression of the novel Ets transcription factor ESE-1 in rheumatoid synovium and in cells derived from joint tissues, and to analyze the role of nuclear factor kappaB (NF-kappaB) as one of the central downstream targets in mediating the induction of ESE-1 by proinflammatory cytokines.
T2 569-1538 METHODS denotes ESE-1 protein expression was analyzed by immunohistochemistry using antibodies in synovial tissues from patients with rheumatoid arthritis (RA) and osteoarthritis (OA). ESE-1 messenger RNA (mRNA) levels were analyzed by reverse transcriptase-polymerase chain reaction or Northern blotting in human chondrocytes, synovial fibroblasts, osteoblasts, and macrophages, before and after exposure to interleukin-1beta (IL-1beta), tumor necrosis factor alpha (TNFalpha), or lipopolysaccharide (LPS) with or without prior infection with an adenovirus encoding the inhibitor of nuclear factor kappaB (IkappaB). The wild-type ESE-1 promoter and the ESE-1 promoter mutated in the NF-kappaB site were cloned into a luciferase reporter vector and analyzed in transient transfections. Electrophoretic mobility shift assays (EMSAs) and supershift assays with antibodies against members of the NF-kappaB family were conducted using the NF-kappaB site from the ESE-1 promoter as a probe.
T3 1548-2297 RESULTS denotes Immunohistochemical analysis showed specific expression of ESE-1 in cells of the synovial lining layer and in some mononuclear and endothelial cells in RA and OA synovial tissues. ESE-1 mRNA expression could be induced by IL-1beta and TNFalpha in cells such as synovial fibroblasts, chondrocytes, osteoblasts, and monocytes. Transient transfection experiments and EMSAs showed that induction of ESE-1 gene expression by IL-1beta requires activation of NF-kappaB and binding of p50 and p65 family members to the NF-kappaB site in the ESE-1 promoter. Overexpression of IkappaB using an adenoviral vector blocked IL-1beta-induced ESE-1 mRNA expression. Chromatin immunoprecipitation further confirmed that NF-kappaB binds to the ESE-1 promoter in vivo.
T4 2310-2822 CONCLUSIONS denotes ESE-1 is expressed in synovial tissues in RA and, to a variable extent, in OA, and is specifically induced in synovial fibroblasts, chondrocytes, osteoblasts, and monocyte/macrophages by IL-1beta, TNFalpha, or LPS. This induction relies on the translocation of the NF-kappaB family members p50 and p65 to the nucleus and transactivation of the ESE-1 promoter via a high-affinity NF-kappaB binding site. ESE-1 may play a role in mediating some effects of proinflammatory stimuli in cells at sites of inflammation.

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
12746898-11#0#5#gene1999 2310-2315 gene1999 denotes ESE-1
12746898-11#197#205#gene7124 2507-2515 gene7124 denotes TNFalpha
12746898-11#42#44#diseaseC0003873 2352-2354 diseaseC0003873 denotes RA
12746898-11#75#77#diseaseC0029408 2385-2387 diseaseC0029408 denotes OA
12746898-11#42#44#diseaseC0003873 2352-2354 diseaseC0003873 denotes RA
0#5#gene199942#44#diseaseC0003873 12746898-11#0#5#gene1999 12746898-11#42#44#diseaseC0003873 associated_with ESE-1,RA
0#5#gene199975#77#diseaseC0029408 12746898-11#0#5#gene1999 12746898-11#75#77#diseaseC0029408 associated_with ESE-1,OA
0#5#gene199942#44#diseaseC0003873 12746898-11#0#5#gene1999 12746898-11#42#44#diseaseC0003873 associated_with ESE-1,RA
197#205#gene712442#44#diseaseC0003873 12746898-11#197#205#gene7124 12746898-11#42#44#diseaseC0003873 associated_with TNFalpha,RA
197#205#gene712475#77#diseaseC0029408 12746898-11#197#205#gene7124 12746898-11#75#77#diseaseC0029408 associated_with TNFalpha,OA
197#205#gene712442#44#diseaseC0003873 12746898-11#197#205#gene7124 12746898-11#42#44#diseaseC0003873 associated_with TNFalpha,RA

DisGeNET

Id Subject Object Predicate Lexical cue
T0 1607-1612 gene:1999 denotes ESE-1
T1 1700-1702 disease:C0003873 denotes RA
T2 1607-1612 gene:1999 denotes ESE-1
T3 1707-1709 disease:C0029408 denotes OA
R1 T0 T1 associated_with ESE-1,RA
R2 T2 T3 associated_with ESE-1,OA