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PubMed:12732618 JSONTXT

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GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 65-80 Glycan_Motif denotes keratan sulfate https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82109MW
T2 152-167 Glycan_Motif denotes Keratan sulfate https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82109MW
T3 169-171 Glycan_Motif denotes KS https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82109MW
T4 316-318 Glycan_Motif denotes KS https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82109MW
T5 340-342 Glycan_Motif denotes KS https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82109MW
T6 702-704 Glycan_Motif denotes KS https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82109MW
T7 791-801 Glycan_Motif denotes hyaluronan https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00017MO
T8 820-827 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO
T10 833-844 Glycan_Motif denotes chondroitin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT
T11 894-896 Glycan_Motif denotes KS https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82109MW
T12 934-936 Glycan_Motif denotes KS https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82109MW
T13 993-995 Glycan_Motif denotes KS https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82109MW
T14 1058-1060 Glycan_Motif denotes KS https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82109MW
T15 1142-1144 Glycan_Motif denotes KS https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82109MW
T16 1680-1682 Glycan_Motif denotes KS https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82109MW
T17 1817-1819 Glycan_Motif denotes KS https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82109MW

sentences

Id Subject Object Predicate Lexical cue
T1 0-151 Sentence denotes Purification, characterization, and molecular cloning of a novel keratan sulfate hydrolase, endo-beta-N-acetylglucosaminidase, from Bacillus circulans.
T2 152-319 Sentence denotes Keratan sulfate (KS) is degraded by various enzymes including endo-beta-galactosidase, keratanase, and keratanase II, which are used for the structural analysis of KS.
T3 320-608 Sentence denotes We purified a novel KS hydrolase, endo-beta-N-acetylglucosaminidase, from the cell pellet and conditioned medium of Bacillus circulans, by sequential chromatography using DE52 and phenyl-Sepharose columns with approximately 63- and 180-fold purity and 58 and 12.5% recovery, respectively.
T4 609-643 Sentence denotes Like keratanase II of Bacillus sp.
T5 644-897 Sentence denotes Ks36, the enzyme, designated Bc keratanase II, hydrolyzed KS between the 4GlcNAcbeta1-3Gal1 structure (endo-beta-N-acetylglucosaminidase), but not hyaluronan, heparan sulfate, heparin, and chondroitin sulfate C, demonstrating a strict specificity to KS.
T6 898-1061 Sentence denotes The enzyme digested shark cartilage KS to disaccharides and tetrasaccharides and bovine cornea KS to hexasaccharide, indicating that it prefers highly sulfated KS.
T7 1062-1187 Sentence denotes Distinct from keratanase II of strain Ks36, the enzyme digested shark cartilage KS at an optimal temperature of 55 degrees C.
T8 1188-1352 Sentence denotes Based on partial peptide sequencing of the enzyme, we molecularly cloned the gene, which encodes a protein with a predicted molecular mass of approximately 200 kDa.
T9 1353-1690 Sentence denotes From the deduced protein sequence, Bc keratanase II contained a domain at the C terminus, homologous to the S-layer-like domain of pullulanase from Thermoanaerobacterium thermosulfurigenes and endoxylanase from Thermoanaerobacterium saccharolyticum, and a carbohydrate-binding domain, which may serve to specifically recognize KS chains.
T10 1691-1754 Sentence denotes A full-length recombinant enzyme showed keratanase II activity.
T11 1755-1870 Sentence denotes These results may prove useful for the structural analysis of KS toward achieving an understanding of its function.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 65-80 https://glytoucan.org/Structures/Glycans/G82109MW denotes keratan sulfate
T2 152-167 https://glytoucan.org/Structures/Glycans/G82109MW denotes Keratan sulfate
T3 791-801 https://glytoucan.org/Structures/Glycans/G00017MO denotes hyaluronan
T4 820-827 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T5 820-827 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T6 833-844 https://glytoucan.org/Structures/Glycans/G43702JT denotes chondroitin

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 924-933 http://purl.obolibrary.org/obo/MAT_0000189 denotes cartilage
T2 1132-1141 http://purl.obolibrary.org/obo/MAT_0000189 denotes cartilage

Glycosmos6-GlycoEpitope

Id Subject Object Predicate Lexical cue
T1 65-80 http://www.glycoepitope.jp/epitopes/EP0085 denotes keratan sulfate
T2 152-167 http://www.glycoepitope.jp/epitopes/EP0085 denotes Keratan sulfate
T3 169-171 http://www.glycoepitope.jp/epitopes/EP0085 denotes KS
T4 316-318 http://www.glycoepitope.jp/epitopes/EP0085 denotes KS
T5 340-342 http://www.glycoepitope.jp/epitopes/EP0085 denotes KS
T6 702-704 http://www.glycoepitope.jp/epitopes/EP0085 denotes KS
T7 803-818 http://www.glycoepitope.jp/epitopes/EP0086 denotes heparan sulfate
T8 833-844 http://www.glycoepitope.jp/epitopes/EP0081 denotes chondroitin
T9 894-896 http://www.glycoepitope.jp/epitopes/EP0085 denotes KS
T10 934-936 http://www.glycoepitope.jp/epitopes/EP0085 denotes KS
T11 993-995 http://www.glycoepitope.jp/epitopes/EP0085 denotes KS
T12 1058-1060 http://www.glycoepitope.jp/epitopes/EP0085 denotes KS
T13 1142-1144 http://www.glycoepitope.jp/epitopes/EP0085 denotes KS
T14 1680-1682 http://www.glycoepitope.jp/epitopes/EP0085 denotes KS
T15 1817-1819 http://www.glycoepitope.jp/epitopes/EP0085 denotes KS

NGLY1-deficiency

Id Subject Object Predicate Lexical cue
PD-NGLY1-deficiency-B_T1 92-125 hgnc:24622 denotes endo-beta-N-acetylglucosaminidase
PD-NGLY1-deficiency-B_T2 354-387 hgnc:24622 denotes endo-beta-N-acetylglucosaminidase
PD-NGLY1-deficiency-B_T3 747-780 hgnc:24622 denotes endo-beta-N-acetylglucosaminidase

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 924-933 Body_part denotes cartilage http://purl.obolibrary.org/obo/MAT_0000189
T2 1132-1141 Body_part denotes cartilage http://purl.obolibrary.org/obo/MAT_0000189

GlyCosmos-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 65-80 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes keratan sulfate http://www.glycoepitope.jp/epitopes/EP0085
T2 152-167 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes Keratan sulfate http://www.glycoepitope.jp/epitopes/EP0085
T3 169-171 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T4 316-318 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T5 340-342 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T6 702-704 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T7 803-818 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes heparan sulfate http://www.glycoepitope.jp/epitopes/EP0086
T8 833-844 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes chondroitin http://www.glycoepitope.jp/epitopes/EP0081
T9 894-896 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T10 934-936 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T11 993-995 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T12 1058-1060 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T13 1142-1144 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T14 1680-1682 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T15 1817-1819 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085

GlyCosmos15-NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 132-140 OrganismTaxon denotes Bacillus 1386|55087
T3 436-444 OrganismTaxon denotes Bacillus 1386|55087
T5 631-639 OrganismTaxon denotes Bacillus 1386|55087
T7 979-985 OrganismTaxon denotes bovine 9913
T8 1501-1541 OrganismTaxon denotes Thermoanaerobacterium thermosulfurigenes 33950
T9 1564-1601 OrganismTaxon denotes Thermoanaerobacterium saccharolyticum 28896

GlyCosmos15-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 403-409 Body_part denotes pellet http://purl.obolibrary.org/obo/UBERON_0036018
T2 924-933 Body_part denotes cartilage http://purl.obolibrary.org/obo/UBERON_0002418|http://purl.obolibrary.org/obo/UBERON_0007844
T4 986-992 Body_part denotes cornea http://purl.obolibrary.org/obo/UBERON_0000964
T5 1132-1141 Body_part denotes cartilage http://purl.obolibrary.org/obo/UBERON_0002418|http://purl.obolibrary.org/obo/UBERON_0007844
T7 1463-1468 Body_part denotes layer http://purl.obolibrary.org/obo/UBERON_0000119|http://purl.obolibrary.org/obo/UBERON_0022303

GlyCosmos15-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 924-933 Body_part denotes cartilage http://purl.obolibrary.org/obo/MAT_0000189
T2 1132-1141 Body_part denotes cartilage http://purl.obolibrary.org/obo/MAT_0000189

sentences

Id Subject Object Predicate Lexical cue
T1 0-151 Sentence denotes Purification, characterization, and molecular cloning of a novel keratan sulfate hydrolase, endo-beta-N-acetylglucosaminidase, from Bacillus circulans.
T2 152-319 Sentence denotes Keratan sulfate (KS) is degraded by various enzymes including endo-beta-galactosidase, keratanase, and keratanase II, which are used for the structural analysis of KS.
T3 320-608 Sentence denotes We purified a novel KS hydrolase, endo-beta-N-acetylglucosaminidase, from the cell pellet and conditioned medium of Bacillus circulans, by sequential chromatography using DE52 and phenyl-Sepharose columns with approximately 63- and 180-fold purity and 58 and 12.5% recovery, respectively.
T4 609-643 Sentence denotes Like keratanase II of Bacillus sp.
T5 644-897 Sentence denotes Ks36, the enzyme, designated Bc keratanase II, hydrolyzed KS between the 4GlcNAcbeta1-3Gal1 structure (endo-beta-N-acetylglucosaminidase), but not hyaluronan, heparan sulfate, heparin, and chondroitin sulfate C, demonstrating a strict specificity to KS.
T6 898-1061 Sentence denotes The enzyme digested shark cartilage KS to disaccharides and tetrasaccharides and bovine cornea KS to hexasaccharide, indicating that it prefers highly sulfated KS.
T7 1062-1187 Sentence denotes Distinct from keratanase II of strain Ks36, the enzyme digested shark cartilage KS at an optimal temperature of 55 degrees C.
T8 1188-1352 Sentence denotes Based on partial peptide sequencing of the enzyme, we molecularly cloned the gene, which encodes a protein with a predicted molecular mass of approximately 200 kDa.
T9 1353-1690 Sentence denotes From the deduced protein sequence, Bc keratanase II contained a domain at the C terminus, homologous to the S-layer-like domain of pullulanase from Thermoanaerobacterium thermosulfurigenes and endoxylanase from Thermoanaerobacterium saccharolyticum, and a carbohydrate-binding domain, which may serve to specifically recognize KS chains.
T10 1691-1754 Sentence denotes A full-length recombinant enzyme showed keratanase II activity.
T11 1755-1870 Sentence denotes These results may prove useful for the structural analysis of KS toward achieving an understanding of its function.

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-151 Sentence denotes Purification, characterization, and molecular cloning of a novel keratan sulfate hydrolase, endo-beta-N-acetylglucosaminidase, from Bacillus circulans.
T2 152-319 Sentence denotes Keratan sulfate (KS) is degraded by various enzymes including endo-beta-galactosidase, keratanase, and keratanase II, which are used for the structural analysis of KS.
T3 320-608 Sentence denotes We purified a novel KS hydrolase, endo-beta-N-acetylglucosaminidase, from the cell pellet and conditioned medium of Bacillus circulans, by sequential chromatography using DE52 and phenyl-Sepharose columns with approximately 63- and 180-fold purity and 58 and 12.5% recovery, respectively.
T4 609-643 Sentence denotes Like keratanase II of Bacillus sp.
T5 644-897 Sentence denotes Ks36, the enzyme, designated Bc keratanase II, hydrolyzed KS between the 4GlcNAcbeta1-3Gal1 structure (endo-beta-N-acetylglucosaminidase), but not hyaluronan, heparan sulfate, heparin, and chondroitin sulfate C, demonstrating a strict specificity to KS.
T6 898-1061 Sentence denotes The enzyme digested shark cartilage KS to disaccharides and tetrasaccharides and bovine cornea KS to hexasaccharide, indicating that it prefers highly sulfated KS.
T7 1062-1352 Sentence denotes Distinct from keratanase II of strain Ks36, the enzyme digested shark cartilage KS at an optimal temperature of 55 degrees C. Based on partial peptide sequencing of the enzyme, we molecularly cloned the gene, which encodes a protein with a predicted molecular mass of approximately 200 kDa.
T8 1353-1690 Sentence denotes From the deduced protein sequence, Bc keratanase II contained a domain at the C terminus, homologous to the S-layer-like domain of pullulanase from Thermoanaerobacterium thermosulfurigenes and endoxylanase from Thermoanaerobacterium saccharolyticum, and a carbohydrate-binding domain, which may serve to specifically recognize KS chains.
T9 1691-1754 Sentence denotes A full-length recombinant enzyme showed keratanase II activity.
T10 1755-1870 Sentence denotes These results may prove useful for the structural analysis of KS toward achieving an understanding of its function.

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 65-80 Glycan denotes keratan sulfate https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T2 152-167 Glycan denotes Keratan sulfate https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T3 169-171 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T4 316-318 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T5 340-342 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T6 702-704 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T7 833-844 Glycan denotes chondroitin https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT
T8 894-896 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T9 934-936 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T10 993-995 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T11 1058-1060 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T12 1142-1144 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T13 1680-1682 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T14 1817-1819 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T22 803-818 Glycan denotes heparan sulfate https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 65-80 Glycan denotes keratan sulfate https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T2 152-167 Glycan denotes Keratan sulfate https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T3 169-171 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T4 316-318 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T5 340-342 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T6 702-704 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T7 833-844 Glycan denotes chondroitin https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT
T8 894-896 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T9 934-936 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T10 993-995 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T11 1058-1060 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T12 1142-1144 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T13 1680-1682 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T14 1817-1819 Glycan denotes KS https://api.glycosmos.org/wurcs2image/latest/png/binary/G82109MW
T22 803-818 Glycan denotes heparan sulfate https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW

GlyCosmos15-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 65-80 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes keratan sulfate http://www.glycoepitope.jp/epitopes/EP0085
T2 152-167 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes Keratan sulfate http://www.glycoepitope.jp/epitopes/EP0085
T3 169-171 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T4 316-318 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T5 340-342 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T6 702-704 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T7 803-818 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes heparan sulfate http://www.glycoepitope.jp/epitopes/EP0086
T8 833-844 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes chondroitin http://www.glycoepitope.jp/epitopes/EP0081
T9 894-896 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T10 934-936 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T11 993-995 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T12 1058-1060 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T13 1142-1144 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T14 1680-1682 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085
T15 1817-1819 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes KS http://www.glycoepitope.jp/epitopes/EP0085

GlyCosmos15-Lectin-Jamboree

Id Subject Object Predicate Lexical cue glycosmos_id
T1 1484-1495 Lectin denotes pullulanase GL_001796

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 132-140 OrganismTaxon denotes Bacillus 1386|55087
T3 436-444 OrganismTaxon denotes Bacillus 1386|55087
T5 631-639 OrganismTaxon denotes Bacillus 1386|55087
T7 979-985 OrganismTaxon denotes bovine 9913
T8 1501-1541 OrganismTaxon denotes Thermoanaerobacterium thermosulfurigenes 33950
T9 1564-1601 OrganismTaxon denotes Thermoanaerobacterium saccharolyticum 28896

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 403-409 Body_part denotes pellet http://purl.obolibrary.org/obo/UBERON_0036018
T2 924-933 Body_part denotes cartilage http://purl.obolibrary.org/obo/UBERON_0002418|http://purl.obolibrary.org/obo/UBERON_0007844
T4 986-992 Body_part denotes cornea http://purl.obolibrary.org/obo/UBERON_0000964
T5 1132-1141 Body_part denotes cartilage http://purl.obolibrary.org/obo/UBERON_0002418|http://purl.obolibrary.org/obo/UBERON_0007844
T7 1463-1468 Body_part denotes layer http://purl.obolibrary.org/obo/UBERON_0000119|http://purl.obolibrary.org/obo/UBERON_0022303