PubMed:12728302 JSONTXT

{"project":"bionlp-st-bb3-2016-training","denotations":[{"id":"T1","span":{"begin":0,"end":123},"obj":"Title"},{"id":"T2","span":{"begin":124,"end":1248},"obj":"Paragraph"},{"id":"T3","span":{"begin":98,"end":122},"obj":"Bacteria"},{"id":"T4","span":{"begin":136,"end":152},"obj":"Bacteria"},{"id":"T5","span":{"begin":174,"end":178},"obj":"Habitat"},{"id":"T6","span":{"begin":194,"end":218},"obj":"Bacteria"},{"id":"T7","span":{"begin":457,"end":481},"obj":"Bacteria"},{"id":"T8","span":{"begin":661,"end":668},"obj":"Bacteria"},{"id":"T9","span":{"begin":791,"end":799},"obj":"Bacteria"},{"id":"T10","span":{"begin":820,"end":824},"obj":"Bacteria"},{"id":"T11","span":{"begin":961,"end":968},"obj":"Bacteria"}],"text":"Heat-shock response and its contribution to thermotolerance of the nitrogen-fixing cyanobacterium Anabaena sp. strain L-31.\nCompared to Escherichia coli, the nitrogen-fixing soil cyanobacterium Anabaena sp. strain L-31 exhibited significantly superior abilities to survive prolonged and continuous heat stress and recover therefrom. Temperature upshift induced the synthesis of heat-shock proteins of similar molecular mass in the two microbes. However, in Anabaena sp. strain L-31 the heat-shock proteins (particularly the GroEL proteins) were synthesised throughout the stress period, were much more stable and accumulated during heat stress. In contrast, in E. coli the heat-shock proteins were transiently synthesised, quickly turned over and did not accumulate. Nitrogenase activity of Anabaena cells of sp. strain L-31 continuously exposed to heat stress for 7 days rapidly recovered from thermal injury, although growth recovery was delayed. Exposure of E. coli cells to \u003e4.5 h of heat stress resulted in a complete loss of viability and the ability to recover. Marked differences in the synthesis, stability and accumulation of heat-shock proteins appear to distinguish these bacteria in their thermotolerance and recovery from heat stress."}