PubMed:12725691 JSONTXT

{"project":"PennBioIE","denotations":[{"id":"T1","span":{"begin":0,"end":3},"obj":"protein"},{"id":"T2","span":{"begin":24,"end":40},"obj":"protein"},{"id":"T3","span":{"begin":55,"end":58},"obj":"protein"},{"id":"T4","span":{"begin":119,"end":122},"obj":"protein"},{"id":"T5","span":{"begin":130,"end":139},"obj":"protein"},{"id":"T6","span":{"begin":163,"end":166},"obj":"protein"},{"id":"T7","span":{"begin":204,"end":213},"obj":"protein"},{"id":"T8","span":{"begin":241,"end":254},"obj":"protein"},{"id":"T9","span":{"begin":299,"end":302},"obj":"protein"},{"id":"T10","span":{"begin":329,"end":332},"obj":"protein"},{"id":"T11","span":{"begin":396,"end":405},"obj":"protein"},{"id":"T12","span":{"begin":446,"end":449},"obj":"protein"},{"id":"T13","span":{"begin":477,"end":486},"obj":"protein"},{"id":"T14","span":{"begin":514,"end":523},"obj":"protein"},{"id":"T15","span":{"begin":527,"end":530},"obj":"protein"},{"id":"T16","span":{"begin":556,"end":559},"obj":"protein"},{"id":"T17","span":{"begin":618,"end":633},"obj":"protein"},{"id":"T18","span":{"begin":671,"end":680},"obj":"protein"},{"id":"T19","span":{"begin":711,"end":714},"obj":"protein"},{"id":"T20","span":{"begin":773,"end":776},"obj":"protein"},{"id":"T21","span":{"begin":801,"end":810},"obj":"protein"},{"id":"T22","span":{"begin":834,"end":837},"obj":"protein"},{"id":"T23","span":{"begin":860,"end":873},"obj":"protein"}],"text":"PKR is not required for interferon-gamma inhibition of VSV replication in neurons.\nIn this report, the contribution of PKR to the IFN-gamma mediated inhibition of VSV replication in neurons was examined. IFN-gamma treatment of NB41A3 murine neuroblastoma cells resulted in the reduced expression of VSV protein during infection. PKR was found to be modestly upregulated in NB41A3 cells following IFN-gamma treatment. The phosphorylation state of PKR and its downstream target, eIF2alpha, were unaffected by either IFN-gamma or VSV infection. Inhibition of PKR through the use of 2-aminopurine or the expression of the Influenza A NS1 gene had no effect on the ability of IFN-gamma to inhibit the replication of VSV in vitro. These data indicate that endogenously expressed PKR is not required for the IFN-gamma mediated inhibition of VSV replication in NB41A3 neuroblastoma cells."}