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PubMed:12682047 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-107 Sentence denotes YMR313c/TGL3 encodes a novel triacylglycerol lipase located in lipid particles of Saccharomyces cerevisiae.
T2 108-202 Sentence denotes Previous work from our laboratory (Athenstaedt, K., Zweytick, D., Jandrositz, A., Kohlwein, S.
T3 203-229 Sentence denotes D., and Daum, G. (1999) J.
T4 230-240 Sentence denotes Bacteriol.
T5 241-445 Sentence denotes 181, 6441-6448) showed that the gene product of YMR313c (named Tgl3p) is a component of yeast lipid particles, and deletion of this gene led to an increase in the cellular level of triacylglycerols (TAG).
T6 446-537 Sentence denotes These observations suggested that TGL3 may encode a TAG lipase of Saccharomyces cerevisiae.
T7 538-764 Sentence denotes Here we demonstrate by cell fractionation and by microscopic inspection of a strain bearing a Tgl3p-GFP hybrid that this polypeptide is highly enriched in the lipid particle fraction but virtually absent from other organelles.
T8 765-1001 Sentence denotes The entire TAG lipase activity of lipid particles is attributed to Tgl3p, because the activity in this organelle is completely absent in a Deltatgl3 deletion mutant, whereas it is significantly enhanced in a strain overexpressing Tgl3p.
T9 1002-1149 Sentence denotes A His6-tagged Tgl3p hybrid purified close to homogeneity from a yeast strain overexpressing this fusion protein exhibited high TAG lipase activity.
T10 1150-1339 Sentence denotes Most importantly, experiments in vivo using the fatty acid synthesis inhibitor cerulenin demonstrated that deletion of TGL3 resulted in a decreased mobilization of TAG from lipid particles.
T11 1340-1482 Sentence denotes The amino acid sequence deduced from the open reading frame YMR313c contains the consensus sequence motif GXSXG typical for lipolytic enzymes.
T12 1483-1572 Sentence denotes Otherwise, Tgl3p has no significant sequence homology to other lipases identified so far.
T13 1573-1699 Sentence denotes In summary, our data identified Tgl3p as a novel yeast TAG lipase at the molecular level and by function in vivo and in vitro.

LocText

Id Subject Object Predicate Lexical cue
T1 0-7 uniprot:P40308 denotes YMR313c
T2 8-12 uniprot:P40308 denotes TGL3
T3 63-78 go:GO:0005811 denotes lipid particles
T4 82-106 taxonomy:4932 denotes Saccharomyces cerevisiae
T5 289-296 uniprot:P40308 denotes YMR313c
T6 304-309 uniprot:P40308 denotes Tgl3p
T7 329-334 taxonomy:4932 denotes yeast
T8 335-350 go:GO:0005811 denotes lipid particles
T9 480-484 uniprot:P40308 denotes TGL3
T10 512-536 taxonomy:4932 denotes Saccharomyces cerevisiae
T11 632-637 uniprot:P40308 denotes Tgl3p
T12 638-641 uniprot:uniprot: denotes GFP
T13 697-711 go:GO:0005811 denotes lipid particle
T14 799-814 go:GO:0005811 denotes lipid particles
T15 832-837 uniprot:P40308 denotes Tgl3p
T16 904-913 uniprot:uniprot: denotes Deltatgl3
T17 995-1000 uniprot:P40308 denotes Tgl3p
T18 1004-1008 uniprot:uniprot: denotes His6
T19 1016-1021 uniprot:P40308 denotes Tgl3p
T20 1066-1071 taxonomy:4932 denotes yeast
T21 1269-1273 uniprot:P40308 denotes TGL3
T22 1323-1338 go:GO:0005811 denotes lipid particles
T23 1400-1407 uniprot:P40308 denotes YMR313c
T24 1494-1499 uniprot:P40308 denotes Tgl3p
T25 1605-1610 uniprot:P40308 denotes Tgl3p
T26 1622-1627 taxonomy:4932 denotes yeast
R1 T1 T3 localizeTo YMR313c,lipid particles
R2 T2 T3 localizeTo TGL3,lipid particles
R3 T5 T8 localizeTo YMR313c,lipid particles
R4 T6 T8 localizeTo Tgl3p,lipid particles
R5 T9 T13 localizeTo TGL3,lipid particle
R6 T11 T13 localizeTo Tgl3p,lipid particle
R7 T15 T14 localizeTo Tgl3p,lipid particles
R8 T16 T14 localizeTo Deltatgl3,lipid particles
R9 T23 T22 localizeTo YMR313c,lipid particles

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 82-106 OrganismTaxon denotes Saccharomyces cerevisiae 4932
T2 512-536 OrganismTaxon denotes Saccharomyces cerevisiae 4932

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 753-763 Body_part denotes organelles http://purl.obolibrary.org/obo/GO_0043226
T2 868-877 Body_part denotes organelle http://purl.obolibrary.org/obo/GO_0043226